| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 385,804 | G→C | 7.9% | V338V (GTG→GTC) | cstA → | carbon starvation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 385,804 | 0 | G | C | 7.9% | 239.3 / 8.4 | 76 | V338V (GTG→GTC) | cstA | carbon starvation protein |
| Reads supporting (aligned to +/- strand): ref base G (34/36); new base C (2/4); total (36/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCT > Exported/385743‑385874 | gCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCt < 1:586759/69‑1 (MQ=255)gCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCt < 1:4652632/69‑1 (MQ=255) ccGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCTGTGTCTGGCTTCCa < 1:1446556/71‑1 (MQ=255) ccGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCa < 1:1427593/71‑1 (MQ=255) ccGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCa < 1:4212524/71‑1 (MQ=255) cGGTATGGACCGGTAACCTGTTCCCGTTCATGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCAt > 1:4729444/1‑71 (MQ=255) ggTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATg < 1:4664225/71‑1 (MQ=255) aTGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcgc > 1:338770/1‑71 (MQ=255) aTGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcgc > 1:3233171/1‑71 (MQ=255) aTGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcgc > 1:2293883/1‑71 (MQ=255) aTGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcgc > 1:5124392/1‑71 (MQ=255) aTGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcgc > 1:4593394/1‑71 (MQ=255) tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATg < 1:3573833/67‑1 (MQ=255) tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATg < 1:3341666/67‑1 (MQ=255) tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATg < 1:3253962/67‑1 (MQ=255) tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATg < 1:2172157/67‑1 (MQ=255) tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATg < 1:1916734/67‑1 (MQ=255) tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATg < 1:1708200/67‑1 (MQ=255) tGGACCGGTAACATGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATg < 1:2504617/67‑1 (MQ=255) ggACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATg < 1:5199712/66‑1 (MQ=255) gACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcg > 1:3877731/1‑67 (MQ=255) gACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTg < 1:3259093/70‑1 (MQ=255) aCCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGa > 1:2782091/1‑70 (MQ=255) aCCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt > 1:2420182/1‑71 (MQ=255) aCCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt > 1:549291/1‑71 (MQ=255) aCCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt > 1:279950/1‑71 (MQ=255) cGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCTCGGTGTCTGGCTTCCa < 1:3458365/60‑1 (MQ=255) ggTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAtctc < 1:2254526/71‑1 (MQ=255) gTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAtctc < 1:647515/70‑1 (MQ=255) gTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATctct > 1:1815246/1‑71 (MQ=255) gTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATctct > 1:4124511/1‑71 (MQ=255) gTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATctct > 1:4263547/1‑71 (MQ=255) cGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGc < 1:527102/41‑1 (MQ=255) cGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGc < 1:1670049/41‑1 (MQ=255) cGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGc < 1:4435936/41‑1 (MQ=255) cttCCTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCACGCGCTGATCTCGTCGGGCACCACGc > 1:4431645/2‑71 (MQ=255) cttCCTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCACGCGCTGATCTCGTCGGGCACCACGc > 1:2451785/2‑71 (MQ=255) ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCt > 1:2542423/1‑36 (MQ=255) ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCtt > 1:4088896/1‑41 (MQ=255) ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCtt > 1:3847013/1‑41 (MQ=255) ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCtt > 1:891711/1‑41 (MQ=255) ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCtt > 1:1908714/1‑41 (MQ=255) ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcg < 1:382769/48‑1 (MQ=255) ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcg < 1:679302/48‑1 (MQ=255) ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcg < 1:4589741/48‑1 (MQ=255) ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcg < 1:4726845/48‑1 (MQ=255) ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATgcg < 1:2896013/48‑1 (MQ=255) ccTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCg > 1:3810118/1‑70 (MQ=255) ccTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCg > 1:3400471/1‑70 (MQ=255) ccTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCACGCGCTGAt < 1:1897151/51‑1 (MQ=19) ccTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCACGCGCTGAt < 1:3124818/51‑1 (MQ=19) ccTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCACGCGCTGAt < 1:5014864/51‑1 (MQ=19) tGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCg > 1:4142249/1‑68 (MQ=255) tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg < 1:3288923/56‑1 (MQ=255) tATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCa > 1:1730582/1‑36 (MQ=255) tATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCa > 1:1367795/1‑36 (MQ=255) tATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg > 1:1055302/1‑54 (MQ=255) tATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGa > 1:2066375/1‑56 (MQ=255) tATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGa > 1:1204589/1‑56 (MQ=255) tATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGa > 1:1016631/1‑56 (MQ=255) cACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTgg > 1:1807834/1‑71 (MQ=255) aCCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGc > 1:3757847/1‑71 (MQ=255) aCCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGc > 1:2577401/1‑71 (MQ=255) ccATCGCCTGCGGCGCCGTCTCGGGCTTCCACGCGCTGATCTCGTCGGGCACCACGCCCAAGATGCtcg < 1:754585/69‑3 (MQ=255) cATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTg < 1:519833/37‑1 (MQ=255) tCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaac > 1:4112289/1‑71 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:4240537/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:5030359/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:4238302/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:3601303/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:3289528/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:390373/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg > 1:3509276/1‑70 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:5012806/70‑1 (MQ=255) gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg < 1:2187567/70‑1 (MQ=255) ggTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGt > 1:1695415/1‑71 (MQ=255) gtCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCt > 1:1330233/1‑71 (MQ=255) | GCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGGCAGGCGTGCT > Exported/385743‑385874 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |