| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,844,947 | A→G | 8.5% | R46R (CGT→CGC) | cysN ← | sulfate adenylyltransferase, subunit 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,844,947 | 0 | A | G | 8.5% | 371.0 / 18.8 | 129 | R46R (CGT→CGC) | cysN | sulfate adenylyltransferase, subunit 1 |
| Reads supporting (aligned to +/- strand): ref base A (52/66); new base G (5/6); total (57/72) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
Only 100 of 131 total aligned reads displayed.
ACGCTTACTGTCGTTATGCAGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTACCACAGGTCAGAAAACGCAGCAGGCTTTTAT > Exported/1844881‑1845014 | aCGCTTACTGTCGTTATGCAGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAgacgac > 1:3736415/1‑71 (MQ=255) gCTTACTGTCGTTATGCAGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGAcc > 1:141990/1‑70 (MQ=255) cTGTCGTTATGCAGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCa < 1:2810687/70‑1 (MQ=255) cTGTCGTTATGCAGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCa < 1:3207204/70‑1 (MQ=255) cTGTCGTTATGCAGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCa < 1:4651229/70‑1 (MQ=255) cTGTCGTTATGCAGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCa < 1:4645114/70‑1 (MQ=255) cTGTCGTTATGCAGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCa < 1:4333932/70‑1 (MQ=255) cTGTCGTTATGCAGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCa < 1:4239191/70‑1 (MQ=255) cTGTCGTTATGCAGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCa < 1:1782674/70‑1 (MQ=255) cTGTCGTTATGCAGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCa < 1:1657927/70‑1 (MQ=255) cTGTCGTTATGCAGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCa < 1:4214054/70‑1 (MQ=255) tCGTTATGCAGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCagag > 1:3131617/1‑70 (MQ=255) tCGTTATGCAGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCagag > 1:1544888/1‑70 (MQ=255) aTGCAGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACtt < 1:929970/70‑1 (MQ=255) aTGCAGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACtt < 1:2103348/70‑1 (MQ=255) aTGCAGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACtt < 1:3422146/70‑1 (MQ=255) aTGCAGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACtt < 1:4979159/70‑1 (MQ=255) aTGCAGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATAGTGCAGCAGACGACCAATCAGAGTACtt < 1:3468942/70‑1 (MQ=255) tGCAGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACtttt < 1:3735925/71‑1 (MQ=255) tGCAGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACtttt < 1:4224143/71‑1 (MQ=255) tGCAGCGATGACAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACttt < 1:4849246/70‑1 (MQ=255) aGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGcc > 1:1387113/1‑71 (MQ=255) gCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCg < 1:3530537/71‑1 (MQ=255) gCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCg < 1:3127250/71‑1 (MQ=255) gCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCg < 1:900538/71‑1 (MQ=255) gCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCg > 1:2317683/1‑71 (MQ=255) gCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCg > 1:2120284/1‑71 (MQ=255) gCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCg > 1:2199484/1‑71 (MQ=255) cGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCa < 1:1560608/56‑1 (MQ=255) cGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCa < 1:1257070/56‑1 (MQ=255) cGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCg < 1:1092429/70‑1 (MQ=255) gATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCagag > 1:3073457/1‑58 (MQ=255) tGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGGCGACCAATCAGGGTACTTTTGCCGTCAt > 1:4957516/1‑71 (MQ=255) tGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGGCGACCAATCAGGGTACTTTTGCCGTCAt > 1:1250219/1‑71 (MQ=255) tGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGGCGACCAATCAGGGTACTTTTGCCGTCAt > 1:599302/1‑71 (MQ=255) tGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGGCGACCAATCAGGGTACTTTTGCCGTCAt > 1:2819545/1‑71 (MQ=255) gagaGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACttt > 1:1749413/1‑61 (MQ=255) agagCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATc < 1:1014456/70‑1 (MQ=255) gagCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACGAATCAGAGTACTTTTGCCGTCATCGa < 1:4759899/71‑1 (MQ=255) gCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGGCGACCAATCAGGGTACTTTTGCCGTCATCGACg < 1:3536551/71‑1 (MQ=255) gCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGGCGACCAATCAGGGTACTTTTGCCGTCATCGACg < 1:1841782/71‑1 (MQ=255) gCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGGCGACCAATCAGGGTACTTTTGCCGTCATCGACg < 1:2805181/71‑1 (MQ=255) cTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGGCGACCAATCAGGGTACTTTTGCCGTCATCGACg < 1:4455371/70‑1 (MQ=255) tGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGc < 1:2104815/70‑1 (MQ=255) gATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATc < 1:4663480/64‑1 (MQ=255) gATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATc < 1:204410/64‑1 (MQ=255) gATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATc < 1:1168310/64‑1 (MQ=255) gATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATc < 1:1477745/64‑1 (MQ=255) gATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATc < 1:3250941/64‑1 (MQ=255) aTCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATc < 1:4515000/63‑1 (MQ=255) aTCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATc < 1:4083309/63‑1 (MQ=255) aTCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCt < 1:3101550/69‑1 (MQ=255) aTCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCt < 1:2609820/69‑1 (MQ=255) aTCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCt < 1:4120070/69‑1 (MQ=255) cTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACg > 1:4984116/1‑65 (MQ=255) cTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACg > 1:2640055/1‑65 (MQ=255) cTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACg > 1:5151043/1‑65 (MQ=255) cTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACg > 1:5156940/1‑65 (MQ=255) cTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACg > 1:3572549/1‑65 (MQ=255) ttCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATc < 1:2564054/60‑1 (MQ=255) ttCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATc < 1:4399680/60‑1 (MQ=255) cGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGa > 1:4754769/1‑60 (MQ=255) cGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGa > 1:795834/1‑60 (MQ=255) cGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGa > 1:2190321/1‑60 (MQ=255) cGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGa > 1:182027/1‑60 (MQ=255) gTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCagag < 1:5154085/41‑1 (MQ=255) gTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCagag < 1:3778932/41‑1 (MQ=255) gTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCagag < 1:913811/41‑1 (MQ=255) gATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTACCACAGGt > 1:1639488/1‑69 (MQ=255) gATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTACCACAGGt > 1:3487178/1‑69 (MQ=255) tttGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTACCACAGGTCAg < 1:1784122/70‑1 (MQ=255) tttGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTACCACAGGTCAg < 1:1656710/70‑1 (MQ=255) ttGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGa > 1:825490/1‑53 (MQ=255) ttGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGa > 1:1307302/1‑53 (MQ=255) ttGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGa > 1:1329625/1‑53 (MQ=255) ttGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGa > 1:5194300/1‑53 (MQ=255) ttGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGa > 1:3774012/1‑53 (MQ=255) ttGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTACCACAGGTCAGa > 1:4343903/1‑70 (MQ=255) ttGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTACCACAGGTCAGa > 1:698746/1‑70 (MQ=255) ttGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTACCACAGGTCAGa > 1:3495973/1‑70 (MQ=255) tGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTACCACAGGTCAGaa < 1:3812067/70‑1 (MQ=255) tGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTACCACAGGTCAGaa < 1:1829053/70‑1 (MQ=255) cGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTACCACAGGTCATAAAACg < 1:11128/71‑1 (MQ=255) cGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTACCACAGGTCAGAAAACg < 1:1103728/71‑1 (MQ=255) cGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTACCACAGGTCAGAAAACg < 1:1174046/71‑1 (MQ=255) gggTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTACCACAGGTCAGAAAACg > 1:506740/1‑70 (MQ=255) gggTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTACCACAGGTCAGAAAACg > 1:3429346/1‑70 (MQ=255) ggTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATc < 1:118449/45‑1 (MQ=255) gTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTACCACAGGTCAGAAAACgcag < 1:807625/71‑1 (MQ=255) gTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGACGTCATCGACGCTACCACAGGTCAGAAAACgcag < 1:3782357/71‑1 (MQ=255) aTCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCt < 1:3195889/48‑1 (MQ=255) tCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTAc < 1:3597245/49‑1 (MQ=255) tCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTAc < 1:2362473/49‑1 (MQ=255) tCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTAc < 1:1478242/49‑1 (MQ=255) gTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTACCACAGGTCa > 1:3432887/1‑56 (MQ=255) gTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTACCACAGGTCa > 1:2426314/1‑56 (MQ=255) gTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTACCACAGGTCa > 1:4356694/1‑56 (MQ=255) gTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTACCACAGGTCa > 1:2295246/1‑56 (MQ=255) gcagcaGGCGACCAATCAGGGTACTTTTGCCGTCATCGACg < 1:3627449/41‑1 (MQ=37) gacgacCAATCAGAGTACTTTTGCCGTCATCGACGCTACCACAGGTCAGAAAACGCAGCAGGCTTTTAt < 1:4600178/69‑1 (MQ=255) | ACGCTTACTGTCGTTATGCAGCGATGAGAGCTGATCTTCGTAGATTTGGCGGGTATCGTGCAGCAGACGACCAATCAGAGTACTTTTGCCGTCATCGACGCTACCACAGGTCAGAAAACGCAGCAGGCTTTTAT > Exported/1844881‑1845014 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |