Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,396,399 G→C 12.4% S263S (TCC→TCG corA ← magnesium/nickel/cobalt transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,396,3990GC12.4% 198.5 / 15.6 72S263S (TCC→TCGcorAmagnesium/nickel/cobalt transporter
Reads supporting (aligned to +/- strand):  ref base G (33/30);  new base C (4/5);  total (37/35)
Fisher's exact test for biased strand distribution p-value = 7.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATAAACTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCAT  >  Exported/2396338‑2396467
                                                             |                                                                    
gCATAAACTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATacca                                                               <  1:4314796/69‑1 (MQ=255)
     aaCTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCgag                                                          >  1:3349645/1‑69 (MQ=255)
     aaCTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCgag                                                          >  1:4509401/1‑69 (MQ=255)
     aaCTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCgag                                                          >  1:4151875/1‑69 (MQ=255)
        tCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGAt                                                                   <  1:4545789/57‑1 (MQ=255)
            aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATag                                                                 >  1:1389904/1‑54 (MQ=255)
            aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATac                                                                 >  1:2692619/1‑55 (MQ=255)
            aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATac                                                                 >  1:1920749/1‑55 (MQ=255)
            aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATac                                                                 >  1:175046/1‑55 (MQ=255)
            aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATac                                                                 >  1:163453/1‑55 (MQ=255)
            aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATac                                                                 >  1:4255323/1‑55 (MQ=255)
            aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATac                                                                 >  1:4434747/1‑55 (MQ=255)
            aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATac                                                                 >  1:477543/1‑55 (MQ=255)
            aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt                                                  >  1:3837953/1‑70 (MQ=255)
            aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt                                                  >  1:3351686/1‑70 (MQ=255)
            aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt                                                  >  1:5139216/1‑70 (MQ=255)
            aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt                                                  >  1:2085929/1‑70 (MQ=255)
            aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt                                                  >  1:3221235/1‑70 (MQ=255)
            aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGAtttt                                                  >  1:2932107/1‑70 (MQ=255)
            aGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACCGATac                                                                 >  1:4440803/1‑55 (MQ=255)
              ttCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTtgat                                               <  1:401522/71‑1 (MQ=255)
              ttCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTtgat                                               <  1:1142886/71‑1 (MQ=255)
                  tGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACTGAGAAGATTTTgatgat                                            >  1:3294223/1‑70 (MQ=255)
                  tGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTgatgat                                            >  1:98226/1‑70 (MQ=255)
                  tGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGatgatg                                           >  1:2458664/1‑71 (MQ=255)
                         gCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCt                                    >  1:3331190/1‑71 (MQ=255)
                         gCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCt                                    >  1:2531082/1‑71 (MQ=255)
                             gAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTc                                <  1:2526692/71‑1 (MQ=255)
                             gAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGAAGATGCGGTTCTGCTc                                <  1:4773092/71‑1 (MQ=255)
                              aaGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTtgat                                               <  1:1574528/55‑1 (MQ=255)
                               aGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGa                              <  1:2167132/71‑1 (MQ=255)
                               aGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGCTCTGCTCGa                              <  1:3130823/71‑1 (MQ=255)
                                gCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGAt                             <  1:3354515/71‑1 (MQ=255)
                                         gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa                    <  1:5057904/71‑1 (MQ=255)
                                         gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa                    <  1:5072763/71‑1 (MQ=255)
                                         gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa                    <  1:2447158/71‑1 (MQ=255)
                                         gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa                    <  1:549962/71‑1 (MQ=255)
                                         gTCGGCGGCAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaa                    <  1:1813713/71‑1 (MQ=255)
                                          tCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATaaaa                   >  1:258502/1‑71 (MQ=255)
                                            ggcggcAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATgaagc                  >  1:4217374/1‑68 (MQ=255)
                                            ggcggcAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATGAAGcc                 >  1:1692191/1‑71 (MQ=255)
                                            ggcggcAGGAAGGCGACCGAGGCCACCGAGAAGATCTTGATGATCTTGTTCTGGTTGATGTTGATGAAGcc                 >  1:4184570/1‑71 (MQ=255)
                                                gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt                       <  1:3384193/61‑1 (MQ=255)
                                                gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt                       <  1:1047516/61‑1 (MQ=255)
                                                gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt                       <  1:1228375/61‑1 (MQ=255)
                                                gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt                       <  1:4141456/61‑1 (MQ=255)
                                                gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt                       <  1:3557998/61‑1 (MQ=255)
                                                gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGAt                       <  1:1777772/61‑1 (MQ=255)
                                                gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAtt             <  1:2477986/71‑1 (MQ=255)
                                                gcAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAtt             <  1:1087850/71‑1 (MQ=255)
                                                 cAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt              >  1:3800745/1‑69 (MQ=255)
                                                 cAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt              >  1:812618/1‑69 (MQ=255)
                                                  aGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATaa                     >  1:251984/1‑61 (MQ=255)
                                                    gAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt              >  1:1695809/1‑66 (MQ=255)
                                                    gAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt              >  1:2162886/1‑66 (MQ=255)
                                                    gAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCAt              >  1:1675692/1‑66 (MQ=255)
                                                      ataccacggataccacggAGAAGATTTTGATGCTGCGGTTCTGCTCGATGTTGATAAAAccc                >  1:2891766/1‑62 (MQ=255)
                                                      aTACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATa                      >  1:4469958/1‑56 (MQ=255)
                                                      aTACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAAccc                >  1:4330856/1‑62 (MQ=255)
                                                       tACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCt      >  1:1174252/1‑71 (MQ=255)
                                                        aCCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGc                                  <  1:1383812/42‑1 (MQ=255)
                                                        aCCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGc                                  <  1:699964/42‑1 (MQ=255)
                                                        aCCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGc                                  <  1:3525462/42‑1 (MQ=255)
                                                        aCCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGc                                  <  1:1085906/42‑1 (MQ=255)
                                                         ccACGGATACCACCGAGAAGATTTTTATGATGCTGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGc    <  1:2257654/71‑1 (MQ=255)
                                                         ccACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCTATGTTGATAAAACCCATTGCCGCCTGc    <  1:4529187/71‑1 (MQ=255)
                                                         ccACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGc    <  1:4311684/71‑1 (MQ=255)
                                                         ccACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGc    <  1:824486/71‑1 (MQ=255)
                                                          cACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATg                            <  1:2826970/46‑1 (MQ=255)
                                                          cACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATg                            <  1:1131899/46‑1 (MQ=255)
                                                          cACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATg                            <  1:1138150/46‑1 (MQ=255)
                                                           aCGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTc                                <  1:2991302/41‑1 (MQ=255)
                                                           aCGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCAt  >  1:4366902/1‑71 (MQ=255)
                                                             ggATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCAt  >  1:3429463/1‑69 (MQ=255)
                                                             ggATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCAt  >  1:2249070/1‑69 (MQ=255)
                                                             |                                                                    
GCATAAACTCAAAGTTCATGCCATAGCTGGAAGCAACGAGCGTCGGCGGCAGGAATACCACGGATACCACCGAGAAGATTTTGATGATGCGGTTCTGCTCGATGTTGATAAAACCCATTGCCGCCTGCAT  >  Exported/2396338‑2396467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: