Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 834,620 T→A 16.7% G1026G (GGT→GGA narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported834,6200TA16.7% 225.4 / 37.4 96G1026G (GGT→GGAnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (44/36);  new base A (9/7);  total (53/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

Only 100 of 101 total aligned reads displayed.

AGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACC  >  Exported/834550‑834690
                                                                      |                                                                      
aGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGt                                                                        <  1:3199465/71‑1 (MQ=255)
aGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGt                                                                        <  1:4438733/71‑1 (MQ=255)
      gcAAAATTATCTCCAGCCCGCCCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTacac                                                                   >  1:1316838/1‑70 (MQ=255)
      gcAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTacac                                                                   >  1:4136311/1‑70 (MQ=255)
       taaaaTTATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACAcc                                                                  >  1:2529333/2‑70 (MQ=255)
         aaaTTATCTCCAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAAc                                                               >  1:692219/1‑71 (MQ=255)
          aaTTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACg                                                              >  1:5147618/1‑71 (MQ=255)
            ttATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGtt                                                            <  1:4566101/71‑1 (MQ=255)
               tctcCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCAc                                                         >  1:3109015/1‑71 (MQ=255)
               tctcCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCAc                                                         >  1:422689/1‑71 (MQ=255)
                   cAGCCCGACCTGGTCTGGTCTGGAAGATGAACATGTTTCTTACAACGCCGGTTACACCAACGTTCAc                                                         <  1:3874263/67‑1 (MQ=255)
                   cAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAga                                                     <  1:3325402/71‑2 (MQ=255)
                   cAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGc                                                     >  1:2279472/1‑71 (MQ=255)
                   cAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGc                                                     >  1:3771642/1‑71 (MQ=255)
                   cAGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAgt                                                     >  1:3245122/1‑70 (MQ=255)
                    aGCCCCACCTGGTCCGGTCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAgtt                                                    <  1:3154329/71‑3 (MQ=255)
                        cGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGa                                                       >  1:5564691/1‑64 (MQ=255)
                          aCCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATcc                                               >  1:3053275/1‑70 (MQ=255)
                           ccTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                                             <  1:2859978/71‑1 (MQ=255)
                            ctggtccggtCTGGAAAGCTATCACGTCTCCTATAACGCGGGATACACCAACGtt                                                            <  1:1773061/55‑1 (MQ=255)
                            ctggtccggtCTGGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGtt                                                            <  1:4670662/55‑1 (MQ=255)
                             tggtctggtcTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATg                                           >  1:4345503/1‑71 (MQ=255)
                               gtctggtctgGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTg                                                   <  1:4221275/61‑1 (MQ=255)
                               gtctggtctgGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGc                                         >  1:1666562/1‑71 (MQ=255)
                               gtccggtctgGAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTgg                                          <  1:4443603/70‑1 (MQ=255)
                                 ctggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCg                                        >  1:1296049/1‑70 (MQ=255)
                                 ccggtctggAAAGAGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGc                                         >  1:2991480/3‑69 (MQ=255)
                                  tggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                                             <  1:1082990/64‑1 (MQ=255)
                                  tggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                                             <  1:1249383/64‑1 (MQ=255)
                                  tggtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                                             <  1:1256267/64‑1 (MQ=255)
                                   ggtctggAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCAc                                     <  1:4005560/71‑1 (MQ=255)
                                    gtctggAAGATGAACACGTTTCTTACAACGCCGGTTaca                                                                    >  1:3717928/1‑39 (MQ=255)
                                    gtctggAAGATGAACACGTTTCTTACAACGCCGGTTaca                                                                    >  1:4015644/1‑39 (MQ=255)
                                    gtctggAAGATGAACACGTTTCTTACAACGCCGGTTa                                                                      >  1:1806213/1‑37 (MQ=255)
                                    gtctggAAGATGAACACGTTTCTTACAACGCCGGTTa                                                                      >  1:4715456/1‑37 (MQ=255)
                                    gtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGt                                       <  1:191628/68‑1 (MQ=255)
                                    gtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTAc                                     <  1:3967120/70‑1 (MQ=255)
                                    gtctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTAc                                     <  1:4367756/70‑1 (MQ=255)
                                    gtctggAAAGCGATCACGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACg                                    >  1:4241321/1‑71 (MQ=255)
                                     tctggATGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTg                                                   <  1:3257601/55‑1 (MQ=255)
                                      ctggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGct                                  >  1:2064287/1‑71 (MQ=255)
                                       tggAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGc                                   <  1:4808438/69‑1 (MQ=255)
                                         gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctctc                               <  1:1764458/71‑1 (MQ=255)
                                         gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctctc                               >  1:5173350/1‑71 (MQ=255)
                                         gAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctctc                               <  1:1157887/71‑1 (MQ=255)
                                           aGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg                             <  1:1013436/71‑1 (MQ=255)
                                            gATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATcc                                               <  1:5523829/52‑1 (MQ=255)
                                              tGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctcac                               >  1:223304/1‑64 (MQ=255)
                                               gAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATcc                                               <  1:4209999/49‑1 (MQ=255)
                                               gAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcg                         >  1:1469327/1‑71 (MQ=255)
                                                aaCACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt                        <  1:2843237/71‑1 (MQ=255)
                                                 acacGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                                             <  1:2550197/49‑1 (MQ=255)
                                                 acacGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                                             <  1:2962516/49‑1 (MQ=255)
                                                 acacGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgtcgt                        >  1:4333227/1‑70 (MQ=255)
                                                   acGTTTCTTACAACGCCGGTTACACCAACGTTCACGAg                                                      >  1:4555694/1‑38 (MQ=255)
                                                   acGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAg                     >  1:1235246/1‑71 (MQ=255)
                                                   acGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAg                     >  1:5009384/1‑71 (MQ=255)
                                                   acGTCTCCTATAACGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAg                     <  1:266260/71‑1 (MQ=255)
                                                    cGTTTCTTACAACGCCGGTTACACCAACGTTCACGAg                                                      >  1:178022/1‑37 (MQ=255)
                                                    cGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAg                     >  1:1435995/1‑70 (MQ=255)
                                                    cGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGc                    <  1:2199704/71‑1 (MQ=255)
                                                      tttCTTACAACGCCGGTTACACCAACTTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGc                    <  1:135404/69‑1 (MQ=255)
                                                      tttCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATgg                                          <  1:3958168/47‑1 (MQ=255)
                                                      tttCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCaa                  >  1:538620/1‑71 (MQ=255)
                                                       ttCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATc                                                >  1:1707135/1‑40 (MQ=255)
                                                       ttCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGc                    >  1:2621404/1‑68 (MQ=255)
                                                       ttCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGc                    >  1:4392780/1‑68 (MQ=255)
                                                       ttCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGc                    >  1:814090/1‑68 (MQ=255)
                                                       ttCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGc                    >  1:2614700/1‑68 (MQ=255)
                                                        tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                                             >  1:174100/1‑42 (MQ=255)
                                                        tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                                             >  1:3060372/1‑42 (MQ=255)
                                                        tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGc                                   >  1:5035308/1‑52 (MQ=255)
                                                        tCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACt                >  1:1481969/1‑71 (MQ=255)
                                                         cTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgt                           >  1:3420004/1‑59 (MQ=255)
                                                         cTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGgt                           >  1:2537173/1‑59 (MQ=255)
                                                          ttACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg                             >  1:1369880/1‑56 (MQ=255)
                                                          ttACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg                             >  1:2963754/1‑56 (MQ=255)
                                                          ttACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg                             >  1:1440838/1‑56 (MQ=255)
                                                          ttACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTg                             >  1:863518/1‑56 (MQ=255)
                                                           tACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCa                                             <  1:1398375/39‑1 (MQ=255)
                                                            ataaCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTAt            >  1:924770/3‑71 (MQ=255)
                                                             taaCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTATc           <  1:4506961/70‑1 (MQ=255)
                                                             cAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGc                                         >  1:1838750/1‑41 (MQ=255)
                                                             cAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGc                                         >  1:2665952/1‑41 (MQ=255)
                                                             cAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATc           <  1:3411846/71‑1 (MQ=255)
                                                              aaCGCGGGATACACCAACGTTCATGAGTTAATTCCGTGGCGCACGCTGTCGGGACGCCAGCAGCTCTAt            >  1:3529316/1‑69 (MQ=255)
                                                              aaCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTAt            >  1:2246712/1‑69 (MQ=255)
                                                              aaCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTAt            >  1:3079501/1‑69 (MQ=255)
                                                              aaCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCa          <  1:5049300/71‑1 (MQ=255)
                                                              aaCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCa          <  1:1352667/71‑1 (MQ=255)
                                                              aaCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCa          <  1:615685/71‑1 (MQ=255)
                                                              aaCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCa          <  1:3459054/71‑1 (MQ=255)
                                                               aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctc                                 <  1:5262474/47‑1 (MQ=255)
                                                               aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGctc                                 <  1:4091012/47‑1 (MQ=255)
                                                               aCGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCa          <  1:5645872/70‑1 (MQ=255)
                                                                 gCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGa       <  1:4554497/71‑1 (MQ=255)
                                                                   cGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATc     <  1:1855271/71‑1 (MQ=255)
                                                                      ttACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAg         <  1:2815240/64‑1 (MQ=255)
                                                                      ttACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAcc  <  1:1293817/71‑1 (MQ=255)
                                                                      ttACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCAcc  <  1:3237351/71‑1 (MQ=255)
                                                                      |                                                                      
AGCCGCGCAAAATTATCTCCAGCCCGACCTGGTCTGGTCTGGAAGATGAACACGTTTCTTACAACGCCGGTTACACCAACGTTCACGAGCTGATCCCATGGCGTACGCTCTCTGGTCGTCAGCAACTGTATCAGGATCACC  >  Exported/834550‑834690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: