| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,545,261 | C→T | 5.2% | R79H (CGT→CAT) ‡ | rfaC ← | ADP‑heptose:LPS heptosyl transferase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,545,261 | 0 | C | T | 5.2% | 426.7 / 7.2 | 133 | R79H (CGT→CAT) ‡ | rfaC | ADP‑heptose:LPS heptosyl transferase I |
| Reads supporting (aligned to +/- strand): ref base C (38/88); new base T (2/5); total (40/93) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
Only 100 of 139 total aligned reads displayed.
CAGCGCCGCGCTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCGACGT > Exported/2545191‑2545320 | cAGCGCCGCGCTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCAc < 1:3038880/71‑1 (MQ=255)cAGCGCCGCGCTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCAc > 1:2947761/1‑71 (MQ=255)cAGCGCCGCGCTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCAc > 1:5093466/1‑71 (MQ=255) gcgcCGCGCTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACg > 1:4525033/1‑70 (MQ=255) gcgcCGCGCTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGa < 1:102965/71‑1 (MQ=255) gcgcCGCGCTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGa < 1:2600203/71‑1 (MQ=255) gcgcCGCGCTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGa < 1:4365752/71‑1 (MQ=255) gcgcCGCGCTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGa < 1:1774763/71‑1 (MQ=255) gcgcCGCGCTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGa < 1:1468892/71‑1 (MQ=255) ccgcGCTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAAc < 1:4344104/71‑1 (MQ=255) ccgcGCTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAAc < 1:3804144/71‑1 (MQ=255) ccgcGCTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAAc < 1:3979380/71‑1 (MQ=255) ccgcGCTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAAc < 1:1108369/71‑1 (MQ=255) ccgcGCTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAAc < 1:1621691/71‑1 (MQ=255) ccgcGCTTTTTACAAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAAc > 1:5088682/1‑71 (MQ=255) cTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:498238/71‑1 (MQ=255) cTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:3759455/71‑1 (MQ=255) cTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:2712986/71‑1 (MQ=255) cTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:1150426/71‑1 (MQ=255) cTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:2125700/71‑1 (MQ=255) cTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:2036999/71‑1 (MQ=255) tttttACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGTTTCGTGAAGCGCt < 1:4257384/68‑1 (MQ=255) tttttACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGTTTCGTGAAGCGCt < 1:1000565/68‑1 (MQ=255) ttttACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGa < 1:2791315/61‑1 (MQ=255) ttttACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGa < 1:4085135/61‑1 (MQ=255) ttttACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGc > 1:4708153/1‑71 (MQ=255) ttttACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGc > 1:4245418/1‑71 (MQ=255) ttttACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGc > 1:3616911/1‑71 (MQ=255) ttttACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGc > 1:3044812/1‑71 (MQ=255) tttACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGTTTCGTGAAGCGCtac > 1:3412417/1‑66 (MQ=37) tttACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGTTTCGTGAAGCGCtac > 1:1482223/1‑66 (MQ=37) tttACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCg > 1:4605049/1‑71 (MQ=255) tttACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCg > 1:3973205/1‑71 (MQ=255) ccAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTc < 1:4963426/70‑1 (MQ=255) ccAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTc < 1:521074/70‑1 (MQ=255) ccAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTc < 1:2505468/70‑1 (MQ=255) gCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCTCTTCACGa < 1:1094338/53‑1 (MQ=255) ccTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCtttt < 1:4574140/71‑1 (MQ=255) tGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:4081037/56‑1 (MQ=255) tGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:4114731/56‑1 (MQ=255) tGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:2176097/56‑1 (MQ=255) tGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:1905626/56‑1 (MQ=255) tGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:4996978/56‑1 (MQ=255) tGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCttt < 1:4771655/56‑1 (MQ=255) gAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATg > 1:3432281/1‑71 (MQ=255) gAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATg > 1:2790271/1‑71 (MQ=255) aGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTAt < 1:3329540/69‑1 (MQ=255) aGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATg < 1:1394545/70‑1 (MQ=255) aGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATg < 1:542549/70‑1 (MQ=255) gATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGcc > 1:2596579/1‑70 (MQ=255) tAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCgag < 1:3017815/71‑1 (MQ=255) tAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCgag < 1:2421958/71‑1 (MQ=255) tAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCgag < 1:4275101/71‑1 (MQ=255) aaCTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTAt > 1:3659309/1‑60 (MQ=255) aaCTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATg > 1:879637/1‑61 (MQ=255) aaCTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATg > 1:3548593/1‑61 (MQ=255) aaCTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTAGCGTTCCGCTTTTAt > 1:4556396/1‑60 (MQ=255) aCTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGTTTTTATGGGGGCCGAGaa < 1:3869313/71‑1 (MQ=255) aCTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGaa > 1:4544385/1‑71 (MQ=255) aTAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCtt > 1:922801/1‑47 (MQ=255) aTAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCtt > 1:1462039/1‑47 (MQ=255) aGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCttt > 1:3613310/1‑70 (MQ=255) aGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTa > 1:992030/1‑71 (MQ=255) aGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTa > 1:1843934/1‑71 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCttt < 1:3931862/44‑1 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCttt < 1:2736096/44‑1 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTa < 1:2368846/46‑1 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTa < 1:1630178/46‑1 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTa < 1:2546798/46‑1 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTa < 1:2884280/46‑1 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTa < 1:5152062/46‑1 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTa < 1:129987/69‑1 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTa < 1:4158796/69‑1 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTa < 1:1980312/69‑1 (MQ=255) ttCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTa < 1:2244955/69‑1 (MQ=255) ctctGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGc > 1:1103572/1‑70 (MQ=255) ctctGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGc > 1:5000827/1‑70 (MQ=255) gCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGc > 1:863079/1‑70 (MQ=255) cTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCg < 1:644275/70‑1 (MQ=255) cTTGTAGCGCTTCACGAAACGATTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCg < 1:4631338/70‑1 (MQ=255) gTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCGACg > 1:2249930/1‑70 (MQ=255) tAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCGACGt < 1:4423790/70‑1 (MQ=255) tAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCGACGt < 1:1980800/70‑1 (MQ=255) tAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCGACGt < 1:1387420/70‑1 (MQ=255) cgcTTCACGAAACGCTTTGCGTTCCTCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCTGCGACg < 1:4195220/66‑1 (MQ=255) cTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCtt < 1:4403758/51‑1 (MQ=255) cTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCtt < 1:4659195/51‑1 (MQ=255) cTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCtt < 1:2479647/51‑1 (MQ=255) cTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGCCCGAGAACCAGGCtt < 1:4432958/51‑1 (MQ=255) ttCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCtt < 1:865078/50‑1 (MQ=255) ttCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCGACg < 1:615741/63‑1 (MQ=255) tCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCgag > 1:1209617/1‑39 (MQ=255) tCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCgag > 1:1082024/1‑39 (MQ=255) tCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCgag > 1:2086242/1‑39 (MQ=255) tCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCgag > 1:2954640/1‑39 (MQ=255) tCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTa > 1:3884007/1‑51 (MQ=255) tCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTa > 1:2674269/1‑51 (MQ=255) tCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTa > 1:3322721/1‑51 (MQ=255) cGAAACGCTTTGCGTTCCGCTTTTATGGTGGCCGAGAACCAGGCtt < 1:1200934/46‑1 (MQ=255) cGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCtt < 1:1149178/46‑1 (MQ=255) | CAGCGCCGCGCTTTTTACCAGCCCCTGAGCGTCGATAACTGCGTCATAGTTCTCTGCTTGTAGCGCTTCACGAAACGCTTTGCGTTCCGCTTTTATGGGGGCCGAGAACCAGGCTTTACGCCAGCGACGT > Exported/2545191‑2545320 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |