Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 168,689 G→T 13.7% intergenic (‑358/‑10) map ← / → rpsB methionine aminopeptidase/30S ribosomal subunit protein S2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported168,6890GT13.7% 496.6 / 39.1 159intergenic (‑358/‑10)map/rpsBmethionine aminopeptidase/30S ribosomal subunit protein S2
Reads supporting (aligned to +/- strand):  ref base G (81/56);  new base T (14/8);  total (95/64)
Fisher's exact test for biased strand distribution p-value = 8.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01

Only 100 of 162 total aligned reads displayed.

CCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCC  >  Exported/168624‑168759
                                                                 |                                                                      
ccGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGttt                                                                    >  1:4881138/1‑70 (MQ=255)
ccGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGttt                                                                    >  1:4114337/1‑70 (MQ=255)
 cGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTa                                                                  <  1:4581833/71‑1 (MQ=255)
   gggTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAAt                                                                <  1:4132246/71‑1 (MQ=255)
     gTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCa                                                              <  1:3426222/71‑1 (MQ=255)
        cccTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTaa                                                                 >  1:2852746/1‑65 (MQ=255)
        cccTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTaa                                                                 >  1:1616854/1‑65 (MQ=255)
             tGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAAc                                                       <  1:1175690/70‑1 (MQ=255)
             tGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAAc                                                       <  1:2848966/70‑1 (MQ=255)
             tGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACt                                                      >  1:2466217/1‑71 (MQ=255)
               gggTCGGTAATATGGGATACGTGGAGGCATAACTCCAACTTTTATATAGAGGtttt                                                                   <  1:3083878/56‑1 (MQ=255)
               gggTCGGTAATATGGGATACGTGGAGGCATAACTCCAACTTTTATATAGAGGtttt                                                                   <  1:714316/56‑1 (MQ=255)
               gggTCGGTAATATGGGATACGTGGAGGCATAACTCCAACTTTTATATAGAGGtttt                                                                   <  1:2606077/56‑1 (MQ=255)
                   cGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTc                                                 >  1:3489493/1‑70 (MQ=255)
                    ggTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCa                                               >  1:985743/1‑71 (MQ=255)
                    ggTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCa                                               >  1:5181509/1‑71 (MQ=255)
                    ggTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCa                                               >  1:1460973/1‑71 (MQ=255)
                      tAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCaa                                                        <  1:2202933/60‑1 (MQ=255)
                           tGGGATAGGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGa                                        <  1:3691994/71‑1 (MQ=255)
                           tGGGATACGTGGAGGCATATCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGa                                        >  1:3280932/1‑71 (MQ=255)
                           tGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGa                                        >  1:4306886/1‑71 (MQ=255)
                           tGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGa                                        >  1:1667365/1‑71 (MQ=255)
                           tGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGa                                        >  1:1470981/1‑71 (MQ=255)
                           tGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGa                                        >  1:409097/1‑71 (MQ=255)
                             ggATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAAt                                                                <  1:2364909/45‑1 (MQ=255)
                             ggATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAAt                                                                <  1:1854306/45‑1 (MQ=255)
                             ggATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAAt                                                                <  1:4880860/45‑1 (MQ=255)
                             ggATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAAt                                                                <  1:378788/45‑1 (MQ=255)
                              gATACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAAt                                                                <  1:2382143/44‑1 (MQ=255)
                              gATACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAAt                                                                <  1:1585636/44‑1 (MQ=255)
                              gATACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAAt                                                                <  1:3624408/44‑1 (MQ=255)
                              gATACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAAt                                                                <  1:3793862/44‑1 (MQ=255)
                              gATACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAAt                                                                <  1:3857817/44‑1 (MQ=255)
                              gATACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAAt                                                                <  1:4728720/44‑1 (MQ=255)
                              gATACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAAt                                                                <  1:2039467/44‑1 (MQ=255)
                                tACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAATCa                                                              >  1:3493460/1‑44 (MQ=255)
                                tACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAATCa                                                              >  1:4130693/1‑44 (MQ=255)
                                tACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAATCa                                                              >  1:98085/1‑44 (MQ=255)
                                tACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAATCa                                                              >  1:4181778/1‑44 (MQ=255)
                                tACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAATCa                                                              >  1:3590051/1‑44 (MQ=255)
                                tACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAATCa                                                              >  1:162240/1‑44 (MQ=255)
                                tACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAATCa                                                              >  1:2494272/1‑44 (MQ=255)
                                tACGTGGAGGCATAACCCCAACTTTTATATAGATGTTTTAATCa                                                              >  1:1317931/1‑44 (MQ=255)
                                  cGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCa                                               >  1:4821762/1‑57 (MQ=255)
                                  cGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCa                                               >  1:5073019/1‑57 (MQ=255)
                                  cGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCa                                               >  1:5159843/1‑57 (MQ=255)
                                  cGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCt                                  >  1:4387552/1‑70 (MQ=255)
                                    tGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCTACt                                                      <  1:626943/48‑1 (MQ=255)
                                    tGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACt                                                      <  1:4160/48‑1 (MQ=255)
                                    tGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACt                                                      <  1:1858292/48‑1 (MQ=255)
                                    tGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACt                                                      <  1:4588854/48‑1 (MQ=255)
                                    tGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACt                                                      <  1:3824317/48‑1 (MQ=255)
                                    tGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACt                                                      <  1:3600942/48‑1 (MQ=255)
                                    tGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACt                                                      <  1:2764593/48‑1 (MQ=255)
                                     ggAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCa                                                              >  1:2884766/1‑39 (MQ=255)
                                     ggAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCa                                                              >  1:3906611/1‑39 (MQ=255)
                                     ggAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCa                                                              >  1:1187496/1‑39 (MQ=255)
                                     ggAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAg                              >  1:2594115/1‑71 (MQ=255)
                                     ggAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAg                              >  1:570274/1‑71 (MQ=255)
                                     ggAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAg                              >  1:854746/1‑71 (MQ=255)
                                     ggAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAg                              >  1:979996/1‑71 (MQ=255)
                                     ggAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAg                              >  1:4545925/1‑71 (MQ=255)
                                     ggAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAg                              >  1:2152434/1‑71 (MQ=255)
                                      gAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAgg                             <  1:356747/71‑1 (MQ=255)
                                        ggCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCt                           <  1:876118/71‑1 (MQ=255)
                                           aTAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGgt                        >  1:323203/1‑71 (MQ=255)
                                            tAACCCCAACTTTTATATAGAGGTTTTAATCATGGc                                                          <  1:4491042/36‑1 (MQ=255)
                                            tAACCCCAACTTTTATATAGAGGTTTTAATCATGGc                                                          <  1:1125778/36‑1 (MQ=255)
                                             aaCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATgcg                                           >  1:127083/1‑50 (MQ=255)
                                             aaCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATgcg                                           >  1:4804846/1‑50 (MQ=255)
                                              aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCa                                               >  1:2901590/1‑45 (MQ=255)
                                              aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAgg                             >  1:3000168/1‑63 (MQ=255)
                                              aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAgg                             >  1:2036783/1‑63 (MQ=255)
                                              aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAgg                             >  1:4111161/1‑63 (MQ=255)
                                              aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGgtgt                      >  1:2014862/1‑70 (MQ=255)
                                              aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGgtgt                      >  1:133109/1‑70 (MQ=255)
                                              aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGtt                     >  1:3303009/1‑71 (MQ=255)
                                              aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGtt                     >  1:217675/1‑71 (MQ=255)
                                              aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGtt                     <  1:838952/71‑1 (MQ=255)
                                              aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGtt                     >  1:2475077/1‑71 (MQ=255)
                                              aCCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGtt                     >  1:1034209/1‑71 (MQ=255)
                                               ccccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTc                    <  1:179770/71‑1 (MQ=255)
                                               ccccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTc                    <  1:3408116/71‑1 (MQ=255)
                                               ccccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTc                    <  1:3414316/71‑1 (MQ=255)
                                               ccccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTc                    <  1:433620/71‑1 (MQ=255)
                                               ccccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTc                    <  1:366308/71‑1 (MQ=255)
                                                cccAACTTTTATATAGAGGTTTTTATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGc                            <  1:2268445/62‑1 (MQ=255)
                                                cccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGc                            <  1:4068464/62‑1 (MQ=255)
                                                cccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGc                            <  1:4008056/62‑1 (MQ=255)
                                                cccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGc                            <  1:423622/62‑1 (MQ=255)
                                                 ccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATg                                    >  1:4559737/1‑53 (MQ=255)
                                                 ccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATg                                    >  1:738024/1‑53 (MQ=255)
                                                 ccAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATg                                    >  1:1608259/1‑53 (MQ=255)
                                                  cAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGgt                        <  1:4166097/64‑1 (MQ=255)
                                                   aaCTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGtt                     <  1:4809969/66‑1 (MQ=255)
                                                   aaCTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTAAGGCTGGTGTTCACTTc               >  1:4313338/1‑71 (MQ=255)
                                                       tttATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGt            >  1:2179844/1‑71 (MQ=255)
                                                        ttATATAGAGGTTTTAATCATGGCAACTGTTTCCa                                               >  1:545977/1‑35 (MQ=255)
                                                        ttATATAGAGGTTTTAATCATGGCAACTGTTTCCa                                               >  1:2982399/1‑35 (MQ=255)
                                                                 ggTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGAccc  >  1:2219449/1‑71 (MQ=255)
                                                                 |                                                                      
CCGGGGTGCCCTTTGGGGTCGGTAATATGGGATACGTGGAGGCATAACCCCAACTTTTATATAGAGGTTTTAATCATGGCAACTGTTTCCATGCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCC  >  Exported/168624‑168759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: