BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	1,876,425	T→C	5.5%	K333K (AAA→AAG)	eno ←	enolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	1,876,425	0	T	C	5.5%	497.9 / 13.1	164	K333K (AAA→AAG)	eno	enolase
Reads supporting (aligned to +/- strand): ref base T (82/73); new base C (7/2); total (89/75)
Fisher's exact test for biased strand distribution p-value = 1.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

Only 100 of 172 total aligned reads displayed.

ATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTT  >  Exported/1876357‑1876493

aTTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCtt                                                                     <  1:231445/70‑1 (MQ=255)

aTTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCtt                                                                     <  1:507784/70‑1 (MQ=255)

    cAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:989675/70‑1 (MQ=255)

         agagTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATa                                                             >  1:4930577/1‑69 (MQ=255)

           agTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCg                                                                <  1:1080757/64‑1 (MQ=255)

           agTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCg                                                                <  1:3957510/64‑1 (MQ=255)

             tttCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCttt                                                                    <  1:1213083/58‑1 (MQ=255)

               tCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCttt                                                     >  1:947790/1‑71 (MQ=255)

                 ggTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTc                                                    <  1:480855/70‑1 (MQ=255)

                 ggTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTc                                                    <  1:1583553/70‑1 (MQ=255)

                     agagAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTc                                                    <  1:880171/66‑1 (MQ=255)

                     agagAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTc                                                    <  1:4624269/66‑1 (MQ=255)

                     agagAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTc                                                    <  1:2877634/66‑1 (MQ=255)

                       agaACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAg                                                  >  1:4981690/1‑66 (MQ=255)

                       agaACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAg                                                  >  1:2001127/1‑66 (MQ=255)

                       agaACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCt                                             >  1:2468298/1‑71 (MQ=255)

                         aaCCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:91402/49‑1 (MQ=255)

                         aaCCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:285375/49‑1 (MQ=255)

                         aaCCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:997366/49‑1 (MQ=255)

                         aaCCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAg                                                  >  1:4603730/1‑64 (MQ=255)

                           ccGATCTGGTTGAATTTGATAAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                         >  1:2914062/1‑71 (MQ=255)

                            cGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgtg                                        >  1:558299/1‑71 (MQ=255)

                              aTCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCttt                                                     <  1:3816641/56‑1 (MQ=255)

                              aTCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAgg                                                 <  1:3779344/60‑1 (MQ=255)

                              aTCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAgg                                                 <  1:3222279/60‑1 (MQ=255)

                               tCTGGTTTAATTTGATCAGTATGGAGTTAGCGATACCttt                                                                    <  1:4259852/40‑1 (MQ=38)

                                cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTc                                                                 <  1:794516/42‑1 (MQ=255)

                                cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCa                                                   >  1:3106115/1‑56 (MQ=255)

                                cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCa                                                   >  1:1802947/1‑56 (MQ=255)

                                cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCa                                                   >  1:1106369/1‑56 (MQ=255)

                                cTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACATTCTTTCa                                                   >  1:1520446/1‑56 (MQ=255)

                                cTGGTCGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCa                                                   >  1:2612240/1‑56 (MQ=255)

                                  ggTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                               >  1:3456690/1‑42 (MQ=255)

                                  ggTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                               >  1:2645625/1‑42 (MQ=255)

                                  ggTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                               >  1:2199071/1‑42 (MQ=255)

                                   gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                               >  1:2089969/1‑41 (MQ=255)

                                   gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                               >  1:2072748/1‑41 (MQ=255)

                                   gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                               >  1:3710495/1‑41 (MQ=255)

                                   gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                               >  1:3736611/1‑41 (MQ=255)

                                   gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGa                                                               >  1:1738719/1‑41 (MQ=255)

                                   gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTa                                 >  1:3280688/1‑71 (MQ=255)

                                   gTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATAACTTCTTTCAGGATCTTg                                           >  1:4029378/1‑61 (MQ=255)

                                      gAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:3045234/70‑1 (MQ=255)

                                      gAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:3063040/70‑1 (MQ=255)

                                      gAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:2848929/70‑1 (MQ=255)

                                      gAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:3555084/70‑1 (MQ=255)

                                      gAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:4326913/70‑1 (MQ=255)

                                         tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:1796853/67‑1 (MQ=255)

                                         tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:1615638/67‑1 (MQ=255)

                                         tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACg                               <  1:1727563/67‑1 (MQ=255)

                                         tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:4881225/1‑69 (MQ=255)

                                         tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:5027547/1‑69 (MQ=255)

                                         tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:693836/1‑69 (MQ=255)

                                         tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:3720977/1‑69 (MQ=255)

                                         tttGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:627520/1‑69 (MQ=255)

                                           tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTAc                                >  1:3862310/1‑64 (MQ=255)

                                           tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:1075451/1‑67 (MQ=255)

                                           tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:2695796/1‑67 (MQ=255)

                                           tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:1150991/1‑67 (MQ=255)

                                           tGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGaa                             >  1:3665126/1‑67 (MQ=255)

                                            gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                              <  1:4529042/65‑1 (MQ=255)

                                            gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                              <  1:1320598/65‑1 (MQ=255)

                                            gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                              <  1:4012794/65‑1 (MQ=255)

                                            gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                              <  1:2690427/65‑1 (MQ=255)

                                            gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                              <  1:4309621/65‑1 (MQ=255)

                                            gATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGa                              <  1:492896/65‑1 (MQ=255)

                                              tCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcg                      <  1:840978/71‑1 (MQ=255)

                                              tCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcg                      <  1:3831546/71‑1 (MQ=255)

                                              tCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcg                      <  1:353336/71‑1 (MQ=255)

                                               cAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                         >  1:2928097/1‑51 (MQ=255)

                                               cAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                         >  1:4472023/1‑51 (MQ=255)

                                               cAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                         >  1:4845378/1‑51 (MQ=255)

                                               cAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                         >  1:3877099/1‑51 (MQ=255)

                                               cAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGgt                                         >  1:3745068/1‑51 (MQ=255)

                                               cAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                     >  1:2014484/1‑71 (MQ=255)

                                                 ggATCGAGTTGGCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAGgtcgtc                    <  1:2755873/70‑1 (MQ=255)

                                                  gATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAAAAGGTCGTCac                  <  1:4469081/71‑1 (MQ=255)

                                                    tGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATc                                              >  1:1876612/1‑41 (MQ=255)

                                                    tGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCacca                <  1:4911037/71‑1 (MQ=255)

                                                     ggAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtc                       <  1:4488300/63‑1 (MQ=255)

                                                     ggAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtc                       <  1:1833948/63‑1 (MQ=255)

                                                     ggAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtc                       <  1:3374327/63‑1 (MQ=255)

                                                       aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTg                                           >  1:1959907/1‑41 (MQ=255)

                                                       aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                     >  1:1147867/1‑63 (MQ=255)

                                                       aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                     >  1:1185690/1‑63 (MQ=255)

                                                       aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcgt                     >  1:5025373/1‑63 (MQ=255)

                                                       aGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGgtcg                      >  1:4222619/1‑62 (MQ=255)

                                                        gTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCaccaac              >  1:1411472/1‑69 (MQ=255)

                                                         ttAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAg           <  1:1934896/71‑1 (MQ=255)

                                                           ggCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAgg                         >  1:3174283/2‑55 (MQ=255)

                                                           ggCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAgg                         >  1:5188816/2‑55 (MQ=255)

                                                           ggCGATGCCCTTCTGGATGCCTTCCTTCAGGATCTTGGTGTTGGTGACGAACAgg                         >  1:18579/2‑55 (MQ=255)

                                                            gCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGGCACCAACCAg           <  1:1321393/68‑1 (MQ=255)

                                                                  ccTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGAtttt  <  1:2857281/71‑1 (MQ=255)

                                                                  ccTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGAtttt  <  1:1329090/71‑1 (MQ=255)

                                                                   cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                   >  1:650838/1‑53 (MQ=255)

                                                                   cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                   >  1:3494194/1‑53 (MQ=255)

                                                                   cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                   >  1:473569/1‑53 (MQ=255)

                                                                   cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                   >  1:443854/1‑53 (MQ=255)

                                                                   cTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCa                   >  1:4107189/1‑53 (MQ=255)

ATTGCAGCCAGAGTTTCGGTCAGAGAACCGATCTGGTTGAATTTGATCAGGATGGAGTTAGCGATACCTTTTTCGATACCTTCTTTCAGGATCTTGGTGTTGGTTACGAACAGGTCGTCACCAACCAGCTGGATTTT  >  Exported/1876357‑1876493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`