| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 834,548 | A→G | 38.2% | A1002A (GCA→GCG) | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 834,548 | 0 | A | G | 38.2% | 44.3 / 66.0 | 68 | A1002A (GCA→GCG) | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (20/22); new base G (15/11); total (35/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01 | |||||||||||
GGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCG > Exported/834480‑834618 | ggTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGcaca < 1:1678312/71‑1 (MQ=255) aacATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCcgcg > 1:5178234/3‑70 (MQ=255) acATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGc > 1:2943682/2‑65 (MQ=255) acATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGc > 1:650790/2‑65 (MQ=255) acATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCcgcgt > 1:4818750/2‑69 (MQ=255) acATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCcgcgt > 1:363581/2‑69 (MQ=255) acATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCcgcgt > 1:1830055/2‑69 (MQ=255) cATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCaa < 1:5646884/71‑1 (MQ=255) cATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTaa < 1:4857332/71‑1 (MQ=255) cATACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTaa < 1:4841285/71‑1 (MQ=255) tACCCATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTaaaa > 1:4450702/1‑71 (MQ=255) cccATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAAtt < 1:1796395/69‑1 (MQ=255) cGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCcgc > 1:1926493/1‑63 (MQ=255) ccATCTGGCGCTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTa < 1:1309694/70‑1 (MQ=255) cgcTGCACAAAGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAAtt < 1:4708290/62‑1 (MQ=255) gAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGa > 1:2512401/1‑71 (MQ=255) cacaaaGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCAc > 1:2355412/4‑71 (MQ=255) aaTAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCg < 1:1233782/69‑1 (MQ=255) aaTAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGa < 1:4425568/70‑1 (MQ=255) acaaaGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCAcc < 1:5475291/69‑1 (MQ=255) tAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCcgc > 1:632745/1‑47 (MQ=255) aaaGAGGACGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTAtc > 1:3525364/1‑57 (MQ=255) aagaagACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCaaaa > 1:2592900/1‑51 (MQ=255) aagaagACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCaaaa > 1:3180791/1‑51 (MQ=255) agaggaCGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCACctggt > 1:1276809/1‑71 (MQ=255) gaggaCGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCACctggtc < 1:2587385/68‑1 (MQ=255) gaagaCGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCaaaa < 1:2206360/49‑1 (MQ=255) gaagaCGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctg > 1:1353679/1‑68 (MQ=255) gaagaCGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACct > 1:5152866/1‑67 (MQ=255) aagaCGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctggtct > 1:3823411/1‑71 (MQ=255) gaCGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCcgc > 1:5619892/1‑40 (MQ=37) gaCGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGccc < 1:4885784/59‑1 (MQ=255) aCGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCACctggtccggt < 1:4856072/71‑1 (MQ=255) aCGAGAAGATTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCACctggtccggt < 1:1970290/71‑1 (MQ=255) aCGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctggtctggt > 1:3877035/1‑71 (MQ=255) cGAGAAGATCCGCTTCCGCGATATTCAGGCACAGcc < 1:2288202/36‑1 (MQ=255) cGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAg > 1:3621826/1‑54 (MQ=255) cGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCtggtctggtc < 1:237431/71‑1 (MQ=255) cGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCtggtctggtc < 1:3241924/71‑1 (MQ=255) gagaAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctggtctgg < 1:2699864/68‑1 (MQ=255) aagaTCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTAtctc < 1:3372584/47‑1 (MQ=255) agaTCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGtctggtctgg < 1:1168819/69‑1 (MQ=255) gaTTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCAc > 1:2737495/1‑54 (MQ=255) gaTTCGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCACCTGGTCCGGTCTGGaaa < 1:3602679/71‑2 (MQ=255) aTCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGtctcgtctgg < 1:728429/67‑1 (MQ=255) tCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAAt < 1:3432805/37‑1 (MQ=255) tCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTAt > 1:555055/1‑40 (MQ=255) tCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTAt < 1:5161902/40‑1 (MQ=255) tCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGAt < 1:2317952/71‑1 (MQ=255) tcGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCACCTGGTCCGGTCTGGaaag > 1:4281304/2‑67 (MQ=255) tcGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCACCTGGTCCGGTCTGGaaag > 1:4075744/2‑67 (MQ=255) tcGCTTTCGCGATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGCCCCACCTGGTCCGGTCTGGaaag > 1:445746/2‑67 (MQ=255) tcgcgATATTCAGGCGCAGCCGCGTAAAATTATCTCCAGcccc < 1:30116/42‑2 (MQ=37) cgcgATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACg < 1:1001419/70‑1 (MQ=255) cgcgATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGt > 1:4343050/1‑71 (MQ=255) cgcgATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGt > 1:4414918/1‑71 (MQ=255) gATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTc < 1:1515092/71‑1 (MQ=255) atatTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACctggtc < 1:3785735/46‑1 (MQ=255) atatTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCt < 1:4720274/71‑1 (MQ=255) ttCAGGCACAGCCGCGCAAAATTATCTCCAGCCCg < 1:4831188/35‑1 (MQ=255) ttCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTa > 1:3980592/1‑70 (MQ=255) cAGGCACAGCCGCGCAAAATTATCTCCAGCCCGAc > 1:1180945/1‑35 (MQ=255) cAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCtggtctggtc > 1:562999/1‑46 (MQ=255) cAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACaa > 1:913106/1‑71 (MQ=255) cAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACa < 1:3276936/70‑1 (MQ=255) cAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACa < 1:19916/70‑1 (MQ=255) aGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTggtctggtct > 1:4521034/1‑46 (MQ=255) aGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGgtctggtctg > 1:4440270/1‑47 (MQ=255) gCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGAt > 1:3828071/1‑51 (MQ=255) cacaGCCGCGCAAAATTATCTCCAGCCCGACctggtc > 1:548338/1‑37 (MQ=255) cacaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGcc > 1:5485810/1‑71 (MQ=255) acaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCg > 1:3431265/1‑71 (MQ=255) acaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCg > 1:2810182/1‑71 (MQ=255) acaGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCg > 1:775879/1‑71 (MQ=255) | GGTCGTGACCATACGCATCTGGCGCTGAATAAAGAAGACGAGAAGATCCGCTTCCGCGATATTCAGGCACAGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCG > Exported/834480‑834618 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |