Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,749,139 C→G 8.5% T404T (ACC→ACG secY → preprotein translocase membrane subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,749,1390CG 8.5% 424.6 / 23.2 141T404T (ACC→ACGsecYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (74/55);  new base G (4/8);  total (78/63)
Fisher's exact test for biased strand distribution p-value = 1.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.52e-01

Only 100 of 150 total aligned reads displayed.

TTACCTTTATCTGCCTGATCCCGGAGTTCATGCGTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTCAAGTGCAAACTCTGATGATGTCCAGT  >  Exported/2749069‑2749208
                                                                      |                                                                     
ttACCTTTATCTGCCTGATCCCGGAGTTCATGCGTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGAcc                                                                       >  1:1059333/1‑71 (MQ=255)
   ccTTTATCTGCCTGATCCCGGAGTTCATGCGTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTc                                                                     <  1:1199163/70‑1 (MQ=255)
      ttATCTGCCTGATCCCGGAGTTCATGCGTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTg                                                                 >  1:4844591/1‑71 (MQ=255)
       tATCTGCCTGATCCCGGAGTTCATGCGTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACt                                                                  <  1:2613858/69‑1 (MQ=255)
       tATCTGCCTGATCCCGGAGTTCATGCGTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACt                                                                  <  1:4937699/69‑1 (MQ=255)
       tATCTGCCTGATCCCGGAGTTCATGCGTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACt                                                                  <  1:318387/69‑1 (MQ=255)
          cTGCATGATCCCGGAGTTCATGCGTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACt                                                                  <  1:1355905/66‑1 (MQ=255)
              cTGATCCCGGAGTTCATGCGTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCgt                                                         >  1:3277515/1‑71 (MQ=255)
                    ccGGAGTTCATGCGTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCAc                                                                   >  1:2685803/1‑55 (MQ=255)
                    ccGGAGTTCATGCGTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGttgttg                                                    >  1:2680075/1‑70 (MQ=255)
                    ccGGAGTTCATGCGTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGttgttg                                                    <  1:3959419/70‑1 (MQ=255)
                    ccGGAGTTCATGCGTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTtgttgt                                                   >  1:2470578/1‑71 (MQ=255)
                           tCATGCGTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGAt                                             <  1:1511397/70‑1 (MQ=255)
                            cATGCGTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGAt                                             <  1:4131207/69‑1 (MQ=255)
                            cATGCGTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGAt                                             <  1:2935735/69‑1 (MQ=255)
                            cATGCGTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGAt                                             <  1:3242683/69‑1 (MQ=255)
                            cATGCGTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGAt                                             <  1:1659390/69‑1 (MQ=255)
                            cATGCGTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGAt                                             <  1:4420140/69‑1 (MQ=255)
                               gCGTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGAt                                             <  1:4588896/66‑1 (MQ=255)
                                 gTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGAc                                      <  1:3372880/71‑1 (MQ=255)
                                 gTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGAc                                      <  1:623377/71‑1 (MQ=255)
                                 gTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGAc                                      <  1:709671/71‑1 (MQ=255)
                                 gTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGAc                                      <  1:840211/71‑1 (MQ=255)
                                    aTGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATc                                                           >  1:4639277/1‑47 (MQ=255)
                                    aTGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACtt                                    >  1:3521659/1‑70 (MQ=255)
                                      gCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGa                                       >  1:990965/1‑65 (MQ=255)
                                      gCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTa                                  >  1:2485480/1‑70 (MQ=255)
                                      gCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTa                                  >  1:4707745/1‑70 (MQ=255)
                                      gCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTa                                  >  1:5157511/1‑70 (MQ=255)
                                      gCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTa                                  >  1:3750444/1‑70 (MQ=255)
                                      gCAATGAAAGTACCGTTCTACTTAGGTGGGACCTCACTGCt                                                               >  1:1170159/1‑41 (MQ=255)
                                       cAATGAAAGTACCGTTCTACTTCGGTGGGACCTCa                                                                    >  1:15770/1‑35 (MQ=255)
                                       cAATGAAAGTACCGTTCTACTTCGGTGGGACCTCa                                                                    >  1:1239595/1‑35 (MQ=255)
                                       cAATGAAAGTACCGTTCTACTTCGGTGGGACCTCa                                                                    >  1:2857214/1‑35 (MQ=255)
                                       cAATGAAAGTACCGTTCTACTTCGGTGGGACCTCa                                                                    >  1:4927171/1‑35 (MQ=255)
                                       cAATGAAAGTACCGTTCTACTTCGGTGGGACCTCa                                                                    >  1:1331889/1‑35 (MQ=255)
                                       cAATGAAAGTACCGTTCTACTTCGGTGGGACCTCa                                                                    >  1:4003480/1‑35 (MQ=255)
                                       cAATGAAAGTACCGTTCTACTTCGGTGGGACCTCa                                                                    >  1:4175496/1‑35 (MQ=255)
                                          tGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGc                              >  1:1920914/1‑70 (MQ=255)
                                          tGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCt                             >  1:3650084/1‑71 (MQ=255)
                                          tGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCt                             >  1:1740008/1‑71 (MQ=255)
                                          tGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCt                             >  1:608828/1‑71 (MQ=255)
                                          tGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCt                             >  1:4630681/1‑71 (MQ=255)
                                          tGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCt                             >  1:2069657/1‑71 (MQ=255)
                                          tGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTAATGGc                              >  1:4399015/1‑70 (MQ=255)
                                           gAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCt                             >  1:3858092/1‑70 (MQ=255)
                                            aaaGTACCGTTCTACTTCGGTGGGACCTCACTGCtt                                                              >  1:2824878/1‑36 (MQ=255)
                                            aaaGTACCGTTCTACTTCGGTGGGACCTCACTGCtt                                                              >  1:1434510/1‑36 (MQ=255)
                                            aaaGTACCGTTCTACTTCGGTGGGACCTCACTGCtt                                                              >  1:858312/1‑36 (MQ=255)
                                            aaaGTACCGTTCTACTTCGGTGGGACCTCACTGCtt                                                              >  1:2763432/1‑36 (MQ=255)
                                            aaaGTACCGTTCTACTTCGGTGGGACCTCACTGCtt                                                              >  1:1042127/1‑36 (MQ=255)
                                            aaaGTACCGTTCTACTTCGGTGGGACCTCACTGCtt                                                              >  1:5117601/1‑36 (MQ=255)
                                            aaaGTACCGTTCTACTTCGGTGGGACCTCACTGCtt                                                              >  1:1243909/1‑36 (MQ=255)
                                            aaaGTACCGTTCTACTTCGGTGGGACCTCACTGCtt                                                              >  1:2089699/1‑36 (MQ=255)
                                            aaaGTACCGTTCTACTTCGGTGGGACCTCACTGCtt                                                              >  1:68063/1‑36 (MQ=255)
                                            aaaGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTTATTATGGACTTTATGGCTc                            <  1:565287/70‑1 (MQ=255)
                                            aaaGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATTGACTTTATGGCTc                            <  1:853024/70‑1 (MQ=255)
                                            aaaGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTc                            <  1:4756991/70‑1 (MQ=255)
                                            aaaGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTc                            <  1:2028481/70‑1 (MQ=255)
                                            aaaGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTCa                           <  1:5173154/71‑1 (MQ=255)
                                             aaGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTCaa                          <  1:491119/71‑1 (MQ=255)
                                             aaGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTCaa                          <  1:1023758/71‑1 (MQ=255)
                                             aaGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTCaa                          <  1:2319513/71‑1 (MQ=255)
                                             aaGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTCa                           <  1:330823/70‑1 (MQ=255)
                                                tACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGAt                                             <  1:3883139/49‑1 (MQ=255)
                                                tACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGAt                                             <  1:2579221/49‑1 (MQ=255)
                                                tACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACtt                                    <  1:2672345/58‑1 (MQ=255)
                                                tACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACtt                                    <  1:2179014/58‑1 (MQ=255)
                                                tACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACtt                                    <  1:4667503/58‑1 (MQ=255)
                                                tACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACtt                                    <  1:4515746/58‑1 (MQ=255)
                                                tACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACtt                                    <  1:3174251/58‑1 (MQ=255)
                                                 aCCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTAt                                          >  1:1341593/1‑51 (MQ=255)
                                                 aCCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATgg                               >  1:2359240/1‑62 (MQ=255)
                                                 aCCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATgg                               >  1:3108003/1‑62 (MQ=255)
                                                 aCCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATgg                               >  1:2036085/1‑62 (MQ=255)
                                                 aCCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATgg                               >  1:256070/1‑62 (MQ=255)
                                                 aCCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATg                                >  1:2863725/1‑61 (MQ=255)
                                                 aCCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATg                                >  1:3558737/1‑61 (MQ=255)
                                                  ccGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTCAAGTGCa                     <  1:2954252/71‑1 (MQ=255)
                                                    gTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTCAAGTGCaaa                   <  1:1620798/71‑1 (MQ=255)
                                                    gTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTCAAGTGCaaa                   <  1:2649739/71‑1 (MQ=255)
                                                    gTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTCAAGTGCaaa                   <  1:5163669/71‑1 (MQ=255)
                                                    cttCTACTTCGGTGGCACGTCGCTCCTGATCATCGTGGTGGTGACGATGGACTTCATGGCACAAGTGCag                    >  1:3950087/2‑69 (MQ=255)
                                                    cttCTACTTCGGTGGCACGTCGCTCCTGATCATCGTGGTGGTGACGATGGACTTCATGGCACAAGTGCa                     >  1:4182873/2‑69 (MQ=255)
                                                     ttCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTCAAGTGCAAAc                  <  1:904888/71‑1 (MQ=255)
                                                     ttCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTCAAGTGCAAAc                  >  1:1599686/1‑71 (MQ=255)
                                                         aCTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTCAAGTGCAAActct               >  1:3354370/1‑70 (MQ=255)
                                                         aCTTCGGTGGCACGTCGCTCCTTATCATCGTGGTGGTGACGATGGACTTCATGGCACAAGTg                       <  1:4761051/62‑1 (MQ=255)
                                                         aCTTCGGTGGCACGTCGCTCCTGATCATCGTGGTGGTGACGATGGACTTCATGGCACAAGTg                       <  1:3645042/62‑1 (MQ=255)
                                                         aCTTCGGTGGCACGTCGCTCCTGATCATCGTGGTGGTGACGATGGACTTCATGGCACAAGTg                       <  1:975475/62‑1 (MQ=255)
                                                         aCTTCGGTGGCACGTCGCTCCTGATCATCGTGGTGGTGACGATGGACTTCATGGCACAACTg                       <  1:2228080/62‑1 (MQ=255)
                                                             cGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTCAAGTGCAAACTCtgatg           <  1:1951655/70‑1 (MQ=255)
                                                                 gggACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTCAAGTGCAAACTCTGATGATGTc      >  1:3145537/1‑71 (MQ=255)
                                                                 gggACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTCAAGTGCAAACTCTGATGATGTc      >  1:1814316/1‑71 (MQ=255)
                                                                     ccTCACTGCTTATCGTTGTTGTCGTGATTATGGACtt                                    <  1:695637/37‑1 (MQ=255)
                                                                     ccTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTCAAGTGCAAACTCTGATGATGTCcagt  <  1:5183365/71‑1 (MQ=255)
                                                                     ccTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTCAAGTGCAAACTCTGATGATGTCcagt  <  1:2738942/71‑1 (MQ=255)
                                                                     ccTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTCAAGTGCAAACTCTGATGATGTCcagt  <  1:3253437/71‑1 (MQ=255)
                                                                     ccTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTCAAGTGCAAACTCTGATGATGTCcagt  <  1:4866089/71‑1 (MQ=255)
                                                                     ccTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTCAAGTGCAAACTCTGATGATGTCcagt  <  1:3760982/71‑1 (MQ=255)
                                                                      |                                                                     
TTACCTTTATCTGCCTGATCCCGGAGTTCATGCGTGATGCAATGAAAGTACCGTTCTACTTCGGTGGGACCTCACTGCTTATCGTTGTTGTCGTGATTATGGACTTTATGGCTCAAGTGCAAACTCTGATGATGTCCAGT  >  Exported/2749069‑2749208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: