| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 834,249 | A→C | 26.7% | I903L (ATC→CTC) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 834,249 | 0 | A | C | 26.7% | 68.4 / 44.3 | 60 | I903L (ATC→CTC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (16/28); new base C (5/11); total (21/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01 | |||||||||||
AGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGA > Exported/834179‑834319 | aGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTa > 1:4131261/1‑71 (MQ=255) aaaaCCTCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAACGCTTTACCTCGCTCgg > 1:3373877/1‑71 (MQ=255) aaaaCCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAACGCTTTACCTCGCTCgg < 1:537943/71‑1 (MQ=255) aaaaCCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAACGCTTTACCTCGCTCgg > 1:2028724/1‑71 (MQ=255) aaaaCCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAACGCTTTACCTCGCTCgg > 1:4059875/1‑71 (MQ=255) aaaaCCGCGCCGAATATTGTGGCGGTGGAGCGCGACTACCCTGCTACGTATGAACGCTTTACCTCGCTCgg > 1:3264816/1‑71 (MQ=255) gcgcCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCt < 1:151129/71‑1 (MQ=255) acaTTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATg > 1:2989700/1‑68 (MQ=255) caTTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTa > 1:3775983/1‑53 (MQ=255) ttATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGaaa < 1:276779/71‑1 (MQ=255) tGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTAt > 1:1417611/1‑49 (MQ=255) gTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCgg < 1:512139/71‑1 (MQ=255) gTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCgg < 1:2863443/71‑1 (MQ=255) tCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGGGAAAATCGGt < 1:918393/71‑1 (MQ=255) tCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGt < 1:2213556/71‑1 (MQ=255) tCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGt < 1:4618116/71‑1 (MQ=255) gTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTaa > 1:2423121/1‑71 (MQ=255) agagCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATg > 1:2292926/1‑71 (MQ=255) agagCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATg > 1:1718139/1‑71 (MQ=255) gagCGCGACTACCCTGCTACGTATGAACGCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATgg < 1:2331647/71‑1 (MQ=255) gagCGCGACTACCCTGCTACGTATGAACGCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATgg < 1:2770130/71‑1 (MQ=255) agCGCGACTACCCTGCTACGTATGAACGCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATGGc < 1:2732854/71‑1 (MQ=255) cgcgATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAAt < 1:2180099/59‑1 (MQ=255) cgATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGt > 1:2191912/1‑70 (MQ=255) aTTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCgg < 1:3378964/58‑1 (MQ=255) ttATCCGGCGACTTACGAACGCTTTACCTCTATCGGc > 1:3829941/1‑37 (MQ=255) ttATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTaa > 1:5053776/1‑60 (MQ=255) ttATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGt > 1:2175296/1‑67 (MQ=255) ttATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAg > 1:4055910/1‑71 (MQ=255) aTCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCt < 1:4876335/40‑1 (MQ=255) aTCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAgg < 1:3738083/70‑1 (MQ=255) cGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAgg < 1:4685043/67‑1 (MQ=255) ggCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTg < 1:2770132/71‑1 (MQ=255) aCTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGG‑AAAGGGATTGCCTgg < 1:419840/71‑1 (MQ=255) ttACAAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAggg < 1:3928344/61‑1 (MQ=255) aCGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGaa < 1:291860/70‑1 (MQ=255) tgAACGCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAAt < 1:271263/45‑1 (MQ=255) aaCGCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATGGCGGTAAAGGGATTTCGTgg < 1:267508/65‑1 (MQ=255) aaCGCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATGGCGGTAAAGGGATTTCGTGGAATAcc > 1:4518783/1‑71 (MQ=255) cGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGt < 1:1646611/39‑1 (MQ=255) cGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGt < 1:3049915/39‑1 (MQ=255) cGCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATGGCGGTAAAggg < 1:584154/54‑1 (MQ=255) gCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATGGCGGTAAAGGGa < 1:2556674/54‑1 (MQ=255) gCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATGGCGGTAAAGGGa < 1:2753986/54‑1 (MQ=255) gCTTTACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATGGCGGTAAAGGGATTTCGTgg < 1:3178416/62‑1 (MQ=255) cTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTaa > 1:2996780/1‑39 (MQ=255) cTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTaa > 1:3443090/1‑39 (MQ=255) cTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTaa > 1:4420579/1‑39 (MQ=255) cTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGCTAATGGCGGTAAAGGGATTGCCTGGAACACCCaga < 1:761213/71‑1 (MQ=255) tttACCTCTATCGGCCCGCGGATGGAGAAAATCGGTa > 1:2821064/1‑37 (MQ=255) ttACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGc < 1:1780629/55‑1 (MQ=255) ttACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGc < 1:2732616/55‑1 (MQ=255) tACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTa > 1:2957626/1‑44 (MQ=255) tACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGc < 1:3115838/70‑1 (MQ=255) tACCTCGCTCGGGCCATTGATGGACAAACTTGGCAATGGCGGTAAAGGGATTTCGTGGAATACCCaggat < 1:4847850/70‑4 (MQ=255) aCCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCg < 1:5329022/70‑1 (MQ=255) ccTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCga < 1:2660199/70‑1 (MQ=255) ccTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCga < 1:3160474/70‑1 (MQ=255) ctctATCGGCCCGCTGATGGAGAAAATCGGTAATgg < 1:2364127/36‑1 (MQ=255) ctctATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCgaga < 1:4396547/71‑1 (MQ=255) ctATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGc < 1:210801/65‑1 (MQ=255) tATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATg > 1:1766222/1‑70 (MQ=255) aTCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGa > 1:894272/1‑71 (MQ=255) aTCGGCCCGCTGATGGAGAAAATAGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGa > 1:4372801/1‑71 (MQ=255) | AGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGA > Exported/834179‑834319 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |