| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 834,071 | T→C | 13.4% | G843G (GGT→GGC) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 834,071 | 0 | T | C | 13.4% | 199.5 / 19.5 | 82 | G843G (GGT→GGC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (35/36); new base C (4/7); total (39/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01 | |||||||||||
GAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGAT > Exported/834002‑834140 | gAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGt > 1:2200177/1‑70 (MQ=255) gcaGCGACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGCGTCATCTTGGCaaa < 1:540376/69‑1 (MQ=255) aaaGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTa > 1:1998257/1‑69 (MQ=255) aaGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTa > 1:3336904/1‑68 (MQ=255) aaGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAg < 1:3145946/71‑1 (MQ=255) aaGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAg < 1:2508654/71‑1 (MQ=255) aGCGACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGTCAAAGa < 1:729744/71‑1 (MQ=255) gCGACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCaaa < 1:2248638/68‑1 (MQ=255) gCGACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCaaa < 1:1676309/68‑1 (MQ=255) gACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCAAAGa > 1:3940128/1‑68 (MQ=255) gACTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCAAAGa > 1:3160282/1‑68 (MQ=255) aCTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAAcc < 1:3537040/71‑1 (MQ=255) cTGGGAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGCTCATCTTGGCAAAGAAAc > 1:2743144/1‑69 (MQ=255) gggAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCAAAGAAACCGAc > 1:4552370/1‑71 (MQ=255) ggAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCAAAGAAACCGAc < 1:2783832/70‑1 (MQ=255) ggAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCAAAGAAACCGAc < 1:2258563/70‑1 (MQ=255) ggAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGAc < 1:2862128/70‑1 (MQ=255) gAAATCTACAAAGGTATCGCCAAAGCATTTTCGCAAGTGTGCGTGGGTCATCTTGGCAAAGAAACCGAcgt < 1:2618031/71‑3 (MQ=255) aTCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGAc < 1:4122724/66‑1 (MQ=255) tCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGt < 1:2503888/70‑1 (MQ=255) cTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGa > 1:4100183/1‑57 (MQ=255) cTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACAt > 1:1844271/1‑66 (MQ=255) cTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCa > 1:5401601/1‑71 (MQ=255) tACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGa > 1:1545818/1‑62 (MQ=255) tACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCAc > 1:2226527/1‑71 (MQ=255) aCAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCa > 1:2529147/1‑69 (MQ=255) cAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCAc < 1:1865711/69‑1 (MQ=255) cAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACg > 1:1021126/1‑70 (MQ=255) cAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGc < 1:2239059/71‑1 (MQ=255) aaaGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCg < 1:1063994/58‑1 (MQ=255) aaaGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCg < 1:4628610/58‑1 (MQ=255) aaaGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCg < 1:252611/58‑1 (MQ=255) aaGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTaaa > 1:1495186/1‑50 (MQ=255) aaGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTaaa > 1:645874/1‑50 (MQ=255) tCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATc < 1:4375163/56‑1 (MQ=255) cgcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATc > 1:4370572/1‑70 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACAt > 1:3755340/1‑53 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACAt > 1:1707007/1‑53 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATc > 1:226926/1‑69 (MQ=255) gcgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATc > 1:2810237/1‑69 (MQ=255) cgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTaaa > 1:5557138/1‑41 (MQ=255) cgAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAg > 1:1941701/1‑71 (MQ=255) agaaATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCa > 1:4731918/1‑54 (MQ=255) agaaATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCAt < 1:2440358/71‑1 (MQ=255) gaaATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCAt < 1:1024992/70‑1 (MQ=255) aaaTTCTCCGAAGTGTGCGTCGGCCATCTGGGTaaa < 1:3509166/36‑1 (MQ=255) aaaTTCTCCGAAGTGTGCGTCGGCCATCTGGGTaaa < 1:1826759/36‑1 (MQ=255) aaaTTCTCCGAAGTGTGCGTCGGCCATCTGGGTaaa < 1:4792115/36‑1 (MQ=255) aaaTTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGa < 1:4965660/71‑1 (MQ=255) aTTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAAcc > 1:2216898/1‑40 (MQ=255) tctcCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTAt < 1:2527302/58‑1 (MQ=255) tctcCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTAt < 1:3799150/58‑1 (MQ=255) tctcCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTAt < 1:1505163/58‑1 (MQ=255) tctcCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGActct > 1:2784750/1‑71 (MQ=255) tctcCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGActct > 1:101428/1‑71 (MQ=255) tctcCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGActc > 1:2851460/1‑70 (MQ=255) aGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCt > 1:5064887/1‑70 (MQ=255) aGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCt > 1:1922530/1‑70 (MQ=255) aGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTg < 1:1136116/71‑1 (MQ=255) aGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTg < 1:328831/71‑1 (MQ=255) aGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTg < 1:4679563/71‑1 (MQ=255) gtgtGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTg > 1:1337189/1‑70 (MQ=255) gtgtGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTg > 1:520391/1‑70 (MQ=255) gtgtGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTg > 1:5307175/1‑70 (MQ=255) gtgCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGc > 1:183619/1‑41 (MQ=255) tgCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAg < 1:4672705/51‑1 (MQ=255) tgCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACt < 1:4136320/71‑1 (MQ=255) tgCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACt < 1:1331336/71‑1 (MQ=255) gCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTg > 1:5150514/1‑71 (MQ=255) cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCAt < 1:1568460/52‑1 (MQ=255) cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCAt < 1:2588099/52‑1 (MQ=255) cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCAt < 1:1278332/52‑1 (MQ=255) cGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTgg > 1:2060598/1‑71 (MQ=255) ggCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGgcg > 1:1227394/1‑69 (MQ=255) tCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATc < 1:1834621/37‑1 (MQ=255) tCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTg < 1:1809211/61‑1 (MQ=255) cTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCt > 1:4699909/1‑71 (MQ=255) tGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTg > 1:551687/1‑71 (MQ=255) ggTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTg < 1:4209452/69‑1 (MQ=255) ggTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGa < 1:4554914/71‑1 (MQ=255) gTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCAt < 1:3350633/38‑1 (MQ=255) gTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCAt < 1:2348597/38‑1 (MQ=255) gTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGAt > 1:563997/1‑71 (MQ=255) tAAAGAAACCGACATCGTCACGCTGCCTATCCAGCAt < 1:2279613/37‑1 (MQ=255) tAAAGAAACCGACATCGTCACGCTGCCTATCCAGCAt < 1:2838668/37‑1 (MQ=255) | GAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGAT > Exported/834002‑834140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |