| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,614,466 | G→A | 16.7% | intergenic (‑91/+126) | eutH ← / ← eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,614,466 | 0 | G | A | 16.7% | 119.9 / 14.8 | 48 | intergenic (‑91/+126) | eutH/eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Reads supporting (aligned to +/- strand): ref base G (17/23); new base A (3/5); total (20/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01 | |||||||||||
TTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTA > Exported/1614398‑1614530 | ttatttatTGGCGGATGCGTCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGAc < 1:3870269/71‑1 (MQ=255) tatttattGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGAc < 1:4947492/70‑1 (MQ=255) gcggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCAt > 1:413232/1‑71 (MQ=39) cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATc > 1:1548697/1‑71 (MQ=39) cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATc > 1:4821866/1‑71 (MQ=39) cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATc > 1:503915/1‑71 (MQ=39) cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATc > 1:5023755/1‑71 (MQ=39) gTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGc < 1:1030584/71‑1 (MQ=39) gTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGc < 1:495362/71‑1 (MQ=39) aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:270339/1‑71 (MQ=38) aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:3753546/1‑71 (MQ=38) gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc < 1:638910/71‑1 (MQ=255) gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc < 1:4344863/71‑1 (MQ=255) gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc < 1:400448/71‑1 (MQ=255) gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc < 1:3494483/71‑1 (MQ=255) cTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGc < 1:645112/69‑1 (MQ=11) cTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGc < 1:284218/69‑1 (MQ=11) cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc < 1:1768806/71‑1 (MQ=255) cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc > 1:677866/1‑71 (MQ=255) cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc < 1:3801480/71‑1 (MQ=255) cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc < 1:3337926/71‑1 (MQ=255) tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:4874531/46‑1 (MQ=38) tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:4770734/46‑1 (MQ=38) tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:1305743/46‑1 (MQ=38) tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:3583129/46‑1 (MQ=38) tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:1698984/46‑1 (MQ=38) tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:2677118/46‑1 (MQ=38) tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:2228795/46‑1 (MQ=38) tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:24955/46‑1 (MQ=38) tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:2256118/46‑1 (MQ=38) aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcgc > 1:1454422/1‑71 (MQ=255) aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcg > 1:4521302/1‑70 (MQ=255) tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAg > 1:3216677/1‑46 (MQ=38) tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAg > 1:2876117/1‑46 (MQ=38) tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAg > 1:2626482/1‑46 (MQ=38) tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAg > 1:2363660/1‑46 (MQ=38) tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAg > 1:2332232/1‑46 (MQ=38) accGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCaa > 1:5169620/2‑45 (MQ=38) cGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCgcgc < 1:1875160/70‑1 (MQ=255) aCATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGc > 1:3971104/1‑71 (MQ=255) cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg > 1:3481713/1‑71 (MQ=14) cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg > 1:2517284/1‑71 (MQ=14) cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg > 1:711761/1‑71 (MQ=14) cATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGc < 1:2539557/70‑1 (MQ=255) aGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAAc < 1:322710/70‑1 (MQ=255) ggATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTa < 1:4770001/71‑1 (MQ=18) ggATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTa < 1:2912512/71‑1 (MQ=18) ggATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTa < 1:863468/71‑1 (MQ=18) | TTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCGGATGCGGCGTGAACGCCTTA > Exported/1614398‑1614530 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |