| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,155,567 | A→T | 7.3% | intergenic (+78/+108) | dacB → / ← obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,155,567 | 0 | A | T | 7.3% | 198.0 / 6.8 | 68 | intergenic (+78/+108) | dacB/obgE | D‑alanyl‑D‑alanine carboxypeptidase/GTPase involved in cell partioning and DNA repair |
| Reads supporting (aligned to +/- strand): ref base A (33/30); new base T (3/2); total (36/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01 | |||||||||||
CGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGA > Exported/2155504‑2155631 | cGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCt > 1:735555/1‑71 (MQ=255)cGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCt > 1:4380898/1‑71 (MQ=255) ggCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCgg > 1:1751575/1‑70 (MQ=255) ggCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGc > 1:841965/1‑71 (MQ=255) ggCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAAAGCCTTATCCGGc > 1:222517/1‑71 (MQ=255) ggCCGCGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa > 1:4171027/1‑57 (MQ=255) tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCt < 1:4326983/62‑1 (MQ=11) tacAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCt < 1:4278650/62‑1 (MQ=11) tCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGcc > 1:1863045/1‑52 (MQ=255) cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAc > 1:2267739/1‑64 (MQ=255) cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAc > 1:4166163/1‑64 (MQ=255) cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAc > 1:3280805/1‑64 (MQ=255) aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaaa > 1:3370355/1‑46 (MQ=255) aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaaa > 1:2148579/1‑46 (MQ=255) aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:296044/44‑1 (MQ=255) aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:893721/44‑1 (MQ=255) aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:656312/44‑1 (MQ=255) aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:2551119/44‑1 (MQ=255) aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:763949/44‑1 (MQ=255) aGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:982521/44‑1 (MQ=255) gATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:2477515/43‑1 (MQ=255) gATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:3902879/43‑1 (MQ=255) attattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:4047696/42‑1 (MQ=255) attattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:2408081/42‑1 (MQ=255) attattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:1468123/42‑1 (MQ=255) attattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:777712/42‑1 (MQ=255) attattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:1085459/42‑1 (MQ=255) attattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTa < 1:4628730/42‑1 (MQ=255) ttattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAt > 1:4585261/1‑65 (MQ=255) ttattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGc > 1:4591056/1‑70 (MQ=255) tattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaaa > 1:3627590/1‑42 (MQ=255) tattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCa > 1:3840130/1‑70 (MQ=255) tattGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCa > 1:2172985/1‑70 (MQ=255) attGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCaaa > 1:3062189/1‑71 (MQ=255) attGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCaaa > 1:2696007/1‑71 (MQ=255) ttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa > 1:3825916/1‑39 (MQ=255) ttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa > 1:4558254/1‑39 (MQ=255) ttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa > 1:5028588/1‑39 (MQ=255) ttGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTaa > 1:1994998/1‑39 (MQ=255) aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc > 1:4966365/1‑70 (MQ=39) aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc > 1:890982/1‑70 (MQ=39) aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTc > 1:1896606/1‑70 (MQ=39) aaGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACa < 1:3366625/71‑1 (MQ=38) aaGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACa < 1:2709058/71‑1 (MQ=38) aaGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACa < 1:4567447/71‑1 (MQ=38) gTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACata > 1:1896784/1‑71 (MQ=39) gcgcTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACatat > 1:3784885/1‑70 (MQ=40) gcgcTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACata > 1:2425712/1‑69 (MQ=40) gcgcTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATAtt > 1:1823167/1‑71 (MQ=39) tATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTctct > 1:94848/1‑71 (MQ=255) aTGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTg < 1:4788437/65‑1 (MQ=255) aTGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTg < 1:2546913/65‑1 (MQ=255) aTGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGcc < 1:3762875/71‑1 (MQ=255) aTGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGcc < 1:1618627/71‑1 (MQ=255) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCaaa > 1:3525351/1‑41 (MQ=38) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCaaa > 1:5062843/1‑41 (MQ=38) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCaaa > 1:2067385/1‑41 (MQ=38) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTGAACatat > 1:492877/1‑51 (MQ=255) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACatat > 1:3821918/1‑51 (MQ=255) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACatat > 1:4103143/1‑51 (MQ=255) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACatat > 1:2615585/1‑51 (MQ=255) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACatat > 1:1935699/1‑51 (MQ=255) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACatat > 1:1166009/1‑51 (MQ=255) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTg > 1:643270/1‑64 (MQ=255) tGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTa > 1:2311806/1‑66 (MQ=255) cggcgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGa < 1:2891642/71‑1 (MQ=255) ggcgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGa > 1:1019036/1‑70 (MQ=255) ggcgTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGa > 1:1006379/1‑70 (MQ=255) gTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGa < 1:3431574/67‑1 (MQ=255) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAAt < 1:1059413/35‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAAt < 1:3624694/35‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAAt < 1:4985056/35‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAAt < 1:1339078/35‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAAt < 1:1848199/35‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAAt < 1:1894353/35‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAAt < 1:738143/35‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa < 1:682341/39‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa < 1:855318/39‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa < 1:2035714/39‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa < 1:2463651/39‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa < 1:725111/39‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa < 1:4770464/39‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa < 1:2576052/39‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa < 1:2578548/39‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa < 1:582710/39‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa < 1:5156962/39‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa < 1:2872301/39‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa < 1:4539700/39‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCaa < 1:4664679/39‑1 (MQ=40) tAAACGCCTTATCCGGCCTACAAAATCGCGCAAATTCaa < 1:2118728/39‑1 (MQ=255) | CGGCACATGGCCGGGGCTTCAGATTATTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAATCGTGCAAATTCAACATATTGCAATTCTCTTGTAGGCCTGA > Exported/2155504‑2155631 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |