| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 109,695 | C→T | 14.7% | H647Y (CAC→TAC) | aceE → | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 109,695 | 0 | C | T | 14.7% | 150.3 / 16.4 | 62 | H647Y (CAC→TAC) | aceE | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base C (19/33); new base T (7/2); total (26/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.95e-01 | |||||||||||
CTGATCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCAT > Exported/109626‑109765 | cTGATCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCca > 1:4869140/1‑71 (MQ=255)cTGATCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCca > 1:4986175/1‑71 (MQ=255)cTGATCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCca > 1:3748247/1‑71 (MQ=255) gATCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCcaca > 1:4488963/1‑71 (MQ=255) gATCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCcaca > 1:3778329/1‑71 (MQ=255) tCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGGCACAtt < 1:1969520/71‑1 (MQ=255) tCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACAtt < 1:1128471/71‑1 (MQ=255) tCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACAtt < 1:3435891/71‑1 (MQ=255) tCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACAtt < 1:1721085/71‑1 (MQ=255) ggcggTACTTCCGGTCGTACCACCCTGAACGTCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTc < 1:3333204/70‑1 (MQ=255) ggcggTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTc < 1:106912/70‑1 (MQ=255) ggcggTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTc < 1:3839412/70‑1 (MQ=255) ggcggTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCa < 1:4323920/71‑1 (MQ=255) ggcggTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCAACATTCa < 1:1727404/71‑1 (MQ=255) cTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCt < 1:4076172/70‑1 (MQ=255) cTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCt > 1:2036212/1‑70 (MQ=255) cTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCt > 1:3036260/1‑70 (MQ=255) cTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCt > 1:2544627/1‑70 (MQ=255) ccGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGAc < 1:2328172/70‑1 (MQ=255) cGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATccc > 1:2794630/1‑71 (MQ=255) ccaccCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCa > 1:945725/1‑52 (MQ=255) ccaccCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCa > 1:698482/1‑52 (MQ=255) accCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTg < 1:2165794/71‑1 (MQ=255) cccTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTg < 1:4539368/70‑1 (MQ=255) cccTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTg < 1:2759283/70‑1 (MQ=255) gAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGAc < 1:2641005/54‑1 (MQ=255) gAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGAc < 1:2593176/54‑1 (MQ=255) gAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGAc < 1:250673/54‑1 (MQ=255) gAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGAc < 1:377055/54‑1 (MQ=255) ggCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACAtt < 1:4217739/39‑1 (MQ=255) ggCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACAtt < 1:687377/39‑1 (MQ=255) ggCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTa < 1:4637397/71‑1 (MQ=255) ggCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTa < 1:4667080/71‑1 (MQ=255) gCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTAc > 1:2618749/1‑71 (MQ=255) cGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACg < 1:4663100/71‑1 (MQ=255) aGGTCTGCAGCACGAAGATGGTCACAGCTACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGAcc > 1:1289223/1‑71 (MQ=255) aGGTCTGCAGCACGAAGATGGTCACAGCTACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGAcc > 1:3597082/1‑71 (MQ=255) aGGTCTGCAGCACGAAGATGGTCACAGCTACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGAcc > 1:3536865/1‑71 (MQ=255) aGGTCTGCAGCACGAAGATGGTCACAGCTACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGAcc > 1:2033612/1‑71 (MQ=255) aGGTCTGCAGCACGAAGATGGTCACAGCTACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGAcc > 1:168373/1‑71 (MQ=255) aGGTCTGCAGCACGAAGATGGTCACAGCTACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGAcc > 1:244504/1‑71 (MQ=255) aGGTCTGCAGCACGAAGATGGTCACAGCTACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGAcc > 1:2468025/1‑71 (MQ=255) aGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGAcc > 1:5083203/1‑71 (MQ=255) aGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGAcc > 1:3103786/1‑71 (MQ=255) tCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCgg < 1:1251081/71‑1 (MQ=255) tCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCgg < 1:3448589/71‑1 (MQ=255) tCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCg > 1:2030991/1‑70 (MQ=255) tGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATctct < 1:2665599/59‑1 (MQ=255) tGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATctct < 1:744967/59‑1 (MQ=255) tGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATctct < 1:2889208/59‑1 (MQ=255) gcagcaCGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGgct > 1:3360042/1‑70 (MQ=255) gcagcaCGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGgct > 1:4530336/1‑70 (MQ=255) gcagcaCGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGgct > 1:970608/1‑70 (MQ=255) cagcaCGAAGATGGTCACAGCTACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGgctta < 1:1209154/71‑1 (MQ=255) cagcaCGAAGATGGTCACAGCTACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGgctta < 1:1657897/71‑1 (MQ=255) cagcaCGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACg < 1:4939755/61‑1 (MQ=255) cagcaCGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACg < 1:1101214/61‑1 (MQ=255) cagcaCGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACg < 1:375717/61‑1 (MQ=255) cagcaCGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACg < 1:2789815/61‑1 (MQ=255) cGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGgcttacgct > 1:4720876/1‑70 (MQ=255) cGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGgcttacgct > 1:772327/1‑70 (MQ=255) aaGATGGTCACAGACACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTa < 1:4731453/52‑1 (MQ=255) acaGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCt > 1:4494903/1‑71 (MQ=255) acaGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCt > 1:4220570/1‑71 (MQ=255) acaGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCt > 1:3644930/1‑71 (MQ=255) acaGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCt > 1:2295975/1‑71 (MQ=255) gCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGtc < 1:99324/71‑1 (MQ=255) ccACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGtca > 1:1722807/1‑71 (MQ=255) cacaTTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGtcat < 1:1349106/71‑1 (MQ=255) | CTGATCGGCGGTACTTCCGGTCGTACCACCCTGAACGGCGAAGGTCTGCAGCACGAAGATGGTCACAGCCACATTCAGTCGCTGACTATCCCGAACTGTATCTCTTACGACCCGGCTTACGCTTACGAAGTTGCTGTCAT > Exported/109626‑109765 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |