Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,611,527 A→G 9.7% L141P (CTT→CCT)  nikB ← nickel transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,611,5270AG 9.7% 442.6 / 28.8 154L141P (CTT→CCT) nikBnickel transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (77/62);  new base G (9/6);  total (86/68)
Fisher's exact test for biased strand distribution p-value = 7.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 157 total aligned reads displayed.

ATTGCAGATACACCGAAAACGCCATCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTCGCGATGGCGCGCCGCCCAGATACCGAGCGG  >  Exported/2611459‑2611597
                                                                    |                                                                      
aTTGCAGATACACCGAAAACGCCATCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGa                                                                      <  1:3852514/71‑1 (MQ=255)
aTTGCAGATACACCGAAAACGCCATCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGa                                                                      <  1:1337813/71‑1 (MQ=255)
   gCAGATACACCGAAAACGCCATCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGaa                                                                     >  1:2370381/1‑69 (MQ=255)
    cAGATACACCGAAAACGCCATCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGaaa                                                                    >  1:2800082/1‑69 (MQ=255)
    cAGATACACCGAAAACGCCATCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGaaa                                                                    >  1:2233848/1‑69 (MQ=255)
     aGATACACCGAAAACGCCATCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAAcgc                                                                 <  1:2916637/71‑1 (MQ=255)
     aGATACACCGAAAACGCCATCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAAcgc                                                                 <  1:1766155/71‑1 (MQ=255)
      gATACACCGAAAACGCCATCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAAcgc                                                                 <  1:348163/70‑1 (MQ=255)
         acacCGAAAACGCCATCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGAt                                                              >  1:2049907/1‑70 (MQ=255)
          cacCGAAAACGCCATCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCGAGAAACGCGATGa                                                            <  1:755874/71‑1 (MQ=255)
           acCGAAAACGCCATCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGaa                                                           <  1:4092986/71‑1 (MQ=255)
             cGAAAACGCCATCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCGAGAAACGCGAtta                                                            <  1:2557219/68‑3 (MQ=255)
             cGAAAACGCCATCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAAc                                                         >  1:431244/1‑71 (MQ=255)
             cGAAAACGCCATCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAAc                                                         >  1:1013650/1‑71 (MQ=255)
             cGAAAACGCCATCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAAc                                                         >  1:3556479/1‑71 (MQ=255)
             cGAAAACGCCATCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAAc                                                         >  1:3932919/1‑71 (MQ=255)
             cGAAAACGCCATCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAAc                                                         >  1:277181/1‑71 (MQ=255)
             cGAAAACGCCATCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAAc                                                         >  1:1123877/1‑71 (MQ=255)
             cGAAAACGCCATCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAAc                                                         >  1:3899007/1‑71 (MQ=255)
                     ccATCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCGAGAAACGCGATGa                                                            <  1:2232614/60‑1 (MQ=255)
                        tCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGaaa                                              >  1:2348923/1‑71 (MQ=255)
                        tCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGaa                                               >  1:3813480/1‑70 (MQ=255)
                              gTAAAAGCGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATc                                            >  1:667708/1‑67 (MQ=255)
                              gTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAAt                                             >  1:2997505/1‑66 (MQ=255)
                              gTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATc                                            >  1:4293140/1‑67 (MQ=255)
                              gTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATc                                            >  1:634750/1‑67 (MQ=255)
                              gTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGc                                        <  1:4876930/71‑1 (MQ=255)
                              gTAAAAACGCCAGCCAGAAGTTAGGCATCGACACCCCAAGAAACGCGATGAAACGTACGGCGAAATCCgg                                         >  1:1525943/1‑70 (MQ=255)
                               tAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACg                                                        <  1:2177284/54‑1 (MQ=255)
                               tAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACg                                                        <  1:3206255/54‑1 (MQ=255)
                               tAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACg                                                        <  1:1343664/54‑1 (MQ=255)
                               tAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACg                                                        <  1:3871289/54‑1 (MQ=255)
                               tAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACg                                                        <  1:1405452/54‑1 (MQ=255)
                               tAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCa                                       <  1:3125399/71‑1 (MQ=255)
                               tAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCa                                       <  1:126703/71‑1 (MQ=255)
                               tAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCa                                       <  1:3853901/71‑1 (MQ=255)
                               tAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCa                                       <  1:2452337/71‑1 (MQ=255)
                               tAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCa                                       <  1:4059465/71‑1 (MQ=255)
                                aaaaaCGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCgg                                         >  1:4263384/1‑68 (MQ=255)
                                aaaaaCGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCgg                                         >  1:1490530/1‑68 (MQ=255)
                                 aaaaCGCCAGCCAGAAGTTAGGCATCGACACGCCGAGAAACGCGATGa                                                            <  1:4637770/48‑1 (MQ=255)
                                 aaaaCGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCa                                       >  1:3947825/1‑69 (MQ=255)
                                 aaaaCGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGa                                     >  1:3973910/1‑71 (MQ=255)
                                   aaCGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACg                                   >  1:2588081/1‑71 (MQ=255)
                                     cGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGt                                 <  1:1031924/71‑1 (MQ=255)
                                     cGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGt                                 <  1:1897425/71‑1 (MQ=255)
                                      gccagccaGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGt                                 <  1:656061/70‑1 (MQ=255)
                                        cagccagAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTcgc                              <  1:5118359/71‑1 (MQ=255)
                                        cagccagAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTcgc                              <  1:1812660/71‑1 (MQ=255)
                                        cagccagAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTcgc                              <  1:3398459/71‑1 (MQ=255)
                                        cagccagAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTcgc                              <  1:3392201/71‑1 (MQ=255)
                                          gccagAAGTTAGGCATCGACACGCCAGGAAACGCGATGAAACg                                                        <  1:571968/43‑1 (MQ=255)
                                          gccagAAGTTAGGCATCGACACGCCAGGAAACGCGATGAAACg                                                        <  1:1591956/43‑1 (MQ=255)
                                          gccagAAGTTAGGCATCGACACGCCAGGAAACGCGATGAAACg                                                        <  1:3448849/43‑1 (MQ=255)
                                          gccagAAGTTAGGCATCGACACGCCAGGAAACGCGATGAAACg                                                        <  1:4035267/43‑1 (MQ=255)
                                          gccagAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTCGCGa                            >  1:4560115/1‑71 (MQ=255)
                                          gccagAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTCGCGa                            >  1:4449573/1‑71 (MQ=255)
                                          gccagAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTCGCGa                            >  1:4244363/1‑71 (MQ=255)
                                            cagAAGTTAGGCATCGACACGCCAGGAAACGCGATGaaa                                                          >  1:4691223/1‑39 (MQ=255)
                                            cagAAGTTAGGCATCGACACGCCAGGAAACGCGATGAAACGTa                                                      >  1:126991/1‑43 (MQ=255)
                                            cagAAGTTAGGCATCGACACGCCAGGAAACGCGATGAAACGTa                                                      >  1:115655/1‑43 (MQ=255)
                                            cagAAGTTAGGCATCGACACGCCAGGAAACGCGATGAAACGTa                                                      >  1:407429/1‑43 (MQ=255)
                                            cagAAGTTAGGCATCGACACGCCAGGAAACGCGATGAAACGTa                                                      >  1:3332518/1‑43 (MQ=255)
                                            cagAAGTTAGGCATCGACACGCCAGGAAACGCGATGAAACGTa                                                      >  1:4476154/1‑43 (MQ=255)
                                            cagAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTAc                                                     <  1:4916711/44‑1 (MQ=255)
                                            cagAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTAc                                                     <  1:3291668/44‑1 (MQ=255)
                                            cagAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTAc                                                     <  1:3035100/44‑1 (MQ=255)
                                            cagAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTAc                                                     <  1:1732823/44‑1 (MQ=255)
                                            cagAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTAc                                                     <  1:1989919/44‑1 (MQ=255)
                                            cagAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTCGCGATg                          <  1:3794764/71‑1 (MQ=255)
                                            cagAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTCGCGATg                          <  1:1036352/71‑1 (MQ=255)
                                            cagAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTCGCGATg                          <  1:2673711/71‑1 (MQ=255)
                                            cagAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTCGCGATg                          <  1:1982779/71‑1 (MQ=255)
                                                 gTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTCGCGATGgcgcg                     >  1:685337/1‑71 (MQ=255)
                                                    aGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTCGCGAt                           >  1:3006062/1‑62 (MQ=255)
                                                    aGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTCGCGAt                           >  1:4644235/1‑62 (MQ=255)
                                                    aGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTCGCGAt                           >  1:2388617/1‑62 (MQ=255)
                                                    aGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTCGCGATGGCGcgccg                  >  1:1451681/1‑71 (MQ=255)
                                                      gCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTcgc                              <  1:3522723/57‑1 (MQ=255)
                                                        aTCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTCGCGATGGCGCGCCGCCCa              <  1:4114344/71‑1 (MQ=255)
                                                         tCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCa                                       <  1:2541349/45‑1 (MQ=255)
                                                         tCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCa                                       <  1:3355631/45‑1 (MQ=255)
                                                         tCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTCGCGATGGCGCGCCGCCCAg             <  1:917118/71‑1 (MQ=255)
                                                          cGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCa                                       <  1:804166/44‑1 (MQ=255)
                                                          cGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTcg                               >  1:4540217/1‑52 (MQ=255)
                                                          cGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTcg                               >  1:1723930/1‑52 (MQ=255)
                                                           gACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGa                                     >  1:261374/1‑45 (MQ=255)
                                                           gACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGa                                     >  1:2389678/1‑45 (MQ=255)
                                                           gACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGa                                     >  1:3452696/1‑45 (MQ=255)
                                                           gACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGa                                     >  1:1485329/1‑45 (MQ=255)
                                                           gACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGa                                     >  1:2269637/1‑45 (MQ=255)
                                                           gACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGa                                     >  1:4295586/1‑45 (MQ=255)
                                                              acGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTCGCGATGGCGCGCCGCCCAGATAcc        >  1:1322864/1‑71 (MQ=255)
                                                                 ccAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGa                                     >  1:1610776/1‑39 (MQ=255)
                                                                 ccAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGa                                     >  1:4897868/1‑39 (MQ=255)
                                                                 ccAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGa                                     >  1:3197792/1‑39 (MQ=255)
                                                                 ccAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGa                                     >  1:1050232/1‑39 (MQ=255)
                                                                 ccAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGa                                     >  1:2670941/1‑39 (MQ=255)
                                                                    aGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTCGCGATGGCGCGCCGCCCAGATACCg       >  1:5039176/1‑66 (MQ=255)
                                                                    aGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTCGCGATGGCGCGCCGCCCAGATACCGAGCgg  >  1:4857585/1‑71 (MQ=255)
                                                                    |                                                                      
ATTGCAGATACACCGAAAACGCCATCACCAGTAAAAACGCCAGCCAGAAGTTAGGCATCGACACGCCAAGAAACGCGATGAAACGTACGGCGAAATCCGGCAGACGGTCGCGATGGCGCGCCGCCCAGATACCGAGCGG  >  Exported/2611459‑2611597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: