| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,065,013 | A→G | 5.2% | K418K (AAA→AAG) | rpoD → | RNA polymerase, sigma 70 (sigma D) factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,065,013 | 0 | A | G | 5.2% | 345.2 / 6.6 | 115 | K418K (AAA→AAG) | rpoD | RNA polymerase, sigma 70 (sigma D) factor |
| Reads supporting (aligned to +/- strand): ref base A (69/40); new base G (4/2); total (73/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
Only 100 of 122 total aligned reads displayed.
TGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCG > Exported/2064950‑2065073 | tGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAAt < 1:3085226/71‑1 (MQ=255)tGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAAt < 1:2571706/71‑1 (MQ=255)tGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAAt < 1:1216528/71‑1 (MQ=255)tGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAAt < 1:2272023/71‑1 (MQ=255)tGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAAt < 1:2268039/71‑1 (MQ=255)tGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAAt < 1:1285275/71‑1 (MQ=255) gCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAc > 1:4756893/1‑71 (MQ=255) gCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAc > 1:3951664/1‑71 (MQ=255) gCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAc > 1:1623571/1‑71 (MQ=255) gCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAc > 1:1426723/1‑71 (MQ=255) gCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATAc > 1:1420719/1‑71 (MQ=255) ccTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGt > 1:425082/1‑70 (MQ=255) cTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCg < 1:924103/55‑1 (MQ=255) ttGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTa > 1:5009297/1‑70 (MQ=255) tGACCTGATTCAGGAAGGCAAAATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTAc > 1:1820771/1‑70 (MQ=255) aCCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAg < 1:2093895/71‑1 (MQ=255) ccTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAg < 1:2491365/70‑1 (MQ=255) ccTGATCCAGGAAGGCAATATCGGCCTGATGAAGGCGGTGGACAAGTTCGAATACCGTCGCGGCTATaaat < 1:1108804/71‑3 (MQ=12) tGATCCAGGAAGGCAATATCGGCCTGATGAAGGCTGTGGACAAGTTCGAATACCGTCGCGGCTATaaat < 1:2569484/69‑3 (MQ=9) gATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGt > 1:2818843/1‑58 (MQ=255) gATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACaa > 1:491083/1‑66 (MQ=255) gATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACaa > 1:2829745/1‑66 (MQ=255) ttCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGt < 1:1495372/59‑1 (MQ=255) ttCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGt < 1:4595197/59‑1 (MQ=255) ttCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTcc < 1:1625803/71‑1 (MQ=255) ttCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTcc < 1:948051/71‑1 (MQ=255) ttCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTcc < 1:4621225/71‑1 (MQ=255) tCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa > 1:393144/1‑40 (MQ=255) tCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa > 1:4147094/1‑40 (MQ=255) tCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa > 1:5027191/1‑40 (MQ=255) tCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa > 1:60001/1‑40 (MQ=255) tCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa > 1:1851902/1‑40 (MQ=255) tCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATaaa > 1:1587890/1‑40 (MQ=255) ccAGGAAGGCAATATCGGCCTGCTCAAGGCCGTGGATAAATTCGAATACCGTCGCGGTTATAAATtctcga > 1:2428536/2‑69 (MQ=255) ccAGGAAGGCAATATCGGCCTGCTCAAGGCCGTGGATAAATTCGAATACCGTCGCGGTTATAAATtctcga > 1:3173542/2‑69 (MQ=255) ccAGGAAGGCAATATCGGCCTGCTCAAGGCCGTGGATAAATTCGAATACCGTCGCGGTTATAAATtctcga > 1:3170143/2‑69 (MQ=255) acAGGAAGGCAATATCGGCCTGCTCAAGGCCGTGGATAAATTCGAATACCGTCGCGGt > 1:1878456/2‑58 (MQ=255) gAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACa < 1:2127057/58‑1 (MQ=255) aaGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACa < 1:3690460/57‑1 (MQ=255) aGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTa > 1:5043968/1‑69 (MQ=255) aGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTa > 1:2254085/1‑69 (MQ=255) aGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTa > 1:1291949/1‑69 (MQ=255) aGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTATCCACCTa > 1:1578098/1‑69 (MQ=255) aCATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAAcc > 1:991692/1‑71 (MQ=255) aCATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAAcc > 1:4898665/1‑71 (MQ=255) aCATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAAcc < 1:3107784/71‑1 (MQ=255) taTCGGCCTGATGAAGGCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAAtt > 1:4970356/2‑54 (MQ=255) taTCGGCCTGATGAAGGCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAAtt > 1:2656642/2‑54 (MQ=255) taTCGGCCTGATGAAGGCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAAtt > 1:75893/2‑54 (MQ=255) cATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCt < 1:483580/71‑1 (MQ=255) cATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCt < 1:4866391/71‑1 (MQ=255) aTCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACaa > 1:2294084/1‑50 (MQ=255) aTCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACaa > 1:2774363/1‑50 (MQ=255) aTCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACaa > 1:742241/1‑50 (MQ=255) aTCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACaa > 1:3728995/1‑50 (MQ=255) aTCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACa > 1:72336/1‑49 (MQ=255) aTCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACa > 1:4060350/1‑49 (MQ=255) aTCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTtctc > 1:3423525/1‑56 (MQ=255) aTCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTtctc > 1:3295095/1‑56 (MQ=255) aTCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTtctc > 1:1387133/1‑56 (MQ=255) aTCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGc > 1:813768/1‑65 (MQ=255) aTCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGc > 1:2513981/1‑65 (MQ=255) aTCGGTCTGATGAAAGCGGTTGATAAATTCGAATAACGCCGTGGTTACAAGTtctc > 1:5133367/1‑56 (MQ=255) tCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCtgg < 1:3119972/71‑1 (MQ=255) cGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACa < 1:126012/47‑1 (MQ=255) tCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGa < 1:2469570/71‑1 (MQ=255) cTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCt > 1:4496882/1‑55 (MQ=255) cTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGAt > 1:1189773/1‑71 (MQ=255) cTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTATCCACCTACGCAACCTGGTGGAt > 1:5067196/1‑71 (MQ=255) tgatgaAGGCGGTGGACAAGTTCGAATACCGTCGCGGCTATAAATTCTAGACCTATGCGACGTGGTGCAtt > 1:4832722/1‑70 (MQ=255) tgatgaAAGCGGTTGATAAATTCGAATACCGCCGTGGt < 1:3878127/38‑1 (MQ=255) tgatgaAAGCGGTTGATAAATTCGAATACCGCCGTGGt < 1:1532944/38‑1 (MQ=255) tgatgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAAc > 1:2689457/1‑61 (MQ=255) tgatgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAAc > 1:3162790/1‑61 (MQ=255) tgatgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATc > 1:3301009/1‑71 (MQ=255) atgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGtt > 1:4831013/1‑44 (MQ=255) atgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGt > 1:4011548/1‑43 (MQ=255) atgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATcc < 1:2333211/70‑1 (MQ=255) atgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATcc > 1:3176972/1‑70 (MQ=255) atgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATcc < 1:2991830/70‑1 (MQ=255) atgaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCg > 1:523219/1‑71 (MQ=255) gaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAg > 1:4738799/1‑40 (MQ=255) gaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAg > 1:2001162/1‑40 (MQ=255) gaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAg > 1:2834245/1‑40 (MQ=255) gaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAg > 1:4143497/1‑40 (MQ=255) gaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAg > 1:2190888/1‑40 (MQ=255) gaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAg > 1:204568/1‑40 (MQ=255) gaAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAg > 1:2045447/1‑40 (MQ=255) aaaGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGt > 1:1267602/1‑69 (MQ=255) aaGCGGTTGATAAATTCGAATACCGCCGTTGTTACAAGTTCTcc < 1:1189966/44‑1 (MQ=255) cGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCg < 1:1617499/71‑1 (MQ=255) ttGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCt < 1:4622198/42‑1 (MQ=255) ttGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCt < 1:868206/42‑1 (MQ=255) ttGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCt < 1:877670/42‑1 (MQ=255) ttGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGt < 1:2898550/62‑1 (MQ=255) aTAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCa > 1:616585/1‑61 (MQ=255) aTAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCa > 1:4811615/1‑61 (MQ=255) aTAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCa > 1:2426196/1‑61 (MQ=255) aTAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCa > 1:3553761/1‑61 (MQ=255) aTAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCa > 1:1089195/1‑61 (MQ=255) | TGGCTTGCAGTTCCTTGACCTGATTCAGGAAGGCAACATCGGTCTGATGAAAGCGGTTGATAAATTCGAATACCGCCGTGGTTACAAGTTCTCCACCTACGCAACCTGGTGGATCCGTCAGGCG > Exported/2064950‑2065073 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |