Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 168,781 A→C 16.9% K28T (AAG→ACG) ‡ rpsB → 30S ribosomal subunit protein S2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported168,7810AC16.9% 156.4 / 37.9 83K28T (AAG→ACG) ‡rpsB30S ribosomal subunit protein S2
Reads supporting (aligned to +/- strand):  ref base A (33/36);  new base C (6/8);  total (39/44)
Fisher's exact test for biased strand distribution p-value = 7.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01

GCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATG  >  Exported/168716‑168845
                                                                 |                                                                
gcgaGACATGCTCAAGGCTGGTGTTAACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGt                                                             >  1:1938177/1‑71 (MQ=255)
 cgcgACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGtt                                                            >  1:331490/1‑71 (MQ=255)
 cgcgACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGtt                                                            >  1:2080191/1‑71 (MQ=255)
   cgACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCa                                                          >  1:3721297/1‑71 (MQ=255)
   cgACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCa                                                          >  1:2351522/1‑71 (MQ=255)
       aTGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCtt                                                      <  1:3924032/71‑1 (MQ=255)
        tGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTc                                                     <  1:4992802/71‑1 (MQ=255)
          cTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCgg                                                   <  1:3843249/71‑1 (MQ=255)
          cTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCgg                                                   <  1:4024852/71‑1 (MQ=255)
          cTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCgg                                                   <  1:181749/71‑1 (MQ=255)
               ggCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCg                                                    >  1:4026613/1‑65 (MQ=255)
                     tgtTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAAct                                        <  1:2321346/71‑2 (MQ=255)
                     tgtTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACa                                        <  1:1047860/71‑1 (MQ=255)
                         cACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGt                                    >  1:2150660/1‑71 (MQ=255)
                          aCTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGtt                                   <  1:4969061/71‑1 (MQ=255)
                          aCTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGtt                                   <  1:3635253/71‑1 (MQ=255)
                          aCTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGtt                                   <  1:2827670/71‑1 (MQ=255)
                          aCTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGtt                                   <  1:2762509/71‑1 (MQ=255)
                          aCTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGtt                                   <  1:4640291/71‑1 (MQ=255)
                          aCTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGtt                                   <  1:925167/71‑1 (MQ=255)
                          aCTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGtt                                   <  1:4006017/71‑1 (MQ=255)
                          aCTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGtt                                   <  1:3984658/71‑1 (MQ=255)
                              cGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCtt                                                      >  1:1425125/1‑48 (MQ=255)
                                 tCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGtt                                   <  1:3640484/64‑1 (MQ=255)
                                 ccACCAGACCCGTTTCTGGAACCCAAAGATGGCGCCGTTCATCTTCGGtc                                                 <  1:3691794/49‑2 (MQ=255)
                                 ccACCAGACCCGTTTCTGGAACCCAAAGATGGCGCCGTTCATCTTCGGtc                                                 <  1:2411383/49‑2 (MQ=255)
                                 ccACCAGACCCGTTTCTGGAACCCAAAGATGGCGCCGTTCATCTTCGGtc                                                 <  1:4473186/49‑2 (MQ=255)
                                 ccACCAGACCCGTTTCTGGAACCCAAAGATGGCGCCGTTCATCTTCGGtc                                                 <  1:3183768/49‑2 (MQ=255)
                                 ccACCAGACCCGTTTCTGGAACCCAAAGATGGCGCCGTTCATCTTCGGtc                                                 <  1:3278474/49‑2 (MQ=255)
                                   aCCAGACCCGTTTCTGGAACCCAAAGATGGCGCCGTTCATCTTCg                                                    <  1:4524942/45‑1 (MQ=255)
                                   aCCAGACCCGTTTCTGGAACCCAAAGATGGCGCCGTTCATCTTCg                                                    <  1:345409/45‑1 (MQ=255)
                                   aCCAGACCCGTTTCTGGAACCCAAAGATGGCGCCGTTCATCTTCg                                                    <  1:4117064/45‑1 (MQ=255)
                                   aCCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACatcatc                          <  1:1405846/71‑1 (MQ=255)
                                      aGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAAc                       >  1:3284444/1‑71 (MQ=255)
                                      aGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAAc                       >  1:4709798/1‑71 (MQ=255)
                                             ttACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatca                            <  1:5164079/59‑1 (MQ=255)
                                             ttACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTg                   <  1:619016/68‑1 (MQ=255)
                                             ttACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTg                   <  1:3305117/68‑1 (MQ=255)
                                             ttACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTg                   <  1:1815441/68‑1 (MQ=255)
                                             ttACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTg                   <  1:949097/68‑1 (MQ=255)
                                              ttcTGGAACCCAAAGATGGCGCCGTTCATCTTCGGTCATCGCAACAAGATCCACATCATCAACCTGgaa                 >  1:3345596/3‑68 (MQ=255)
                                              ttcTGGAACCCAAAGATGGCGCCGTTCATCTTCGGTCATCGCAACAAGATCCACATCATCAACCTGgaa                 >  1:1957858/3‑68 (MQ=255)
                                              ttcTGGAACCCAAAGATGGCGCCGTTCATCTTCGGTCATCGCAACAAGATCCACATCATCAACCTGgaa                 >  1:3153511/3‑68 (MQ=255)
                                              ttcTGGAACCCAAAGATGGCGCCGTTCATCTTCGGTCATCGCAACAAGATCCACATCATCAACCTGgaa                 >  1:613621/3‑68 (MQ=255)
                                              ttcTGGAACCCAAAGATGGCGCCGTTCATCTTCGGTCATCGCAACAAGATCCACATCATCAACCTGgaa                 >  1:3981477/3‑68 (MQ=255)
                                              ttcTGGAACCCAAAGATGGCGCCGTTCATCTTCGGTCATCGCAACAAGATCCACATCATCAACCTGgaa                 >  1:3975713/3‑68 (MQ=255)
                                               aCTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAAc                       >  1:4794908/1‑62 (MQ=255)
                                               aCTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGaa               >  1:4375234/1‑70 (MQ=255)
                                               aCTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGaa               >  1:4675061/1‑70 (MQ=255)
                                                   gAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAAc            <  1:994371/69‑1 (MQ=255)
                                                   gAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAAc            <  1:2231271/69‑1 (MQ=255)
                                                   gAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAAc            <  1:2002149/69‑1 (MQ=255)
                                                   gAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAAc            <  1:3781457/69‑1 (MQ=255)
                                                   gAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAAc            <  1:3776523/69‑1 (MQ=255)
                                                   gAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAAc            <  1:2006176/69‑1 (MQ=255)
                                                   gAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTg          >  1:2662749/1‑71 (MQ=255)
                                                   gAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTg          >  1:3133820/1‑71 (MQ=255)
                                                   gAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTg          >  1:3621608/1‑71 (MQ=255)
                                                   gAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTg          >  1:3371208/1‑71 (MQ=255)
                                                    aaCCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGaaa              <  1:4648313/66‑1 (MQ=255)
                                                    aaCCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGaaa              <  1:3869276/66‑1 (MQ=255)
                                                    aaCCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGt         <  1:4215555/71‑1 (MQ=255)
                                                    aaCCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGt         <  1:591818/71‑1 (MQ=255)
                                                    aaCCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGt         <  1:3921620/71‑1 (MQ=255)
                                                     aCCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGt         <  1:2549488/70‑1 (MQ=255)
                                                      cccGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTa        >  1:2329402/1‑70 (MQ=255)
                                                      cccGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTa        >  1:1064246/1‑70 (MQ=255)
                                                      cccGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTa        >  1:3796152/1‑70 (MQ=255)
                                                         gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatc                             >  1:2604352/1‑46 (MQ=255)
                                                         gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatc                             >  1:433530/1‑46 (MQ=255)
                                                         gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatc                             >  1:4916655/1‑46 (MQ=255)
                                                         gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatc                             >  1:3793428/1‑46 (MQ=255)
                                                         gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatc                             >  1:5056753/1‑46 (MQ=255)
                                                         gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatc                             >  1:1653585/1‑46 (MQ=255)
                                                         gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatc                             >  1:1522768/1‑46 (MQ=255)
                                                         gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatc                             >  1:1159104/1‑46 (MQ=255)
                                                         gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCAcatc                             >  1:3954565/1‑46 (MQ=255)
                                                         gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACatcatc                          <  1:757374/49‑1 (MQ=255)
                                                         gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACatcatc                          >  1:945975/1‑49 (MQ=255)
                                                         gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGa    >  1:3355882/1‑71 (MQ=255)
                                                         gAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGa    >  1:1157110/1‑71 (MQ=255)
                                                           aaaTGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATg  >  1:4179055/1‑71 (MQ=255)
                                                            aaTGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAAc            <  1:1896171/60‑1 (MQ=255)
                                                                 |                                                                
GCGCGACATGCTCAAGGCTGGTGTTCACTTCGGTCACCAGACCCGTTACTGGAACCCGAAAATGAAGCCGTTCATCTTCGGTGCGCGTAACAAAGTTCACATCATCAACCTTGAGAAAACTGTACCGATG  >  Exported/168716‑168845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: