| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 190,065 | G→A | 26.0% | R521H (CGT→CAT) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 190,065 | 0 | G | A | 26.0% | 82.0 / 27.1 | 50 | R521H (CGT→CAT) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (21/16); new base A (4/9); total (25/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01 | |||||||||||
AATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATA > Exported/190010‑190130 | aaTATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTaga < 1:4745519/71‑1 (MQ=255) tGAGCGAGGAGGGTATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGaa < 1:2350713/70‑1 (MQ=255) tGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTagag > 1:5387258/1‑68 (MQ=255) tGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGaa > 1:1456284/1‑70 (MQ=255) tGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGaa < 1:2023902/70‑1 (MQ=255) aGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCgtag > 1:2358403/1‑61 (MQ=255) aGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCgta > 1:442191/1‑60 (MQ=255) aggaggGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGc > 1:3891542/1‑71 (MQ=255) cccGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCgt < 1:5321746/45‑1 (MQ=255) cccGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCgt < 1:2847685/45‑1 (MQ=255) cggcggCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCgtag > 1:3050286/1‑45 (MQ=255) cggcggCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCgtag > 1:641688/1‑45 (MQ=255) cggcggCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACctg > 1:4643796/1‑71 (MQ=255) ggcggcGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTta < 1:1127544/63‑1 (MQ=255) ggcggcGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACctgc < 1:1885781/71‑1 (MQ=255) tGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTtctc > 1:5501753/1‑71 (MQ=255) gTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCg > 1:3409359/1‑46 (MQ=255) gTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGcc < 1:3979568/49‑1 (MQ=255) gTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGcc < 1:3856959/49‑1 (MQ=255) tAGCAAAATTCCTCGACGAACGTGGGATCGTAGTaga < 1:5169902/37‑1 (MQ=255) cAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCtt < 1:4343062/48‑1 (MQ=255) cAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCtt < 1:3965627/48‑1 (MQ=255) cAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTa < 1:4080404/69‑1 (MQ=255) cAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAg > 1:3135692/1‑70 (MQ=255) gaaaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:1921034/70‑3 (MQ=255) gaaaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:2563564/70‑3 (MQ=255) aaaaTTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTa > 1:5446262/1‑71 (MQ=255) aaaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGttc < 1:212265/66‑2 (MQ=255) aaTTCCTCGACGAACGTGGGATCGTAGTAGAGaaa < 1:1631540/35‑1 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCAtc > 1:3633037/1‑70 (MQ=255) aTTCCTCGACGAACGTGGGATCGTAGTAGAGaaaa > 1:310909/1‑35 (MQ=255) ttCCTCGACGAACGTGGGATCGTAGTAGAGAAAAc > 1:4916474/1‑35 (MQ=255) ttCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTgg > 1:705990/1‑71 (MQ=255) taccTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCgg < 1:164118/69‑1 (MQ=255) cTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTAt > 1:880512/1‑71 (MQ=255) cgacgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTAt < 1:973463/63‑1 (MQ=255) cgacgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATc > 1:682283/1‑70 (MQ=255) gacgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAAc > 1:5039417/1‑42 (MQ=255) gacgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAAc > 1:2433211/1‑42 (MQ=255) gacgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATc < 1:4136023/69‑1 (MQ=255) gacgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATc < 1:288101/69‑1 (MQ=255) acgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGa > 1:3479110/1‑70 (MQ=255) acgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt < 1:3428638/71‑1 (MQ=255) acgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt < 1:2344690/71‑1 (MQ=255) cgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTtata < 1:3088921/38‑1 (MQ=255) gaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATa > 1:4932308/1‑70 (MQ=255) gaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCAtc > 1:1705181/1‑59 (MQ=12) aaCATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACctgc < 1:1434562/42‑1 (MQ=12) aCGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACctgc > 1:176999/1‑41 (MQ=255) aCGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGc > 1:4708487/1‑61 (MQ=255) | AATATGAGCGAGGAGGGGATCCCGGCGGCGCTGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATA > Exported/190010‑190130 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |