| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 30,369 | T→C | 7.0% | D625D (GAT→GAC) | carB → | carbamoyl‑phosphate synthase large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 30,369 | 0 | T | C | 7.0% | 455.5 / 18.3 | 156 | D625D (GAT→GAC) | carB | carbamoyl‑phosphate synthase large subunit |
| Reads supporting (aligned to +/- strand): ref base T (50/95); new base C (5/6); total (55/101) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01 | |||||||||||
Only 100 of 159 total aligned reads displayed.
CCGGAAACCGTCTCCACCGACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCC > Exported/30301‑30434 | ccGGAAACCGTCTCCACCGACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGAtgt > 1:1589744/1‑71 (MQ=255) cGGAAACCGTCTCCACCGACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGAtgt > 1:875817/1‑70 (MQ=255) cGGAAACCGTCTCCACCGACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGAtgt > 1:566132/1‑70 (MQ=255) aaCCGTCTCCACCGACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTg > 1:3913314/1‑70 (MQ=255) cGTCTCCACCGACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGaaa > 1:1828321/1‑71 (MQ=255) cACCGACTATGACACCTCCGACCGCCTGTACTTCGAGCCGGTGACCCTCGAGGACGTCCTTGAGATCGtcc > 1:4110755/1‑69 (MQ=255) cACCGACTATGACACCTCCGACCGCCTGTACTTCGAGCCGGTGACCCTCGAGGACGTCCTTGAGATCGtcc > 1:1493809/1‑69 (MQ=255) cACCGACTATGACACCTCCGACCGCCTGTACTTCGAGCCGGTGACCCTCGAGGACGTCCTTGAGATCGtcc > 1:3446577/1‑69 (MQ=255) ccGACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCg > 1:717075/1‑70 (MQ=255) ccGACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCg > 1:4619716/1‑70 (MQ=255) ccGACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCg > 1:2371724/1‑70 (MQ=255) gACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTAt > 1:119019/1‑71 (MQ=255) aCTACGACACTTCCGACCGCCTCTACTTCGAGCCTGTAACTCTGGAAGATGTGCTGGa < 1:4574019/58‑1 (MQ=255) aCTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGa < 1:2794747/58‑1 (MQ=255) aCTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGa < 1:873866/58‑1 (MQ=255) aCTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATc < 1:2628490/71‑1 (MQ=255) aCTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATc < 1:529553/71‑1 (MQ=255) aCTAAGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGa < 1:1998514/58‑1 (MQ=255) cGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCgag > 1:2468010/1‑70 (MQ=255) cGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCgag > 1:2295778/1‑70 (MQ=255) cGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCgag > 1:630203/1‑70 (MQ=255) cGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCga < 1:3721724/69‑1 (MQ=255) cGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCga < 1:43048/69‑1 (MQ=255) cGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCga < 1:1000888/69‑1 (MQ=255) cGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCga < 1:3515319/69‑1 (MQ=255) acacTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGaa < 1:41109/70‑1 (MQ=255) ttCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGaa < 1:437476/66‑1 (MQ=255) ttCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGaa < 1:2696476/66‑1 (MQ=255) ttCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGaa < 1:4663375/66‑1 (MQ=255) ttCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGaa < 1:185835/66‑1 (MQ=255) ttCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCg > 1:2883235/1‑70 (MQ=255) ccGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGa > 1:8403/1‑69 (MQ=255) cGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGaaa < 1:4029123/70‑1 (MQ=255) cGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGaaa < 1:2019309/70‑1 (MQ=255) aCCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGc < 1:3003271/71‑1 (MQ=255) cGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATc < 1:1521896/54‑1 (MQ=255) gCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGtt < 1:876596/71‑1 (MQ=255) ctctACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCgag > 1:2333604/1‑54 (MQ=255) ctctACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGaaa > 1:324747/1‑63 (MQ=255) ctctACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGaaa > 1:1058404/1‑63 (MQ=255) ctctACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGaaa > 1:2702704/1‑63 (MQ=255) tctACTTCGAGCCGGTAACTCTGGAATATGTGCTGGAAATCGTGCGTATCGAGAAGCCGa < 1:3137385/60‑1 (MQ=255) tctACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGa < 1:3601728/60‑1 (MQ=255) tctACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGa < 1:1599589/60‑1 (MQ=255) tctACTTCGAACCGGTAACGCTGGAAGACGTGCTGGAAATTGTGCGTATTGAGAAGCCGAAAgg < 1:334480/64‑1 (MQ=255) tctACTTCGAACCGGTAACGCTGGAAGACGTGCTGGAAATTGTGCGTATTGAGAAGCCGAAAgg < 1:2192558/64‑1 (MQ=255) tctACTTCGAACCGGTAACGCTGGAAGACGTGCTGGAAATTGTGCGTATTGAGAAGCCGAAAgg < 1:215911/64‑1 (MQ=255) ctACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGt > 1:1741771/1‑66 (MQ=255) ctACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGt > 1:3320434/1‑66 (MQ=255) ctACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAAACGTGCGTATCg > 1:4563378/1‑50 (MQ=255) tACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTAt > 1:4509580/1‑68 (MQ=255) tACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTAt > 1:4268532/1‑68 (MQ=255) tACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTAt > 1:4470064/1‑68 (MQ=255) aCTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATc > 1:3030803/1‑47 (MQ=255) aCTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATc > 1:4603084/1‑47 (MQ=255) aCTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATc > 1:5108860/1‑47 (MQ=255) aCTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATc > 1:3670426/1‑47 (MQ=255) aCTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATc > 1:1231173/1‑47 (MQ=255) cTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTc < 1:4808171/70‑1 (MQ=255) cTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTc < 1:3168067/70‑1 (MQ=255) cGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAg < 1:461911/70‑1 (MQ=255) cGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAg < 1:3773689/70‑1 (MQ=255) cGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAg > 1:1261094/1‑70 (MQ=255) cGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAg < 1:3286450/70‑1 (MQ=255) cGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAg > 1:1386037/1‑70 (MQ=255) cGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAg < 1:4380947/70‑1 (MQ=255) cGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAg > 1:4154762/1‑70 (MQ=255) cGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAg < 1:1609676/70‑1 (MQ=255) ggTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGtt < 1:2488571/55‑1 (MQ=255) ggTAACTCTGGAAGATGTGCGGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGtt < 1:3116425/55‑1 (MQ=255) gTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCga < 1:989426/38‑1 (MQ=255) gTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCga < 1:4498561/38‑1 (MQ=255) gTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCga < 1:2578629/38‑1 (MQ=255) gTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCga < 1:2128804/38‑1 (MQ=255) gTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCga < 1:1415677/38‑1 (MQ=255) gTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCga < 1:4067624/38‑1 (MQ=255) gTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCga < 1:1228526/38‑1 (MQ=255) gTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCga < 1:3311486/38‑1 (MQ=255) tAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCga < 1:2940560/37‑1 (MQ=255) aaCTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGt > 1:2288848/1‑70 (MQ=255) aCTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCa < 1:2228426/71‑1 (MQ=255) aCTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCa < 1:2866523/71‑1 (MQ=255) aCTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCa < 1:168498/71‑1 (MQ=255) ctctGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTc < 1:3892882/56‑1 (MQ=255) ctctGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTc < 1:2330643/56‑1 (MQ=255) ctctGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTc < 1:1300714/56‑1 (MQ=255) ctctGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTc < 1:4010476/56‑1 (MQ=255) ctctGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCa < 1:2823145/70‑1 (MQ=255) gctGGAAGACGTGCTGGAAATTGTGCGTATTGAGAAGCCGAAAgg < 1:2680432/44‑1 (MQ=37) ctGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTAt > 1:2087090/1‑50 (MQ=255) ctGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTAt > 1:3776964/1‑50 (MQ=255) ctGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTAt > 1:336244/1‑50 (MQ=255) tGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGa < 1:4894627/69‑1 (MQ=255) tGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGa < 1:1358594/69‑1 (MQ=255) gAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGAcccc < 1:4644880/71‑1 (MQ=255) gATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAg < 1:4779134/37‑1 (MQ=255) gATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAg < 1:3647140/37‑1 (MQ=255) gATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAg < 1:1464616/37‑1 (MQ=255) gATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAg < 1:2197018/37‑1 (MQ=255) gATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAg < 1:1169902/37‑1 (MQ=255) | CCGGAAACCGTCTCCACCGACTACGACACTTCCGACCGCCTCTACTTCGAGCCGGTAACTCTGGAAGATGTGCTGGAAATCGTGCGTATCGAGAAGCCGAAAGGCGTTATCGTCCAGTACGGCGGTCAGACCCC > Exported/30301‑30434 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |