| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 834,048 | G→C | 20.8% | E836Q (GAA→CAA) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 834,048 | 0 | G | C | 20.8% | 144.3 / 32.8 | 72 | E836Q (GAA→CAA) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base G (32/25); new base C (5/10); total (37/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
GTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCT > Exported/833982‑834113 | gTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAgt < 1:593408/71‑1 (MQ=255) gATCCGGCGTGGGAATCACGCAGCGACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGtgtg < 1:4110679/71‑1 (MQ=255) aTCCGGCGTGGGAATCACGCAGCGACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGc < 1:1316784/71‑1 (MQ=255) aTCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAgtgt < 1:2696619/69‑1 (MQ=255) aTCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGc > 1:217339/1‑71 (MQ=255) tCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCg > 1:1222129/1‑71 (MQ=255) tCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCg > 1:1893072/1‑71 (MQ=255) ccGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGt > 1:3690035/1‑71 (MQ=255) cGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGt < 1:5064572/70‑1 (MQ=255) cGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTggg > 1:4726129/1‑70 (MQ=255) gAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGa < 1:5235413/48‑1 (MQ=255) gAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGt > 1:2200177/1‑70 (MQ=255) gcaGCGACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGCGTCATCTTGGCaaa < 1:540376/69‑1 (MQ=255) aaaGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTa > 1:1998257/1‑69 (MQ=255) aaGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTa > 1:3336904/1‑68 (MQ=255) aaGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAg < 1:2508654/71‑1 (MQ=255) aaGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAg < 1:3145946/71‑1 (MQ=255) aGCGACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGTCAAAGa < 1:729744/71‑1 (MQ=255) gCGACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGtgtg < 1:5248954/49‑1 (MQ=255) gCGACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCaaa < 1:1676309/68‑1 (MQ=255) gCGACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCaaa < 1:2248638/68‑1 (MQ=255) gACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCAAAGa > 1:3940128/1‑68 (MQ=255) gACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCAAAGa > 1:3160282/1‑68 (MQ=255) aCTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAAcc < 1:3537040/71‑1 (MQ=255) cTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCg > 1:4321152/1‑47 (MQ=255) cTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGCTCATCTTGGCAAAGAAAc > 1:2743144/1‑69 (MQ=255) gggAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCAAAGAAACCGAc > 1:4552370/1‑71 (MQ=255) ggAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCAAAGAAACCGAc < 1:2783832/70‑1 (MQ=255) ggAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCAAAGAAACCGAc < 1:2258563/70‑1 (MQ=255) ggAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAgtgt > 1:5436248/1‑41 (MQ=255) ggAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGAc < 1:2862128/70‑1 (MQ=255) gAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCAAAGAAACCGAcgt < 1:2618031/71‑3 (MQ=255) aTCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGAc < 1:4122724/66‑1 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGt < 1:2503888/70‑1 (MQ=255) cTACAAAGCCATCGCGAAGAAATTCTCCGAAgtgt > 1:2171580/1‑35 (MQ=255) cTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGa > 1:4100183/1‑57 (MQ=255) cTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACAt > 1:1844271/1‑66 (MQ=255) cTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCa > 1:5401601/1‑71 (MQ=255) tACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGa > 1:1545818/1‑62 (MQ=255) tACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCAc > 1:2226527/1‑71 (MQ=255) aCAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGt > 1:2651771/1‑37 (MQ=255) aCAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCa > 1:2529147/1‑69 (MQ=255) cAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCAc < 1:1865711/69‑1 (MQ=255) cAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACg > 1:1021126/1‑70 (MQ=255) cAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGc < 1:2239059/71‑1 (MQ=255) aaaGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCg < 1:252611/58‑1 (MQ=255) aaaGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCg < 1:1063994/58‑1 (MQ=255) aaaGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCg < 1:4628610/58‑1 (MQ=255) aaGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTaaa > 1:645874/1‑50 (MQ=255) aaGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTaaa > 1:1495186/1‑50 (MQ=255) tCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATc < 1:4375163/56‑1 (MQ=255) cgcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATc > 1:4370572/1‑70 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACAt > 1:1707007/1‑53 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACAt > 1:3755340/1‑53 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATc > 1:2810237/1‑69 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATc > 1:226926/1‑69 (MQ=255) cgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTaaa > 1:5557138/1‑41 (MQ=255) cgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAg > 1:1941701/1‑71 (MQ=255) agaaATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCa > 1:4731918/1‑54 (MQ=255) agaaATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCAt < 1:2440358/71‑1 (MQ=255) gaaATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCAt < 1:1024992/70‑1 (MQ=255) aaaTTCTCCGAAGTGTGCGTCGGCCATCTGGGTaaa < 1:1826759/36‑1 (MQ=255) aaaTTCTCCGAAGTGTGCGTCGGCCATCTGGGTaaa < 1:3509166/36‑1 (MQ=255) aaaTTCTCCGAAGTGTGCGTCGGCCATCTGGGTaaa < 1:4792115/36‑1 (MQ=255) aaaTTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGa < 1:4965660/71‑1 (MQ=255) aTTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAAcc > 1:2216898/1‑40 (MQ=255) tctcCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTAt < 1:3799150/58‑1 (MQ=255) tctcCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTAt < 1:2527302/58‑1 (MQ=255) tctcCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTAt < 1:1505163/58‑1 (MQ=255) tctcCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGActct > 1:2784750/1‑71 (MQ=255) tctcCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGActct > 1:101428/1‑71 (MQ=255) tctcCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGActc > 1:2851460/1‑70 (MQ=255) | GTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCT > Exported/833982‑834113 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |