Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 710,041 G→A 15.5% intergenic (‑167/+94) yceJ ← / ← yceO predicted cytochrome b561/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported710,0410GA15.5% 140.4 / 17.0 58intergenic (‑167/+94)yceJ/yceOpredicted cytochrome b561/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/26);  new base A (4/5);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

AATAATTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATA  >  Exported/709975‑710107
                                                                  |                                                                  
aataatTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGa                                                                 >  1:2126073/1‑70 (MQ=255)
aataatTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAg                                                                >  1:2796381/1‑71 (MQ=255)
    atTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGc                                                             <  1:5112651/70‑1 (MQ=255)
    atTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCt                                                            <  1:4283841/71‑1 (MQ=255)
            ttAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGt                                                          <  1:3374947/65‑1 (MQ=255)
            ttAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGt                                                          <  1:2782867/65‑1 (MQ=255)
               ataatGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAgg                                                 <  1:2925708/71‑1 (MQ=255)
               ataatGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAgg                                                 <  1:3901896/71‑1 (MQ=255)
               ataatGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAgg                                                 <  1:2612838/71‑1 (MQ=255)
                    gAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGaataa                                            >  1:1986808/1‑71 (MQ=255)
                     aaaGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGaata                                             >  1:4771741/1‑69 (MQ=255)
                       aGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGt                                                                   <  1:4497772/45‑1 (MQ=255)
                       aGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGt                                                                   <  1:3233958/45‑1 (MQ=255)
                         aaTCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGaa                                               <  1:1031990/63‑1 (MQ=255)
                             ttCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGt                                                          <  1:212990/48‑1 (MQ=255)
                                aTAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAtt                                >  1:1948864/1‑71 (MQ=255)
                                aTAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAtt                                <  1:2517586/71‑1 (MQ=255)
                                aTAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAtt                                >  1:3057822/1‑71 (MQ=255)
                                  aaGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAgg                                                 <  1:3654907/52‑1 (MQ=255)
                                  aaGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAtttt                              >  1:4267481/1‑71 (MQ=255)
                                   aGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAttttt                             >  1:3919562/1‑71 (MQ=255)
                                   aGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAttttt                             >  1:2999409/1‑71 (MQ=255)
                                   aGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAttttt                             <  1:1171786/71‑1 (MQ=255)
                                   aGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAtttt                              >  1:4025461/1‑70 (MQ=255)
                                     tttAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCa                           <  1:2357741/71‑1 (MQ=255)
                                     tttAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCa                           <  1:4179995/71‑1 (MQ=255)
                                     tttAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCa                           <  1:1497317/71‑1 (MQ=255)
                                     tttAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCa                           <  1:1035620/71‑1 (MQ=255)
                                               tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAt                                 >  1:3048406/1‑55 (MQ=255)
                                               tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAt                                 >  1:5026125/1‑55 (MQ=255)
                                               tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAt                                 >  1:3478854/1‑55 (MQ=255)
                                               tCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAAt                                 >  1:3607620/1‑55 (MQ=255)
                                                 aGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTACATGaaa               <  1:672418/71‑1 (MQ=255)
                                                 aGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGaaa               <  1:999210/71‑1 (MQ=255)
                                                 aGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGaaa               <  1:4633094/71‑1 (MQ=255)
                                                   cATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg             >  1:4060223/1‑71 (MQ=255)
                                                    accAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg             >  1:3190029/3‑70 (MQ=255)
                                                    accAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg             >  1:3009245/3‑70 (MQ=255)
                                                    accAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg             >  1:2914268/3‑70 (MQ=255)
                                                    accAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAg              >  1:2008248/3‑69 (MQ=255)
                                                     tCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg             >  1:4128032/1‑69 (MQ=255)
                                                     ccAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGga           <  1:1123524/70‑1 (MQ=255)
                                                     ccAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGga           <  1:4623873/70‑1 (MQ=255)
                                                     ccAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGga           <  1:4337496/70‑1 (MQ=255)
                                                     ccAGACATCTCTGATGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTACACGCTAAATGAAAGGga           <  1:4253674/70‑1 (MQ=255)
                                                     ccAGACATCTCTGATCAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGga           <  1:4721577/70‑1 (MQ=255)
                                                       agagATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg             >  1:1713701/1‑67 (MQ=255)
                                                       agagATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAgg             >  1:17136/1‑67 (MQ=255)
                                                          gaTGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACg                         >  1:1345833/1‑52 (MQ=255)
                                                          gaTGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACg                         >  1:519359/1‑52 (MQ=255)
                                                             gTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCTCGCTAAATGAAAGGGAGATTaaa    >  1:4769643/1‑70 (MQ=255)
                                                             gTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTaaa    >  1:1521298/1‑70 (MQ=255)
                                                             gTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTaaa    >  1:1289129/1‑70 (MQ=255)
                                                              tCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAg              <  1:1842657/59‑1 (MQ=255)
                                                              tCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAg              <  1:1715134/59‑1 (MQ=255)
                                                              tCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAg              <  1:2361190/59‑1 (MQ=255)
                                                              tCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAg              <  1:525250/59‑1 (MQ=255)
                                                              tCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAata  <  1:385645/71‑1 (MQ=255)
                                                                  |                                                                  
AATAATTTTGAATTAATAATGAAAGAATCTTCATAAGTTTAGTGGGTTCAGCATCAGAGATGTCTGGTGAGTGCTGTAGAAGCAGGAATAATCTCATTCAATTTTTCACGCTAAATGAAAGGGAGATTAAATA  >  Exported/709975‑710107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: