Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,635,086 C→T 12.4% D408D (GAC→GAT acrD → aminoglycoside/multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,635,0860CT12.4% 474.4 / 39.3 153D408D (GAC→GATacrDaminoglycoside/multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base C (60/74);  new base T (13/6);  total (73/80)
Fisher's exact test for biased strand distribution p-value = 8.39e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

Only 100 of 153 total aligned reads displayed.

TCGGTTACAGCGTCAACACCTTAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGAGGAAGGACTCACTCCTCGCGA  >  Exported/1635019‑1635148
                                                                   |                                                              
tCGGTTACAGCGTCAACACCTTAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGc                                                              >  1:2748444/1‑70 (MQ=255)
 cGGTTACAGCGTCAACACCTTAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGcc                                                             >  1:405231/1‑70 (MQ=255)
 cGGTTACAGCGTCAACACCTTAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCa                                                            >  1:1989986/1‑71 (MQ=255)
 cGGTTACAGCGTCAACACCTTAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCa                                                            >  1:3328386/1‑71 (MQ=255)
  ggTTACAGCGTCAACACCTTAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCAt                                                           >  1:947508/1‑71 (MQ=255)
      aCAGCGTCAACACCTTAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCgtg                                                       >  1:2393393/1‑71 (MQ=255)
      aCAGCGTCAACACCTTAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCgtg                                                       >  1:3890937/1‑71 (MQ=255)
      aCAGCGTCAACACCTTAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCgtg                                                       >  1:1568737/1‑71 (MQ=255)
      aCAGCGTCAACACCTTAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCgtg                                                       >  1:2982639/1‑71 (MQ=255)
          cGTCAACACCTTAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCgtgg                                                      >  1:4218691/1‑68 (MQ=255)
          cGTCAACACCTTAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCgtgg                                                      >  1:1136134/1‑68 (MQ=255)
          cGTCAACACCTTAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCgtgg                                                      >  1:3059938/1‑68 (MQ=255)
          cGTCAACACCTTAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCgtgg                                                      >  1:2966588/1‑68 (MQ=255)
          cGTCAACACCTTAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCgtgg                                                      >  1:1093926/1‑68 (MQ=255)
                  ccTTAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAAc                                           <  1:4979862/71‑1 (MQ=255)
                  ccTTAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAAc                                           <  1:709375/71‑1 (MQ=255)
                   cTTAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACg                                          <  1:4597132/71‑1 (MQ=255)
                    ttAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACg                                          <  1:1744812/70‑1 (MQ=255)
                    ttAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGt                                         >  1:2678589/1‑71 (MQ=255)
                    ttAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGt                                         >  1:2765145/1‑71 (MQ=255)
                      aaCCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTc                                        >  1:221541/1‑70 (MQ=255)
                      aaCCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTc                                        >  1:3126186/1‑70 (MQ=255)
                      aaCCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTc                                        >  1:5038318/1‑70 (MQ=255)
                       aCCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCg                                       <  1:1062883/70‑1 (MQ=255)
                       aCCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCg                                       <  1:1833506/70‑1 (MQ=255)
                       aCCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCg                                       <  1:1341214/70‑1 (MQ=255)
                         cATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAAc                                    >  1:1277644/1‑71 (MQ=255)
                         cATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAAc                                    >  1:4166605/1‑71 (MQ=255)
                          aTGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACg                                   <  1:306759/71‑1 (MQ=255)
                           tGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGt                                  <  1:2890152/71‑1 (MQ=255)
                           tGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGt                                  <  1:1875104/71‑1 (MQ=255)
                           tGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGt                                  <  1:3279448/71‑1 (MQ=255)
                             ttCGCGATGGTGCTGGCGATCGGTCTGCTGGTTGATGACGCCATCgtggtg                                                    >  1:4367078/1‑51 (MQ=255)
                             ttCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCgtggtg                                                    >  1:3229917/1‑51 (MQ=255)
                             ttCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCgtggtg                                                    >  1:1382098/1‑51 (MQ=255)
                             ttCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCgtggtg                                                    >  1:93805/1‑51 (MQ=255)
                             ttCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGtat                                <  1:211187/71‑1 (MQ=255)
                             ttCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGtat                                <  1:1822472/71‑1 (MQ=255)
                             ttCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGta                                 <  1:4265750/70‑1 (MQ=255)
                             ttCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGta                                 <  1:4846325/70‑1 (MQ=255)
                               cgcgATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCgtggtggt                                                  >  1:3684706/1‑51 (MQ=255)
                               cgcgATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCgtggtggt                                                  >  1:1030493/1‑51 (MQ=255)
                               cgcgATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCgtggtggt                                                  >  1:79819/1‑51 (MQ=255)
                               cgcgATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGtatta                              >  1:2117281/1‑71 (MQ=255)
                                 cgATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGtattat                             >  1:2447117/1‑70 (MQ=255)
                                 cgATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTAtg                            <  1:4755224/71‑1 (MQ=255)
                                 cgATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTAtg                            <  1:1702836/71‑1 (MQ=255)
                                 cgATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTAtg                            <  1:507452/71‑1 (MQ=255)
                                 cgATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTAtg                            <  1:658019/71‑1 (MQ=255)
                                 cgATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTAtg                            <  1:819414/71‑1 (MQ=255)
                                 cgATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTAtg                            <  1:3403206/71‑1 (MQ=255)
                                 cgATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTAtg                            <  1:3484587/71‑1 (MQ=255)
                                  gATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTAtg                            <  1:3704995/70‑1 (MQ=255)
                                    tGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGtat                                <  1:119053/64‑1 (MQ=255)
                                    tGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGtat                                <  1:4077215/64‑1 (MQ=255)
                                    tGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGtat                                <  1:3581441/64‑1 (MQ=255)
                                      gTGCTGGTGATCGGTATCGTGGTCGACGATGCGATCGTGGTGGTCGAGAACGTCGAGCGCATCAtgagcg                        <  1:3776631/70‑3 (MQ=2)
                                      gTGCTGGTGATCGGTATCGTGGTCGACGATGCGATCGTGGTGGTCGAGAACGTCGAGCGCATCAtgagcg                        <  1:128212/70‑3 (MQ=2)
                                      gTGCTGGTGATCGGTATCGTGGTCGACGATGCGATCGTGGTGGTCGAGAACGTCGAGCGCATCAtgagcg                        <  1:4455651/70‑3 (MQ=2)
                                      gTGCTGGTGATCGGTATCGTGGTCGACGATGCGATCGTGGTGGTCGAGAACGTCGAGCGCATCAtgagcg                        <  1:3673076/70‑3 (MQ=2)
                                        gCTGGCGATCGGCTTGCTGGTGGACGATGCCATTGTGGTGGTAGAGAACGTCGaa                                     >  1:4916232/1‑55 (MQ=2)
                                        gCTGGCGATCGGCTTGCTGGTGGACGATGCCATTGTGGTGGTAGAGAACGTCGaa                                     >  1:5084635/1‑55 (MQ=2)
                                        gCTGGCGATCGGCTTGCTGGTGGACGATGCCATTGTGGTGGTAGAGAACGTCGaa                                     >  1:2969704/1‑55 (MQ=2)
                                        gCTGGCGATCGGCTTGCTGGTGGACGATGCCATTGTGGTGGTAGAGAACGTCGaa                                     >  1:3000726/1‑55 (MQ=2)
                                        gCTGGCGATCGGCTTGCTGGTGGACGATGCCATTGTGGTGGTAGAGAACGTCGaa                                     >  1:4048464/1‑55 (MQ=2)
                                        gCTGGCGATCGGCTTGCTGGTGGACGATGCCATTGTGGTGGTAGAGAACGTCGaa                                     >  1:117725/1‑55 (MQ=2)
                                        gCTGGCGATCGGCTTGCTGGTGGACGATGCCATTGTGGTGGTAGAGAACGTCGaa                                     >  1:3047551/1‑55 (MQ=2)
                                        gCTGGCGATCGGCTTGCTGGTGGACGATGCCATTGTGGTGGTAGAGAACGTCGaa                                     >  1:4184965/1‑55 (MQ=2)
                                        gCTGGCGATCGGCTTGCTGGTGGACGATGCCATTGTGGTGGTAGAGAACGTCGaa                                     >  1:4287499/1‑55 (MQ=2)
                                         cTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGagga                    <  1:560793/71‑1 (MQ=255)
                                          tGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGtat                                <  1:4285852/58‑1 (MQ=255)
                                               aTCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGaggaag                  <  1:3093573/67‑1 (MQ=255)
                                               aTCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGaggaag                  <  1:565827/67‑1 (MQ=255)
                                               aTCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGaggaag                  <  1:2330594/67‑1 (MQ=255)
                                               aTCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGaggaag                  <  1:5175411/67‑1 (MQ=255)
                                                 cGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTAtgag                          >  1:882992/1‑57 (MQ=255)
                                                 cGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTAtgag                          >  1:2274638/1‑57 (MQ=255)
                                                  ggTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGAGGAAGGactcac           <  1:3111824/71‑1 (MQ=255)
                                                  ggTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGAGGAAGGactcac           <  1:2331877/71‑1 (MQ=255)
                                                   gTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGAGGAAGGactcact          >  1:3886201/1‑71 (MQ=255)
                                                   gTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGAGGAAGGactcact          >  1:241733/1‑71 (MQ=255)
                                                    tCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGAGGAAGGactcactc         <  1:1785846/71‑1 (MQ=255)
                                                     ctgctgGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGAGGAAGGACTCActcc        >  1:2367548/1‑71 (MQ=255)
                                                      tgctgGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTAtgagt                         >  1:487105/1‑53 (MQ=255)
                                                        ctgGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGAGGAAGGACTCACTCCTcg     >  1:5181078/1‑71 (MQ=255)
                                                           gtggATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGAGGAAGGACTCACTCCTcgc    <  1:3636201/69‑1 (MQ=255)
                                                           gtggATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGAGGAAGGACTCACTCCTcgc    <  1:4653341/69‑1 (MQ=255)
                                                           gtggATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGAGGAAGGACTCACTCCTcgc    <  1:3311896/69‑1 (MQ=255)
                                                           gtggATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGAGGAAGGACTCACTCCTcgc    <  1:2203629/69‑1 (MQ=255)
                                                           gtggATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGAGGAAGGACTCACTCCTCGCGa  <  1:1470959/71‑1 (MQ=255)
                                                                  aCGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGagga                    <  1:2593864/46‑1 (MQ=255)
                                                                  aCGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGagga                    <  1:2373444/46‑1 (MQ=255)
                                                                  aCGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGagga                    <  1:2874611/46‑1 (MQ=255)
                                                                  aCGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGagga                    <  1:4668571/46‑1 (MQ=255)
                                                                  aCGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGagga                    <  1:4430847/46‑1 (MQ=255)
                                                                  aCGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGagga                    <  1:434284/46‑1 (MQ=255)
                                                                  aCGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGagga                    <  1:145996/46‑1 (MQ=255)
                                                                  aCGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGagga                    <  1:1421678/46‑1 (MQ=255)
                                                                  aCGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGagga                    <  1:3629139/46‑1 (MQ=255)
                                                                  aCGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGagga                    <  1:2196790/46‑1 (MQ=255)
                                                                   |                                                              
TCGGTTACAGCGTCAACACCTTAACCATGTTCGCGATGGTGCTGGCGATCGGTCTGCTGGTGGATGACGCCATCGTGGTGGTGGAAAACGTCGAACGTATTATGAGTGAGGAAGGACTCACTCCTCGCGA  >  Exported/1635019‑1635148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: