BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	2,965,099	A→C	6.9%	E194A (GAA→GCA) ‡	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	2,965,099	0	A	C	6.9%	275.1 / 13.9	101	E194A (GAA→GCA) ‡	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand): ref base A (37/57); new base C (4/3); total (41/60)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

Only 100 of 101 total aligned reads displayed.

TTACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGA  >  Exported/2965036‑2965162

ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                                          <  1:2187430/71‑1 (MQ=255)

ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                                          <  1:3981556/71‑1 (MQ=255)

ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                                          <  1:4275318/71‑1 (MQ=255)

ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                                          <  1:4556448/71‑1 (MQ=255)

ttACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTg                                                          <  1:172204/71‑1 (MQ=255)

  aCCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTgg                                                         <  1:5040416/70‑1 (MQ=255)

  aCCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTgg                                                         <  1:3606100/70‑1 (MQ=255)

  aCCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTgg                                                         <  1:346309/70‑1 (MQ=255)

   ccGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAc                                                       >  1:4813304/1‑71 (MQ=255)

      cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgcg                                                    >  1:248198/1‑71 (MQ=255)

      cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgcg                                                    >  1:3720867/1‑71 (MQ=255)

      cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgcg                                                    >  1:4779475/1‑71 (MQ=255)

      cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgc                                                     >  1:3699205/1‑70 (MQ=255)

      cAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcg                                                      >  1:5087612/1‑69 (MQ=255)

        aCCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCt                                                  <  1:2012054/71‑1 (MQ=255)

            tgtaCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGAcgc                                                     <  1:4562786/61‑1 (MQ=255)

            tGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGtt                                              <  1:2211737/71‑2 (MQ=255)

            tGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTg                                              <  1:1541262/71‑1 (MQ=255)

               aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                                           <  1:1747669/71‑1 (MQ=255)

               aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                                           <  1:4317285/71‑1 (MQ=255)

               aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                                           <  1:2133124/71‑1 (MQ=255)

               aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                                           <  1:3661095/71‑1 (MQ=255)

               aCTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATg                                           <  1:3819659/71‑1 (MQ=255)

                   cATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGAc                                        >  1:726518/1‑70 (MQ=255)

                   cATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACg                                       >  1:2423087/1‑71 (MQ=255)

                   cATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGCAAGGTCTGGa                                                        >  1:4767651/1‑54 (MQ=255)

                       ccGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGa                                    <  1:2422454/70‑1 (MQ=255)

                       ccGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGa                                    <  1:933279/70‑1 (MQ=255)

                       ccGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGa                                    <  1:748009/70‑1 (MQ=255)

                       ccGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGa                                    <  1:749094/70‑1 (MQ=255)

                           tGATCACCGGCTCGATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGtc                               >  1:5139499/1‑70 (MQ=255)

                           tGATCACCGGCTCGATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGtc                               >  1:3765659/1‑70 (MQ=255)

                           tGATCACCGGCTCGATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGtc                               >  1:627910/1‑70 (MQ=255)

                           tGATCACCGGCTCGATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGtc                               >  1:4654633/1‑70 (MQ=255)

                            gATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTgg                              >  1:2511946/1‑71 (MQ=255)

                               cACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                                >  1:5088838/1‑50 (MQ=255)

                               cACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                                >  1:509576/1‑50 (MQ=255)

                               cACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                                >  1:542705/1‑50 (MQ=255)

                                 ccGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAgc                         <  1:1564036/71‑1 (MQ=255)

                                 ccGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAgc                         <  1:1572932/71‑1 (MQ=255)

                                  cggctcgATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGTCGGCAgcg                        <  1:5096278/68‑1 (MQ=255)

                                  cggctcgATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGTCGGCAgcg                        <  1:5072468/68‑1 (MQ=255)

                                  cggctcgATCCTGTCCAAGAAGCTGTCGGCGGGCCTGGATGCGCTGGTGATGGACGTGAAGGTCGGCAgcg                        <  1:4421560/68‑1 (MQ=255)

                                    ccTCTATTCTGGCGAAGAAACTAGCGGAAGGACTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAgcggc                      >  1:1677587/1‑71 (MQ=255)

                                      tctATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                  >  1:5198074/1‑57 (MQ=255)

                                        tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGt                                               <  1:1698747/42‑1 (MQ=255)

                                        tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGt                                               <  1:3360109/42‑1 (MQ=255)

                                        tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGt                                               <  1:3353852/42‑1 (MQ=255)

                                        tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGt                                               <  1:1788533/42‑1 (MQ=255)

                                        tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGt                  >  1:3394182/1‑71 (MQ=255)

                                        tATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGt                  >  1:3431812/1‑71 (MQ=255)

                                          ttCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGa                                         <  1:3111659/46‑1 (MQ=255)

                                          ttCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGa                                         <  1:4703971/46‑1 (MQ=255)

                                          ttCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGa                                         <  1:2363989/46‑1 (MQ=255)

                                          ttCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGtt                 <  1:4676491/70‑1 (MQ=255)

                                           tCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                                >  1:752776/1‑38 (MQ=255)

                                           tCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTgg                                                >  1:3567664/1‑38 (MQ=255)

                                            cTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTa               >  1:3790149/1‑70 (MQ=255)

                                               gCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTAt              <  1:288183/68‑1 (MQ=255)

                                               gCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATg             <  1:3369350/69‑1 (MQ=255)

                                                cGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTa               >  1:1118185/1‑66 (MQ=255)

                                                cGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTa               >  1:1580525/1‑66 (MQ=255)

                                                cGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTa               >  1:2190962/1‑66 (MQ=255)

                                                cGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTa               >  1:4314104/1‑66 (MQ=255)

                                                cGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGAGCGTTTa               >  1:1894959/1‑66 (MQ=255)

                                                 gaagaaACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTa               <  1:3469375/65‑1 (MQ=255)

                                                 gaagaaACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTa               <  1:3338671/65‑1 (MQ=255)

                                                     aaaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGtt                 <  1:414006/59‑1 (MQ=255)

                                                     aaaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGtt                 <  1:1211473/59‑1 (MQ=255)

                                                      aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                  >  1:3793051/1‑41 (MQ=255)

                                                      aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                  >  1:2364113/1‑41 (MQ=255)

                                                      aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                  >  1:1840212/1‑41 (MQ=255)

                                                      aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                  >  1:1756997/1‑41 (MQ=255)

                                                      aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                  >  1:3816621/1‑41 (MQ=255)

                                                      aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                  >  1:1272662/1‑41 (MQ=255)

                                                      aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                  >  1:443967/1‑41 (MQ=255)

                                                      aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                  >  1:342745/1‑41 (MQ=255)

                                                      aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGaaa                                  >  1:4293125/1‑41 (MQ=255)

                                                      aaCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTAc    >  1:2660731/1‑71 (MQ=255)

                                                       aCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTAc    >  1:1433811/1‑70 (MQ=255)

                                                       aCTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTAc    >  1:4251476/1‑70 (MQ=255)

                                                        cTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGttt                <  1:1124774/57‑1 (MQ=255)

                                                        cTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGttt                <  1:428748/57‑1 (MQ=255)

                                                        cTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGttt                <  1:2411933/57‑1 (MQ=255)

                                                        cTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGa  <  1:3479313/71‑1 (MQ=255)

                                                         ttGCGGAAGGTCTGGACGCGCTTGTGATGGACGTg                                     <  1:4682206/35‑1 (MQ=255)

                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:2613051/35‑1 (MQ=255)

                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:50677/35‑1 (MQ=255)

                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:5025086/35‑1 (MQ=255)

                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:497479/35‑1 (MQ=255)

                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:3934055/35‑1 (MQ=255)

                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:2117419/35‑1 (MQ=255)

                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:2817060/35‑1 (MQ=255)

                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:477423/35‑1 (MQ=255)

                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:1115289/35‑1 (MQ=255)

                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:2994071/35‑1 (MQ=255)

                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:1561257/35‑1 (MQ=255)

                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:4688227/35‑1 (MQ=255)

                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:3183666/35‑1 (MQ=255)

                                                         ttGCGGAAGGTCTGGACGCGCTGGTGATGGACGTg                                     <  1:3367068/35‑1 (MQ=255)

TTACCGCAACCGTGGACTCCATCCCGCTGATCACCGCCTCTATTCTGGCGAAGAAACTTGCGGAAGGTCTGGACGCGCTGGTGATGGACGTGAAAGTGGGTAGCGGCGCGTTTATGCCGACCTACGA  >  Exported/2965036‑2965162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`