Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,396,396 C→G 11.9% V264V (GTG→GTC corA ← magnesium/nickel/cobalt transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,396,3960CG11.9% 179.5 / 13.9 67V264V (GTG→GTCcorAmagnesium/nickel/cobalt transporter
Reads supporting (aligned to +/- strand):  ref base C (37/22);  new base G (3/5);  total (40/27)
Fisher's exact test for biased strand distribution p-value = 2.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

TTCAGTTCTGGCATAAACTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGC  >  Exported/2396328‑2396465
                                                                    |                                                                     
ttCAGTTCTGGCATAAACTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCAc                                                                     >  1:2626403/1‑71 (MQ=255)
ttCAGTTCTGGCATAAACTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCAc                                                                     >  1:5075900/1‑71 (MQ=255)
ttCAGTTCTGGCATAAACTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCAc                                                                     >  1:4681309/1‑71 (MQ=255)
ttCAGTTCTGGCATAAACTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCAc                                                                     >  1:1489538/1‑71 (MQ=255)
          gCATAAACTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATacca                                                             <  1:4314796/69‑1 (MQ=255)
               aaCTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCgag                                                        >  1:3349645/1‑69 (MQ=255)
               aaCTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCgag                                                        >  1:4509401/1‑69 (MQ=255)
               aaCTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCgag                                                        >  1:4151875/1‑69 (MQ=255)
                  tCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGAt                                                                 <  1:4545789/57‑1 (MQ=255)
                      aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATag                                                               >  1:1389904/1‑54 (MQ=255)
                      aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATac                                                               >  1:4255323/1‑55 (MQ=255)
                      aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATac                                                               >  1:2692619/1‑55 (MQ=255)
                      aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATac                                                               >  1:1920749/1‑55 (MQ=255)
                      aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATac                                                               >  1:175046/1‑55 (MQ=255)
                      aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATac                                                               >  1:163453/1‑55 (MQ=255)
                      aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATac                                                               >  1:4434747/1‑55 (MQ=255)
                      aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATac                                                               >  1:477543/1‑55 (MQ=255)
                      aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt                                                >  1:3837953/1‑70 (MQ=255)
                      aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt                                                >  1:2085929/1‑70 (MQ=255)
                      aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt                                                >  1:3351686/1‑70 (MQ=255)
                      aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt                                                >  1:3221235/1‑70 (MQ=255)
                      aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt                                                >  1:2932107/1‑70 (MQ=255)
                      aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt                                                >  1:5139216/1‑70 (MQ=255)
                      aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACCGATac                                                               >  1:4440803/1‑55 (MQ=255)
                        ttCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTtgat                                             <  1:401522/71‑1 (MQ=255)
                        ttCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTtgat                                             <  1:1142886/71‑1 (MQ=255)
                            tGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACTGAGAAGATTTTgatgat                                          >  1:3294223/1‑70 (MQ=255)
                            tGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTgatgat                                          >  1:98226/1‑70 (MQ=255)
                            tGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGatgatg                                         >  1:2458664/1‑71 (MQ=255)
                                   gCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCt                                  >  1:2531082/1‑71 (MQ=255)
                                   gCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCt                                  >  1:3331190/1‑71 (MQ=255)
                                       gAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTc                              <  1:2526692/71‑1 (MQ=255)
                                       gAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGAAGATGCGGTTCTGCTc                              <  1:4773092/71‑1 (MQ=255)
                                        aaGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTtgat                                             <  1:1574528/55‑1 (MQ=255)
                                         aGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGa                            <  1:2167132/71‑1 (MQ=255)
                                         aGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGCTCTGCTCGa                            <  1:3130823/71‑1 (MQ=255)
                                          gCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGAt                           <  1:3354515/71‑1 (MQ=255)
                                                   gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa                  <  1:1813713/71‑1 (MQ=255)
                                                   gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa                  <  1:2447158/71‑1 (MQ=255)
                                                   gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa                  <  1:5057904/71‑1 (MQ=255)
                                                   gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa                  <  1:5072763/71‑1 (MQ=255)
                                                   gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa                  <  1:549962/71‑1 (MQ=255)
                                                    tCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATaaaa                 >  1:258502/1‑71 (MQ=255)
                                                      ggcggcAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaagc                >  1:4217374/1‑68 (MQ=255)
                                                      ggcggcAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATGAAGcc               >  1:1692191/1‑71 (MQ=255)
                                                      ggcggcAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATGAAGcc               >  1:4184570/1‑71 (MQ=255)
                                                          gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt                     <  1:1228375/61‑1 (MQ=255)
                                                          gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt                     <  1:1047516/61‑1 (MQ=255)
                                                          gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt                     <  1:3384193/61‑1 (MQ=255)
                                                          gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt                     <  1:4141456/61‑1 (MQ=255)
                                                          gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt                     <  1:3557998/61‑1 (MQ=255)
                                                          gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt                     <  1:1777772/61‑1 (MQ=255)
                                                          gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAtt           <  1:1087850/71‑1 (MQ=255)
                                                          gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAtt           <  1:2477986/71‑1 (MQ=255)
                                                           cAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt            >  1:812618/1‑69 (MQ=255)
                                                           cAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt            >  1:3800745/1‑69 (MQ=255)
                                                            aGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATaa                   >  1:251984/1‑61 (MQ=255)
                                                              gAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt            >  1:1695809/1‑66 (MQ=255)
                                                              gAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt            >  1:2162886/1‑66 (MQ=255)
                                                              gAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt            >  1:1675692/1‑66 (MQ=255)
                                                                ataccacggataccacggAGAAGATTTTGATGCTGCGGTTCTGCTCGATGTTGATAAAAccc              >  1:2891766/1‑62 (MQ=255)
                                                                aTACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATa                    >  1:4469958/1‑56 (MQ=255)
                                                                aTACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAAccc              >  1:4330856/1‑62 (MQ=255)
                                                                 tACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCt    >  1:1174252/1‑71 (MQ=255)
                                                                  aCCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGc                                <  1:3525462/42‑1 (MQ=255)
                                                                  aCCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGc                                <  1:1383812/42‑1 (MQ=255)
                                                                  aCCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGc                                <  1:699964/42‑1 (MQ=255)
                                                                  aCCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGc                                <  1:1085906/42‑1 (MQ=255)
                                                                   ccACGGATACCACCGAGAAGATTTTTATGATGCTGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGc  <  1:2257654/71‑1 (MQ=255)
                                                                   ccACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCTATGTTGATAAAACCCATTGCCGCCTGc  <  1:4529187/71‑1 (MQ=255)
                                                                   ccACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGc  <  1:4311684/71‑1 (MQ=255)
                                                                   ccACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGc  <  1:824486/71‑1 (MQ=255)
                                                                    cACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATg                          <  1:2826970/46‑1 (MQ=255)
                                                                    cACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATg                          <  1:1131899/46‑1 (MQ=255)
                                                                    cACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATg                          <  1:1138150/46‑1 (MQ=255)
                                                                    |                                                                     
TTCAGTTCTGGCATAAACTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGC  >  Exported/2396328‑2396465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: