Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,965,091	A→G	10.4%	K191K (AAA→AAG)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,965,091	0	A	G	10.4%	183.5 / 10.9	67	K191K (AAA→AAG)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base A (25/35);  new base G (4/3);  total (29/38)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.43e-01

TTACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTAC  >  Exported/2965036‑2965160                                                        |                                                                      ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                                        <  1:3981556/71‑1 (MQ=255) ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                                        <  1:2187430/71‑1 (MQ=255) ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                                        <  1:4275318/71‑1 (MQ=255) ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                                        <  1:4556448/71‑1 (MQ=255) ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                                        <  1:172204/71‑1 (MQ=255)   aCCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTgg                                                       <  1:346309/70‑1 (MQ=255)   aCCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTgg                                                       <  1:5040416/70‑1 (MQ=255)   aCCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTgg                                                       <  1:3606100/70‑1 (MQ=255)    ccGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAc                                                     >  1:4813304/1‑71 (MQ=255)       cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgcg                                                  >  1:4779475/1‑71 (MQ=255)       cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgcg                                                  >  1:248198/1‑71 (MQ=255)       cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgcg                                                  >  1:3720867/1‑71 (MQ=255)       cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgc                                                   >  1:3699205/1‑70 (MQ=255)       cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcg                                                    >  1:5087612/1‑69 (MQ=255)         aCCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCt                                                <  1:2012054/71‑1 (MQ=255)             tgtaCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgc                                                   <  1:4562786/61‑1 (MQ=255)             tGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGtt                                            <  1:2211737/71‑2 (MQ=255)             tGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTg                                            <  1:1541262/71‑1 (MQ=255)                aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                                         <  1:3661095/71‑1 (MQ=255)                aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                                         <  1:3819659/71‑1 (MQ=255)                aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                                         <  1:4317285/71‑1 (MQ=255)                aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                                         <  1:2133124/71‑1 (MQ=255)                aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                                         <  1:1747669/71‑1 (MQ=255)                    cATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGAc                                      >  1:726518/1‑70 (MQ=255)                    cATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACg                                     >  1:2423087/1‑71 (MQ=255)                    cATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGCAAGGTCTGGa                                                      >  1:4767651/1‑54 (MQ=255)                        ccGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGa                                  <  1:933279/70‑1 (MQ=255)                        ccGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGa                                  <  1:748009/70‑1 (MQ=255)                        ccGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGa                                  <  1:749094/70‑1 (MQ=255)                        ccGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGa                                  <  1:2422454/70‑1 (MQ=255)                            tGATCACCGGCTCGATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGtc                             >  1:5139499/1‑70 (MQ=255)                            tGATCACCGGCTCGATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGtc                             >  1:3765659/1‑70 (MQ=255)                            tGATCACCGGCTCGATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGtc                             >  1:627910/1‑70 (MQ=255)                            tGATCACCGGCTCGATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGtc                             >  1:4654633/1‑70 (MQ=255)                             gATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTgg                            >  1:2511946/1‑71 (MQ=255)                                cACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                              >  1:5088838/1‑50 (MQ=255)                                cACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                              >  1:509576/1‑50 (MQ=255)                                cACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                              >  1:542705/1‑50 (MQ=255)                                  ccGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAgc                       <  1:1572932/71‑1 (MQ=255)                                  ccGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAgc                       <  1:1564036/71‑1 (MQ=255)                                   cggctcgATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGTCGGCAgcg                      <  1:5072468/68‑1 (MQ=255)                                   cggctcgATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGTCGGCAgcg                      <  1:4421560/68‑1 (MQ=255)                                   cggctcgATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGTCGGCAgcg                      <  1:5096278/68‑1 (MQ=255)                                     ccTCTATTCTGGCGAAGAAACTAGCGGAAGGACTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAgcggc                    >  1:1677587/1‑71 (MQ=255)                                       tctATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                >  1:5198074/1‑57 (MQ=255)                                         tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGt                                             <  1:1788533/42‑1 (MQ=255)                                         tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGt                                             <  1:1698747/42‑1 (MQ=255)                                         tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGt                                             <  1:3360109/42‑1 (MQ=255)                                         tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGt                                             <  1:3353852/42‑1 (MQ=255)                                         tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGt                >  1:3431812/1‑71 (MQ=255)                                         tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGt                >  1:3394182/1‑71 (MQ=255)                                           ttCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGa                                       <  1:4703971/46‑1 (MQ=255)                                           ttCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGa                                       <  1:3111659/46‑1 (MQ=255)                                           ttCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGa                                       <  1:2363989/46‑1 (MQ=255)                                           ttCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGtt               <  1:4676491/70‑1 (MQ=255)                                            tCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                              >  1:3567664/1‑38 (MQ=255)                                            tCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                              >  1:752776/1‑38 (MQ=255)                                             cTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTa             >  1:3790149/1‑70 (MQ=255)                                                gCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTAt            <  1:288183/68‑1 (MQ=255)                                                gCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATg           <  1:3369350/69‑1 (MQ=255)                                                 cGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTa             >  1:4314104/1‑66 (MQ=255)                                                 cGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTa             >  1:2190962/1‑66 (MQ=255)                                                 cGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTa             >  1:1118185/1‑66 (MQ=255)                                                 cGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTa             >  1:1580525/1‑66 (MQ=255)                                                 cGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGAGCGTTTa             >  1:1894959/1‑66 (MQ=255)                                                  gaagaaACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTa             <  1:3338671/65‑1 (MQ=255)                                                  gaagaaACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTa             <  1:3469375/65‑1 (MQ=255)                                                      aaaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGtt               <  1:414006/59‑1 (MQ=255)                                                      aaaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGtt               <  1:1211473/59‑1 (MQ=255)                                                       aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                >  1:1756997/1‑41 (MQ=255)                                                       aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                >  1:443967/1‑41 (MQ=255)                                                       aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                >  1:1840212/1‑41 (MQ=255)                                                       aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                >  1:4293125/1‑41 (MQ=255)                                                       aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                >  1:3816621/1‑41 (MQ=255)                                                       aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                >  1:3793051/1‑41 (MQ=255)                                                       aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                >  1:2364113/1‑41 (MQ=255)                                                       aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                >  1:1272662/1‑41 (MQ=255)                                                       aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                >  1:342745/1‑41 (MQ=255)                                                       aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTAc  >  1:2660731/1‑71 (MQ=255)                                                        aCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTAc  >  1:4251476/1‑70 (MQ=255)                                                        aCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTAc  >  1:1433811/1‑70 (MQ=255)                                                        |                                                                      TTACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTAC  >  Exported/2965036‑2965160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑