| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 834,389 | A→C | 9.5% | A949A (GCA→GCC) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 834,389 | 0 | A | C | 9.5% | 174.3 / 13.0 | 74 | A949A (GCA→GCC) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (38/29); new base C (3/4); total (41/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01 | |||||||||||
TCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCC > Exported/834320‑834455 | tCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCaa > 1:845866/1‑71 (MQ=255) tgctgcGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAAtt > 1:181183/1‑71 (MQ=255) ctgcGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCa < 1:3101099/66‑1 (MQ=255) ctgcGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCa < 1:5326796/66‑1 (MQ=255) ctgcGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCa < 1:5333362/66‑1 (MQ=255) ctgcGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAAtt < 1:884910/69‑1 (MQ=255) tgcGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGAt > 1:1985634/1‑71 (MQ=255) cGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATg < 1:5159508/70‑1 (MQ=255) gTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGc > 1:850802/1‑70 (MQ=255) tAAGCTCAACTACACCAAAGCGGAAGGTCCGGCAAAAGGCCAGCCGATGCTCAATACCGCAATTGATGCgg < 1:5399106/71‑1 (MQ=255) tAAGCTCAACTACACCAAAGCGGAAGGTCCAGCAAAAGGCCAGCCGATGCTCAATACCGCAATTGATGCgg < 1:2879084/71‑1 (MQ=255) aaGCTCAACTACACCAAAGCGGAAGGTCCGGCAAAAGGCCAGCCGATGCTCAATACCGCAATTGATGCgg < 1:3022561/70‑1 (MQ=255) gCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCag < 1:3327662/71‑1 (MQ=255) tCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCa > 1:3608361/1‑68 (MQ=255) tCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCa > 1:365340/1‑68 (MQ=255) aaCTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAgaga < 1:4914177/70‑1 (MQ=255) aCTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAAtt < 1:523936/56‑1 (MQ=255) aCTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAgatg < 1:961108/71‑1 (MQ=255) aCTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAgatg < 1:2228065/71‑1 (MQ=255) acacCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATc > 1:5476158/1‑71 (MQ=255) cacCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATcc < 1:5451545/71‑1 (MQ=255) acCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCt > 1:3485436/1‑71 (MQ=255) ccAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCaa > 1:75642/1‑48 (MQ=255) cAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCg < 1:4539773/55‑1 (MQ=255) cAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATc > 1:4108804/1‑67 (MQ=255) cAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTg > 1:983200/1‑70 (MQ=255) cAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGa > 1:2804152/1‑71 (MQ=255) aGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTg > 1:1750231/1‑47 (MQ=255) aGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTg < 1:3280953/67‑1 (MQ=255) aGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTg < 1:3049245/67‑1 (MQ=255) aGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGacac < 1:799572/71‑1 (MQ=255) aGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGacac > 1:3474654/1‑71 (MQ=255) aGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGacac > 1:3238812/1‑71 (MQ=255) aGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGacac > 1:117271/1‑71 (MQ=255) aGCGGAAGGTCCGGCGAAAGGACAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGacac > 1:2200624/1‑71 (MQ=255) cGGAAGGTCCGGGGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGac > 1:4328888/1‑67 (MQ=255) cGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATTATCCTGACACTg < 1:5524038/71‑1 (MQ=255) cGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGac > 1:4090681/1‑67 (MQ=255) gAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGc < 1:3128083/71‑1 (MQ=255) aaGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGaca > 1:4045272/1‑65 (MQ=255) aaGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGac > 1:5615062/1‑64 (MQ=255) ggTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCAcc > 1:2905575/1‑71 (MQ=255) ggTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCAcc > 1:2593234/1‑71 (MQ=255) ggTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCAcc > 1:5262326/1‑71 (MQ=255) ggTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCAcc > 1:279286/1‑71 (MQ=255) ggTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCAcc > 1:1892218/1‑71 (MQ=255) cGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGa > 1:2987085/1‑70 (MQ=255) ggCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCaga < 1:2506126/45‑1 (MQ=255) ggCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCaga < 1:193058/45‑1 (MQ=255) aaGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAgatgat > 1:2462898/1‑46 (MQ=255) aaGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAgatgat > 1:5111066/1‑46 (MQ=255) aaGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAgatgat > 1:2982068/1‑46 (MQ=255) aGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACg > 1:1376679/1‑71 (MQ=255) ccAGCCGATGCTGAACACCGCAATTGATGC‑‑CAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAg < 1:482382/71‑1 (MQ=255) aGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAg < 1:2406312/71‑1 (MQ=255) ccGATGATTGACACCGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGaaa > 1:246601/1‑58 (MQ=255) tGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTTAGGTAGcc > 1:3078595/1‑71 (MQ=255) gCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGa < 1:4031152/51‑1 (MQ=255) gCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGa < 1:1163626/51‑1 (MQ=255) gCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGa < 1:2659106/51‑1 (MQ=255) gCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGa < 1:2746630/51‑1 (MQ=255) gCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGa < 1:3195754/51‑1 (MQ=255) gCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACg > 1:5672233/1‑59 (MQ=255) gCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCg > 1:524524/1‑71 (MQ=255) cTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCAGAAACCAACGGTCAGGTAGCCGt > 1:2728700/1‑71 (MQ=255) ttgacacCGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGt > 1:3927219/4‑70 (MQ=255) gacacCGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGtca > 1:2513408/2‑68 (MQ=255) aaCACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAg > 1:2180490/1‑64 (MQ=255) aaCACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTg > 1:1513117/1‑69 (MQ=255) aaCACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGa > 1:1088208/1‑70 (MQ=255) aaCACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGa > 1:1921058/1‑70 (MQ=255) acacCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCAc > 1:3256172/1‑42 (MQ=255) acCGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGc < 1:5314949/71‑1 (MQ=255) acCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCACCGGTCAGGTAGCCGTGAAAGc < 1:5325265/71‑1 (MQ=255) acCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGc < 1:2065873/71‑1 (MQ=255) ccGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGcg < 1:2907865/71‑2 (MQ=255) ccGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGcg < 1:4829589/71‑2 (MQ=255) ccGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGcc < 1:2940260/71‑1 (MQ=255) cGCCATTGACGCTTCAGAAGTGATTCTGGCACTGGCACCGGAAACCAACGGTCATGTTGCAGTCAAAGcg < 1:2075062/70‑2 (MQ=255) | TCTGCTGCGTAAGCTCAACTACACCAAAGCGGAAGGTCCGGCGAAAGGCCAGCCGATGCTGAACACCGCAATTGATGCGGCAGAGATGATCCTGACACTGGCACCGGAAACCAACGGTCAGGTAGCCGTGAAAGCC > Exported/834320‑834455 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |