| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,768,269 | A→G | 22.4% | T143T (ACT→ACC) | rluD ← | 23S rRNA pseudouridine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,768,269 | 0 | A | G | 22.4% | 108.8 / 29.3 | 58 | T143T (ACT→ACC) | rluD | 23S rRNA pseudouridine synthase |
| Reads supporting (aligned to +/- strand): ref base A (19/26); new base G (9/4); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.80e-01 | |||||||||||
TCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAA > Exported/1768201‑1768335 | tCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAgt > 1:1287926/1‑71 (MQ=255) cGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTc < 1:4881670/71‑1 (MQ=255) gCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCtt > 1:3805316/1‑69 (MQ=255) gCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCtt > 1:609592/1‑69 (MQ=255) cAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTAt < 1:62579/71‑1 (MQ=255) aaaGATTCGACTAAACGCGGCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATc < 1:856390/71‑1 (MQ=255) aaGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATc > 1:4756628/1‑70 (MQ=255) aaGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATc > 1:4937276/1‑70 (MQ=255) aGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAg < 1:695043/56‑1 (MQ=255) ttCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAgac < 1:2475481/71‑1 (MQ=255) cGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGCTGTCTTTATCCAgacg < 1:1077922/70‑1 (MQ=255) cTAACCGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAgacgatg > 1:394098/1‑70 (MQ=255) cTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAgacgatg > 1:333370/1‑70 (MQ=255) aaaCGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAgacgatggac > 1:1031982/1‑71 (MQ=255) aaaCGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAgacg < 1:4671128/65‑1 (MQ=255) aaCGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCGGTGGTGTCTTTATCCAgacgatggacg < 1:5192736/71‑1 (MQ=255) aaCGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCGGTGGTGTCTTTATCCAgacgatggacg < 1:668881/71‑1 (MQ=255) aaCGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCGGTGGTGTCTTTATCCAgacgatggacg < 1:3954815/71‑1 (MQ=255) aaCGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCGGTGGTGTCTTTATCCAgacgatggacg < 1:674918/71‑1 (MQ=255) tGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCa > 1:3711781/1‑52 (MQ=255) tGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCa > 1:3285407/1‑52 (MQ=255) tGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCa > 1:4954626/1‑52 (MQ=255) tGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCa > 1:895989/1‑52 (MQ=255) tGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCa > 1:1172230/1‑52 (MQ=255) tGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCa > 1:1234699/1‑52 (MQ=255) tGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCa > 1:1385071/1‑52 (MQ=255) tGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCa > 1:223549/1‑52 (MQ=255) tGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAgac < 1:2293377/55‑1 (MQ=255) aGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCg < 1:1183548/68‑1 (MQ=255) aGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCg < 1:3557312/68‑1 (MQ=255) aGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCg < 1:1714990/68‑1 (MQ=255) aGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCg < 1:4388085/68‑1 (MQ=255) aGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCg < 1:2214542/68‑1 (MQ=255) gCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCg < 1:4525035/67‑1 (MQ=255) ccGGAACGGTTTTTGCCACAACCATCAGGCCGGTGGTGTc > 1:212814/1‑40 (MQ=255) ccGGAACGGTTTTTGCCACAACCATCAGGCCGGTGGTGTc > 1:953618/1‑40 (MQ=255) ccGGAACGGTTTTTGCCACAACCATCAGGCCGGTGGTGTc > 1:1875520/1‑40 (MQ=255) ccGGAACGGTTTTTGCCACAACCATCAGGCCGGTGGTGTc > 1:4935562/1‑40 (MQ=255) ccGGAACGGTTTTTGCCACAACCATCAGGCCGGTGGTGTc > 1:4920191/1‑40 (MQ=255) ccGGAACGGTTTTTGCCACAACCATCAGGCCGGTGGTGTc > 1:4475045/1‑40 (MQ=255) ccGGAACGGTTTTTGCCACAACCATCAGGCCGGTGGTGTc > 1:4067852/1‑40 (MQ=255) ccGGAACGGTTTTTGCCACAACCATCAGGCCGGTGGTGTc > 1:2440229/1‑40 (MQ=255) ccGGAACGGTTTTTGCCACAACCATCAGGCCGGTGGTGTc > 1:2336165/1‑40 (MQ=255) cGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCAcgc < 1:2866581/70‑1 (MQ=255) ttGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGc < 1:1995938/70‑1 (MQ=255) gCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAAt > 1:2989975/1‑71 (MQ=255) gCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAAt > 1:3196140/1‑71 (MQ=255) aaCCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTg < 1:1260436/71‑1 (MQ=255) aaCCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTg < 1:697998/71‑1 (MQ=255) aCCATCAGGCCATTTGTGTCTTTATCCAgacgatggac < 1:376026/38‑1 (MQ=37) aCCATCAGGCCAGTTGTGTCTTTATCCAgacgatggac < 1:1920200/38‑1 (MQ=255) aCCATCAGGCCAGTGGTGTCTTTATCCAgacgatggac < 1:1684132/38‑1 (MQ=255) aCCATCAGGCCAGTGGTGTCTTTATCCAgacgatggac < 1:784046/38‑1 (MQ=255) aCCATCAGGCCAGTGGTGTCTTTATCCAgacgatggac < 1:452299/38‑1 (MQ=255) aCCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTg < 1:2327571/70‑1 (MQ=255) tCAGGCCAGTGGTGTCTTTATCCAgacgatggacgat < 1:4966811/37‑1 (MQ=255) aGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGt < 1:3214185/70‑1 (MQ=255) gCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTaa > 1:1635730/1‑70 (MQ=255) | TCACGCCGTTGCAAAGATTCGACTAAACGCGTCTGAGCCGGAACGGTTTTTGCCACAACCATCAGGCCAGTGGTGTCTTTATCCAGACGATGGACGATGCCCGCACGCGGTACATCGGCAATGGGTGGATAGTAA > Exported/1768201‑1768335 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |