Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 710,036 G→C 17.3% intergenic (‑162/+99) yceJ ← / ← yceO predicted cytochrome b561/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported710,0360GC17.3% 122.3 / 18.6 52intergenic (‑162/+99)yceJ/yceOpredicted cytochrome b561/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (20/23);  new base C (4/5);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

TTCAATAATTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAA  >  Exported/709972‑710105
                                                                |                                                                     
ttCAATAATTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCt                                                                    <  1:2006252/68‑1 (MQ=255)
 tCAATAATTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCt                                                                    <  1:1880417/67‑1 (MQ=255)
   aataatTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGa                                                               >  1:2126073/1‑70 (MQ=255)
   aataatTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAg                                                              >  1:2796381/1‑71 (MQ=255)
       atTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGc                                                           <  1:5112651/70‑1 (MQ=255)
       atTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCt                                                          <  1:4283841/71‑1 (MQ=255)
               ttAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGt                                                        <  1:2782867/65‑1 (MQ=255)
               ttAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGt                                                        <  1:3374947/65‑1 (MQ=255)
                  ataatGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAgg                                               <  1:3901896/71‑1 (MQ=255)
                  ataatGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAgg                                               <  1:2612838/71‑1 (MQ=255)
                  ataatGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAgg                                               <  1:2925708/71‑1 (MQ=255)
                       gAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGaataa                                          >  1:1986808/1‑71 (MQ=255)
                        aaaGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGaata                                           >  1:4771741/1‑69 (MQ=255)
                          aGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGt                                                                 <  1:4497772/45‑1 (MQ=255)
                          aGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGt                                                                 <  1:3233958/45‑1 (MQ=255)
                            aaTCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGaa                                             <  1:1031990/63‑1 (MQ=255)
                                ttCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGt                                                        <  1:212990/48‑1 (MQ=255)
                                   aTAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAtt                              >  1:3057822/1‑71 (MQ=255)
                                   aTAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAtt                              <  1:2517586/71‑1 (MQ=255)
                                   aTAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAtt                              >  1:1948864/1‑71 (MQ=255)
                                     aaGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAgg                                               <  1:3654907/52‑1 (MQ=255)
                                     aaGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAtttt                            >  1:4267481/1‑71 (MQ=255)
                                      aGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAttttt                           >  1:3919562/1‑71 (MQ=255)
                                      aGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAttttt                           >  1:2999409/1‑71 (MQ=255)
                                      aGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAttttt                           <  1:1171786/71‑1 (MQ=255)
                                      aGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAtttt                            >  1:4025461/1‑70 (MQ=255)
                                        tttAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCa                         <  1:4179995/71‑1 (MQ=255)
                                        tttAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCa                         <  1:1035620/71‑1 (MQ=255)
                                        tttAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCa                         <  1:2357741/71‑1 (MQ=255)
                                        tttAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCa                         <  1:1497317/71‑1 (MQ=255)
                                                  tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAt                               >  1:3048406/1‑55 (MQ=255)
                                                  tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAt                               >  1:3478854/1‑55 (MQ=255)
                                                  tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAt                               >  1:3607620/1‑55 (MQ=255)
                                                  tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAt                               >  1:5026125/1‑55 (MQ=255)
                                                    aGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTACATGaaa             <  1:672418/71‑1 (MQ=255)
                                                    aGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGaaa             <  1:999210/71‑1 (MQ=255)
                                                    aGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGaaa             <  1:4633094/71‑1 (MQ=255)
                                                      cATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg           >  1:4060223/1‑71 (MQ=255)
                                                       accAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg           >  1:3190029/3‑70 (MQ=255)
                                                       accAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg           >  1:3009245/3‑70 (MQ=255)
                                                       accAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg           >  1:2914268/3‑70 (MQ=255)
                                                       accAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAg            >  1:2008248/3‑69 (MQ=255)
                                                        tCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg           >  1:4128032/1‑69 (MQ=255)
                                                        ccAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGga         <  1:4337496/70‑1 (MQ=255)
                                                        ccAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGga         <  1:4623873/70‑1 (MQ=255)
                                                        ccAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGga         <  1:1123524/70‑1 (MQ=255)
                                                        ccAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTACACGCTAAATGAAAGGga         <  1:4253674/70‑1 (MQ=255)
                                                        ccAGACATCTCTGATCAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGga         <  1:4721577/70‑1 (MQ=255)
                                                          agagATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg           >  1:1713701/1‑67 (MQ=255)
                                                          agagATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg           >  1:17136/1‑67 (MQ=255)
                                                             gaTGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACg                       >  1:1345833/1‑52 (MQ=255)
                                                             gaTGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACg                       >  1:519359/1‑52 (MQ=255)
                                                                gTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCTCGCTAAATGAAAGGGAGATTaaa  >  1:4769643/1‑70 (MQ=255)
                                                                gTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTaaa  >  1:1521298/1‑70 (MQ=255)
                                                                gTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTaaa  >  1:1289129/1‑70 (MQ=255)
                                                                |                                                                     
TTCAATAATTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAA  >  Exported/709972‑710105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: