| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 86,479 | T→G | 25.0% | G325G (GGT→GGG) | murC → | UDP‑N‑acetylmuramate:L‑alanine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 86,479 | 0 | T | G | 25.0% | 35.1 / 21.3 | 32 | G325G (GGT→GGG) | murC | UDP‑N‑acetylmuramate:L‑alanine ligase |
| Reads supporting (aligned to +/- strand): ref base T (9/15); new base G (4/4); total (13/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01 | |||||||||||
GGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGAATGGTAAAAGCGG > Exported/86419‑86535 | ggAAGAGGGAATTGACGACGAGGCTATTTTGCGTGCGCTGGAGAGCTTCCAGGGGACAGGGCGCCGtttcg < 1:1109787/71‑3 (MQ=255)ggAAGAGGGAATTGACGACGAGGCTATTTTGCGTGCGCTGGAGAGCTTCCAGGGGACAGGGCGCCGtttcg < 1:4036301/71‑3 (MQ=255)ggAAGAGGGAATTGACGACGAGGCTATTTTGCGTGCGCTGGAGAGCTTCCAGGGGACAGGGCGCCGtttcg < 1:3517735/71‑3 (MQ=255) gAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGa < 1:1478463/71‑1 (MQ=255) agagGGAATTGACGACGAGGCTATTTTGCGTGCGCTGGAGAGCTTCCAGGGGACAGGGCGCCGtttcg < 1:3610141/68‑3 (MQ=255) gggCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTcc < 1:3597199/71‑1 (MQ=255) ggCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCt > 1:4067191/1‑71 (MQ=255) gacgacGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGt > 1:4124425/1‑48 (MQ=255) gacgacGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTc > 1:3575756/1‑49 (MQ=255) acgaGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTg < 1:722848/66‑1 (MQ=255) acgaGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTg < 1:4053289/66‑1 (MQ=255) acgaGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTg < 1:2298590/66‑1 (MQ=255) gaGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTccc < 1:1927492/71‑1 (MQ=255) gaGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTccc < 1:2249618/71‑1 (MQ=255) gCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTg < 1:1131740/61‑1 (MQ=255) gCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTg < 1:842315/61‑1 (MQ=255) tttGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGaa > 1:4175009/1‑58 (MQ=255) ttGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCg > 1:3176349/1‑35 (MQ=255) ttGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGTTTTCCTCg > 1:5044937/1‑52 (MQ=255) ttGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCt > 1:2387140/1‑65 (MQ=255) ttGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGc > 1:3874898/1‑70 (MQ=255) tGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGc > 1:1437559/1‑69 (MQ=255) gagaGCTTCCAGGGGACAGGGCGCCGTTTCGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGAATGGTa > 1:707271/4‑70 (MQ=255) gagaGCTTCCAGGGGACAGGGCGCCGTTTCGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGAATGGTa > 1:1797043/4‑70 (MQ=255) gagaGCTTCCAGGGGACAGGGCGCCGTTTCGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGAATGGTa > 1:12814/4‑70 (MQ=255) gagaGCTTCCAGGGGACAGGGCGCCGTTTCGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGAATGGTa > 1:4165730/4‑70 (MQ=255) ttCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTg < 1:2305264/37‑1 (MQ=255) tCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGAATGGTAAAAGCgg > 1:4153361/1‑70 (MQ=255) tGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGaa < 1:2657563/48‑1 (MQ=255) tGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGaa < 1:1189819/48‑1 (MQ=255) tGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGaa < 1:1242226/48‑1 (MQ=255) tGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGaa < 1:3932359/48‑1 (MQ=255) tGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGaa < 1:3621056/48‑1 (MQ=255) tCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGAATGGTAAAAg < 1:2112260/54‑1 (MQ=255) tCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGAATGGTAAAAg < 1:845347/54‑1 (MQ=255) tCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGAATGCTAAAAg < 1:662526/54‑1 (MQ=255) | GGAAGAGGGCATTGACGACGAGGCTATTTTGCGGGCGCTTGAAAGCTTCCAGGGGACTGGTCGCCGTTTTGATTTCCTCGGTGAATTCCCGCTGGAGCCAGTGAATGGTAAAAGCGG > Exported/86419‑86535 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |