Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 719,427 C→T 6.4% A254V (GCC→GTC)  mviN → predicted inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported719,4270CT 6.4% 536.2 / 16.1 171A254V (GCC→GTC) mviNpredicted inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (60/100);  new base T (9/2);  total (69/102)
Fisher's exact test for biased strand distribution p-value = 7.68e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

Only 100 of 182 total aligned reads displayed.

AAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACGCCGACCGCTTAATGGAGTTTCCGTCCGG  >  Exported/719357‑719496
                                                                      |                                                                     
aaaCAGATGGGACCGGCGATCGTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGc                                                                       >  1:3973542/1‑71 (MQ=255)
aaaCAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGc                                                                       >  1:4916113/1‑71 (MQ=255)
aaaCAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGc                                                                       >  1:4436830/1‑71 (MQ=255)
   cAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTc                                                                    <  1:3903496/71‑1 (MQ=255)
   cAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTc                                                                    <  1:4490713/71‑1 (MQ=255)
   cAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGAGCTCCTTAATCATCAACACCATTTTTGCCTc                                                                    <  1:1116171/71‑1 (MQ=255)
    aGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTc                                                                    <  1:3834803/70‑1 (MQ=255)
     gATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGt                                                                  <  1:2873358/71‑1 (MQ=255)
     gATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGt                                                                  <  1:3564472/71‑1 (MQ=255)
     gATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGt                                                                  <  1:3733685/71‑1 (MQ=255)
     gATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGt                                                                  <  1:410462/71‑1 (MQ=255)
     gATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGt                                                                  <  1:4532589/71‑1 (MQ=255)
       tGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt                                                                <  1:1534365/71‑1 (MQ=255)
          gACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCtt                                                             <  1:1688303/71‑1 (MQ=255)
          gACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCtt                                                             <  1:4459001/71‑1 (MQ=255)
              ggCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCtt                                                         >  1:2076430/1‑71 (MQ=255)
              ggCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCt                                                          >  1:3945063/1‑70 (MQ=255)
                  aTCCTTGGTGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt                                                                <  1:2545085/60‑1 (MQ=255)
                  aTCCTTGGTGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt                                                                <  1:2380838/60‑1 (MQ=255)
                  aTCCTTGGTGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt                                                                <  1:692156/60‑1 (MQ=255)
                  aTCCTTGGTGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt                                                                <  1:4883503/60‑1 (MQ=255)
                  aTCCTTGGTGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt                                                                <  1:1643530/60‑1 (MQ=255)
                  aTCCTTGGTGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt                                                                <  1:1936416/60‑1 (MQ=255)
                  aTCCTTGGTGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt                                                                <  1:4504577/60‑1 (MQ=255)
                  aTCCTTGGTGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt                                                                <  1:4763206/60‑1 (MQ=255)
                  aTCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCgg                                                     >  1:5041271/1‑71 (MQ=255)
                    ccTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGcc                                                                      >  1:3312206/1‑52 (MQ=255)
                         gCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGgt                                               <  1:2358403/70‑1 (MQ=255)
                          cGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGgtgt                                             <  1:164214/71‑1 (MQ=255)
                          cGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGgtg                                              >  1:2726949/1‑70 (MQ=255)
                           gTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTc                                            <  1:1042355/71‑1 (MQ=255)
                              tctGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt                                                                <  1:4552442/48‑1 (MQ=255)
                              tctGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt                                                                <  1:734033/48‑1 (MQ=255)
                              tctGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt                                                                <  1:1047552/48‑1 (MQ=255)
                              tctGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt                                                                <  1:2031407/48‑1 (MQ=255)
                              tctGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTg                                         >  1:3694539/1‑71 (MQ=255)
                              tctGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTg                                         >  1:385660/1‑71 (MQ=255)
                              tctGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTg                                         >  1:4141895/1‑71 (MQ=255)
                              tctGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTg                                         >  1:221407/1‑71 (MQ=255)
                              tctGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTg                                         >  1:3229410/1‑71 (MQ=255)
                                tgtgAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGt                                                    >  1:4797534/1‑58 (MQ=255)
                                tgtgAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCtt                                          <  1:1831200/68‑1 (MQ=255)
                                tgtgAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCtt                                          <  1:4558029/68‑1 (MQ=255)
                                tgtgAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCtt                                          <  1:3074269/68‑1 (MQ=255)
                                tgtgAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCtt                                          <  1:4296655/68‑1 (MQ=255)
                                tgtgAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCtt                                          <  1:2845540/68‑1 (MQ=255)
                                tgtgAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTg                                         <  1:1326862/69‑1 (MQ=255)
                                 gtgAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGAt                                      >  1:3369420/1‑71 (MQ=255)
                                  tgAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTgg                                        <  1:4022319/68‑1 (MQ=255)
                                  tgAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTgg                                        <  1:3166406/68‑1 (MQ=255)
                                  tgAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg                                     <  1:3787692/71‑1 (MQ=255)
                                    aGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGt                                    <  1:1734764/70‑1 (MQ=255)
                                         gATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTa                                >  1:3907968/1‑69 (MQ=255)
                                         gATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACg                              <  1:2166319/71‑1 (MQ=255)
                                         gATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACg                              >  1:855507/1‑71 (MQ=255)
                                           tctcCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACg                              <  1:881039/69‑1 (MQ=255)
                                           tctcCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACg                              <  1:305567/69‑1 (MQ=255)
                                             tcCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGtt                                                   <  1:756291/46‑1 (MQ=255)
                                             tcCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGtt                                                   <  1:657249/46‑1 (MQ=255)
                                             tcCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGtt                                                   <  1:3349712/46‑1 (MQ=255)
                                             tcCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg                                     <  1:2586224/60‑1 (MQ=255)
                                             tcCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg                                     <  1:1928793/60‑1 (MQ=255)
                                             tcCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg                                     <  1:779196/60‑1 (MQ=255)
                                             tcCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg                                     <  1:2404421/60‑1 (MQ=255)
                                             tcCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg                                     <  1:5137752/60‑1 (MQ=255)
                                                ttAATCATCAACACCATTTTTGTCTCGTTTCTTGCTTCCGGTTCGgtgt                                             <  1:5189936/49‑1 (MQ=255)
                                                  aaTCATCAACACCATTTTTGTCTCGTTTCTTGCTTCCGGTTCGGTGTCt                                           >  1:1991492/1‑49 (MQ=255)
                                                  aaTCATCAACACCATTTTTGTCTCGTTTCTTGCTTCCGGTTCGGTGTCt                                           >  1:1492079/1‑49 (MQ=255)
                                                  aaTCATCAACACCATTTTTGTCTCGTTTCTTGCTTCCGGTTCGGTGTCt                                           >  1:4045730/1‑49 (MQ=255)
                                                  aaTCATCAACACCATTTTTGTCTCGTTTCTTGCTTCCGGTTCGGTGTCt                                           >  1:744396/1‑49 (MQ=255)
                                                  aaTCATCAACACCATTTTTGTCTCGTTTCTTCTTCCGGTTCGGTGTCt                                           >  1:4900159/1‑48 (MQ=38)
                                                   atcatcAACACCATTTTTGCCTCGTTTCTTGCTTCCg                                                      >  1:4046359/1‑37 (MQ=255)
                                                   atcatcAACACCATTTTTGCCTCGTTTCTTGCTTCCg                                                      >  1:1954017/1‑37 (MQ=255)
                                                   atcatcAACACCATTTTTGCCTCGTTTCTTGCTTCCg                                                      >  1:1217372/1‑37 (MQ=255)
                                                   atcatcAACACCATTTTTGCCTCGTTTCTTGCTTCCg                                                      >  1:3609316/1‑37 (MQ=255)
                                                   atcatcAACACCATTTTTGCCTCGTTTCTTGCTTCCg                                                      >  1:3351114/1‑37 (MQ=255)
                                                   atcatcAACACCATTTTTGCCTCGTTTCTTGCTTCCg                                                      >  1:4738125/1‑37 (MQ=255)
                                                   atcatcAACACCATTTTTGCCTCGTTTCTTGCTTCCg                                                      >  1:1340902/1‑37 (MQ=255)
                                                   atcatcAACACCATTTTTGCCTCGTTTCTTGCTTCCg                                                      >  1:3299101/1‑37 (MQ=255)
                                                   atcatcAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACGCCGACCGCtt                    <  1:4628795/71‑1 (MQ=255)
                                                      atcaACTCCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACGCCGACCGCTTaa                  <  1:5051802/70‑1 (MQ=255)
                                                       tcaACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg                                     <  1:567202/50‑1 (MQ=255)
                                                       tcaACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg                                     <  1:2702179/50‑1 (MQ=255)
                                                       tcaACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg                                     <  1:4008680/50‑1 (MQ=255)
                                                        caACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg                                     <  1:5071953/49‑1 (MQ=255)
                                                               aTTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTAtt                                 >  1:1340475/1‑46 (MQ=255)
                                                               aTTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTa                                >  1:2925636/1‑47 (MQ=255)
                                                               aTTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTa                                >  1:4316745/1‑47 (MQ=255)
                                                               aTTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTa                                >  1:5138670/1‑47 (MQ=255)
                                                               aTTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTa                                >  1:421951/1‑47 (MQ=255)
                                                               aTTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACGcc                            <  1:4227375/51‑1 (MQ=255)
                                                               aTTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACGcc                            <  1:2150388/51‑1 (MQ=255)
                                                               aTTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACGcc                            <  1:2239162/51‑1 (MQ=255)
                                                                tttttGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg                                     <  1:1668082/41‑1 (MQ=255)
                                                                   ttGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGa                                       >  1:3846454/1‑36 (MQ=255)
                                                                   ttGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg                                     >  1:4191085/1‑38 (MQ=255)
                                                                   ttGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg                                     >  1:2454574/1‑38 (MQ=255)
                                                                   ttGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg                                     >  1:2142979/1‑38 (MQ=255)
                                                                   ttGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg                                     >  1:4759049/1‑38 (MQ=255)
                                                                      ccTCGTTTCTTGCTTCCGGTTCGGTCTCTTGGATGTATTACGCCGACCGCTTAATGGTGTTTCCGTCCgg  <  1:3281256/70‑1 (MQ=255)
                                                                      |                                                                     
AAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACGCCGACCGCTTAATGGAGTTTCCGTCCGG  >  Exported/719357‑719496

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: