| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,526,132 | T→C | 6.5% | A142A (GCT→GCC) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,526,132 | 0 | T | C | 6.5% | 372.4 / 11.5 | 123 | A142A (GCT→GCC) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (57/58); new base C (2/6); total (59/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 123 total aligned reads displayed.
GTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTT > Exported/2526064‑2526199 | gTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCATGCTaa > 1:5066079/1‑71 (MQ=255) tAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAAt < 1:4350515/71‑1 (MQ=255) tAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAAt < 1:4393075/71‑1 (MQ=255) tAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAAt < 1:701954/71‑1 (MQ=255) tAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAAt < 1:3583730/71‑1 (MQ=255) tAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAAt < 1:2184225/71‑1 (MQ=255) aaTGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAAt < 1:2947112/70‑1 (MQ=255) cTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa > 1:4298422/1‑65 (MQ=255) cTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa > 1:3474949/1‑65 (MQ=255) cTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa > 1:1653833/1‑65 (MQ=255) cTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcgc < 1:4995444/71‑1 (MQ=255) ggTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcgc < 1:3986394/69‑1 (MQ=255) ggTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcgc < 1:2401253/69‑1 (MQ=255) ggTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcgc < 1:5044256/69‑1 (MQ=255) ggTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGgcgc < 1:169013/69‑1 (MQ=255) gTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGa < 1:4070227/70‑1 (MQ=255) gatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGa > 1:2087595/1‑64 (MQ=255) gatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGa > 1:2688692/1‑64 (MQ=255) gatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGa > 1:4286637/1‑64 (MQ=255) gatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGa > 1:2731053/1‑64 (MQ=255) gatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGa > 1:1368359/1‑64 (MQ=255) gatgTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGa > 1:458041/1‑64 (MQ=255) gATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa > 1:5113172/1‑51 (MQ=255) gATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa > 1:4667008/1‑51 (MQ=255) gATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa > 1:2875129/1‑51 (MQ=255) gATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCg > 1:3789346/1‑71 (MQ=255) gATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCg > 1:2781869/1‑71 (MQ=255) gATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCg > 1:1675212/1‑71 (MQ=255) gATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCg > 1:3610880/1‑71 (MQ=255) aTCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCg > 1:9118/1‑70 (MQ=255) aCCGCCGGAACGGCGATTCGCGAGTCGATGGAAATTATTCAGGCCAATGGCGCGACGCTTGCAGGCGTGtt < 1:1948679/71‑1 (MQ=255) aCCGCCGGAACGGCGATTCGCGAGTCGATGGAAATTATTCAGGCCAATGGCGCGACGCTTGCAGGCGTGtt < 1:3826910/71‑1 (MQ=255) aCCGCCGGAACGGCGATTCGCGAGTCGATGGAAATTATTCAGGCCAATGGCGCGACGCTTGCAGGCGTGtt < 1:4545109/71‑1 (MQ=255) cgccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGa < 1:335526/71‑1 (MQ=255) cgccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGa < 1:4552598/71‑1 (MQ=255) gccgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAt > 1:1386170/1‑71 (MQ=255) cgGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAttt < 1:2995334/71‑1 (MQ=255) ggAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAttt < 1:1167571/70‑1 (MQ=255) ggAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTc > 1:1567972/1‑71 (MQ=255) gAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCg < 1:2540319/71‑1 (MQ=255) gAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCg < 1:509088/71‑1 (MQ=255) gAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCg < 1:3602513/71‑1 (MQ=255) aCGGCGATTCGCGAATCGATGGAGATTATTCAGGCTaa > 1:57135/1‑38 (MQ=255) aCGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGc < 1:3364586/70‑1 (MQ=255) aCGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCt > 1:3189930/1‑71 (MQ=255) aCGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCt > 1:2207348/1‑71 (MQ=255) cGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCtt > 1:2655230/1‑71 (MQ=255) cGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCtt > 1:1717276/1‑71 (MQ=255) cGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCt > 1:3802852/1‑70 (MQ=255) gCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTg > 1:1355276/1‑70 (MQ=255) gCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGa < 1:4708359/71‑1 (MQ=255) gCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGa < 1:2359869/71‑1 (MQ=255) gCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGa < 1:1503985/71‑1 (MQ=255) aTTCGCGAGTCGATGGAAATTATTCAGGCCAATGGCGCGACGCTTGCAGGCGTGtt < 1:931789/56‑1 (MQ=255) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:3203008/71‑1 (MQ=255) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:4430620/71‑1 (MQ=255) ttCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt < 1:1813623/71‑1 (MQ=255) tCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATc < 1:930754/68‑1 (MQ=255) tCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATc < 1:3668100/68‑1 (MQ=255) tCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATc < 1:3444885/68‑1 (MQ=255) tCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATc < 1:4612966/68‑1 (MQ=255) cgcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt > 1:3101991/1‑69 (MQ=255) cgcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt > 1:2176534/1‑69 (MQ=255) cgcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGt > 1:3989636/1‑69 (MQ=255) cgcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTc < 1:3382243/70‑1 (MQ=255) cgcgAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTc < 1:333901/70‑1 (MQ=255) agtCGATGGAGATTATTCAGGCCAATGGCGCGACGCTTGCTGGCGTGTTGATTTCGc > 1:4105961/3‑57 (MQ=255) aaTCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGaa < 1:1515140/71‑1 (MQ=255) aTCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCTTATTGATTTCGCTTGAt > 1:2449045/1‑61 (MQ=255) aTCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGAttt < 1:1466031/53‑1 (MQ=255) aTCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGAt > 1:4755970/1‑61 (MQ=255) aTCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGAt > 1:3819201/1‑61 (MQ=255) aTCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGAt > 1:3872310/1‑61 (MQ=255) aTCGATGGAGATTAATCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGAt > 1:418557/1‑61 (MQ=255) gagaTTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGgcg < 1:1439874/71‑1 (MQ=255) aaattattCAGGCCAATGGCGCGACGCTTGCAGGCGTGtt < 1:1927061/38‑1 (MQ=25) attattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCtt < 1:116802/48‑1 (MQ=255) attattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCtt < 1:3146338/48‑1 (MQ=255) attattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCtt < 1:2330446/48‑1 (MQ=255) attattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCtt < 1:4931546/48‑1 (MQ=255) attattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCtt < 1:267229/48‑1 (MQ=255) tattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc > 1:3737231/1‑70 (MQ=255) tattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc > 1:4687147/1‑70 (MQ=255) tattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc > 1:835618/1‑70 (MQ=255) tattCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCgcggc > 1:652822/1‑70 (MQ=255) ttCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCg < 1:4924159/49‑1 (MQ=255) ttCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCg < 1:332421/49‑1 (MQ=255) ttCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCg < 1:4071926/49‑1 (MQ=255) ttCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCg < 1:3393762/49‑1 (MQ=255) ttCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCg < 1:756836/49‑1 (MQ=255) ttCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCg < 1:602590/49‑1 (MQ=255) ttCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCga < 1:5112914/70‑1 (MQ=255) tCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAg > 1:775077/1‑52 (MQ=255) tCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAg > 1:1879797/1‑52 (MQ=255) tCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAg > 1:3924447/1‑52 (MQ=255) tCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAg > 1:5148637/1‑52 (MQ=255) tCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAg > 1:5069047/1‑52 (MQ=255) tCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAg > 1:4883271/1‑52 (MQ=255) tCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAg > 1:4701396/1‑52 (MQ=255) ggCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGAttt < 1:321315/71‑1 (MQ=255) | GTAATGCTGGTAGATGATGTGATCACCGCCGGAACGGCGATTCGCGAATCGATGGAGATTATTCAGGCTAATGGCGCGACGCTTGCTGGCGTATTGATTTCGCTTGATCGTCAGGAACGGGGGCGCGGCGAGATTT > Exported/2526064‑2526199 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |