Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 719,427 | C→T | 6.4% | A254V (GCC→GTC) | mviN → | predicted inner membrane protein |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 719,427 | 0 | C | T | 6.4% | 536.2 / 16.1 | 171 | A254V (GCC→GTC) | mviN | predicted inner membrane protein |
Reads supporting (aligned to +/- strand): ref base C (60/100); new base T (9/2); total (69/102) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.68e-03 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01 |
Only 100 of 182 total aligned reads displayed.
AAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACGCCGACCGCTTAATGGAGTTTCCGTCCGG > Exported/719357‑719496 | aaaCAGATGGGACCGGCGATCGTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGc > 1:3973542/1‑71 (MQ=255) aaaCAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGc > 1:4916113/1‑71 (MQ=255) aaaCAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGc > 1:4436830/1‑71 (MQ=255) cAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTc < 1:3903496/71‑1 (MQ=255) cAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTc < 1:4490713/71‑1 (MQ=255) cAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGAGCTCCTTAATCATCAACACCATTTTTGCCTc < 1:1116171/71‑1 (MQ=255) aGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTc < 1:3834803/70‑1 (MQ=255) gATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGt < 1:2873358/71‑1 (MQ=255) gATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGt < 1:3564472/71‑1 (MQ=255) gATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGt < 1:3733685/71‑1 (MQ=255) gATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGt < 1:410462/71‑1 (MQ=255) gATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGt < 1:4532589/71‑1 (MQ=255) tGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt < 1:1534365/71‑1 (MQ=255) gACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCtt < 1:1688303/71‑1 (MQ=255) gACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCtt < 1:4459001/71‑1 (MQ=255) ggCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCtt > 1:2076430/1‑71 (MQ=255) ggCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCt > 1:3945063/1‑70 (MQ=255) aTCCTTGGTGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt < 1:2545085/60‑1 (MQ=255) aTCCTTGGTGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt < 1:2380838/60‑1 (MQ=255) aTCCTTGGTGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt < 1:692156/60‑1 (MQ=255) aTCCTTGGTGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt < 1:4883503/60‑1 (MQ=255) aTCCTTGGTGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt < 1:1643530/60‑1 (MQ=255) aTCCTTGGTGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt < 1:1936416/60‑1 (MQ=255) aTCCTTGGTGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt < 1:4504577/60‑1 (MQ=255) aTCCTTGGTGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt < 1:4763206/60‑1 (MQ=255) aTCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCgg > 1:5041271/1‑71 (MQ=255) ccTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGcc > 1:3312206/1‑52 (MQ=255) gCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGgt < 1:2358403/70‑1 (MQ=255) cGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGgtgt < 1:164214/71‑1 (MQ=255) cGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGgtg > 1:2726949/1‑70 (MQ=255) gTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTc < 1:1042355/71‑1 (MQ=255) tctGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt < 1:4552442/48‑1 (MQ=255) tctGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt < 1:734033/48‑1 (MQ=255) tctGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt < 1:1047552/48‑1 (MQ=255) tctGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGttt < 1:2031407/48‑1 (MQ=255) tctGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTg > 1:3694539/1‑71 (MQ=255) tctGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTg > 1:385660/1‑71 (MQ=255) tctGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTg > 1:4141895/1‑71 (MQ=255) tctGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTg > 1:221407/1‑71 (MQ=255) tctGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTg > 1:3229410/1‑71 (MQ=255) tgtgAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGt > 1:4797534/1‑58 (MQ=255) tgtgAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCtt < 1:1831200/68‑1 (MQ=255) tgtgAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCtt < 1:4558029/68‑1 (MQ=255) tgtgAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCtt < 1:3074269/68‑1 (MQ=255) tgtgAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCtt < 1:4296655/68‑1 (MQ=255) tgtgAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCtt < 1:2845540/68‑1 (MQ=255) tgtgAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTg < 1:1326862/69‑1 (MQ=255) gtgAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGAt > 1:3369420/1‑71 (MQ=255) tgAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTgg < 1:4022319/68‑1 (MQ=255) tgAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTgg < 1:3166406/68‑1 (MQ=255) tgAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg < 1:3787692/71‑1 (MQ=255) aGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGt < 1:1734764/70‑1 (MQ=255) gATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTa > 1:3907968/1‑69 (MQ=255) gATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACg < 1:2166319/71‑1 (MQ=255) gATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACg > 1:855507/1‑71 (MQ=255) tctcCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACg < 1:881039/69‑1 (MQ=255) tctcCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACg < 1:305567/69‑1 (MQ=255) tcCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGtt < 1:756291/46‑1 (MQ=255) tcCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGtt < 1:657249/46‑1 (MQ=255) tcCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGtt < 1:3349712/46‑1 (MQ=255) tcCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg < 1:2586224/60‑1 (MQ=255) tcCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg < 1:1928793/60‑1 (MQ=255) tcCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg < 1:779196/60‑1 (MQ=255) tcCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg < 1:2404421/60‑1 (MQ=255) tcCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg < 1:5137752/60‑1 (MQ=255) ttAATCATCAACACCATTTTTGTCTCGTTTCTTGCTTCCGGTTCGgtgt < 1:5189936/49‑1 (MQ=255) aaTCATCAACACCATTTTTGTCTCGTTTCTTGCTTCCGGTTCGGTGTCt > 1:1991492/1‑49 (MQ=255) aaTCATCAACACCATTTTTGTCTCGTTTCTTGCTTCCGGTTCGGTGTCt > 1:1492079/1‑49 (MQ=255) aaTCATCAACACCATTTTTGTCTCGTTTCTTGCTTCCGGTTCGGTGTCt > 1:4045730/1‑49 (MQ=255) aaTCATCAACACCATTTTTGTCTCGTTTCTTGCTTCCGGTTCGGTGTCt > 1:744396/1‑49 (MQ=255) aaTCATCAACACCATTTTTGTCTCGTTTCTT‑CTTCCGGTTCGGTGTCt > 1:4900159/1‑48 (MQ=38) atcatcAACACCATTTTTGCCTCGTTTCTTGCTTCCg > 1:4046359/1‑37 (MQ=255) atcatcAACACCATTTTTGCCTCGTTTCTTGCTTCCg > 1:1954017/1‑37 (MQ=255) atcatcAACACCATTTTTGCCTCGTTTCTTGCTTCCg > 1:1217372/1‑37 (MQ=255) atcatcAACACCATTTTTGCCTCGTTTCTTGCTTCCg > 1:3609316/1‑37 (MQ=255) atcatcAACACCATTTTTGCCTCGTTTCTTGCTTCCg > 1:3351114/1‑37 (MQ=255) atcatcAACACCATTTTTGCCTCGTTTCTTGCTTCCg > 1:4738125/1‑37 (MQ=255) atcatcAACACCATTTTTGCCTCGTTTCTTGCTTCCg > 1:1340902/1‑37 (MQ=255) atcatcAACACCATTTTTGCCTCGTTTCTTGCTTCCg > 1:3299101/1‑37 (MQ=255) atcatcAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACGCCGACCGCtt < 1:4628795/71‑1 (MQ=255) atcaACTCCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACGCCGACCGCTTaa < 1:5051802/70‑1 (MQ=255) tcaACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg < 1:567202/50‑1 (MQ=255) tcaACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg < 1:2702179/50‑1 (MQ=255) tcaACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg < 1:4008680/50‑1 (MQ=255) caACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg < 1:5071953/49‑1 (MQ=255) aTTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTAtt > 1:1340475/1‑46 (MQ=255) aTTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTa > 1:2925636/1‑47 (MQ=255) aTTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTa > 1:4316745/1‑47 (MQ=255) aTTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTa > 1:5138670/1‑47 (MQ=255) aTTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTa > 1:421951/1‑47 (MQ=255) aTTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACGcc < 1:4227375/51‑1 (MQ=255) aTTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACGcc < 1:2150388/51‑1 (MQ=255) aTTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACGcc < 1:2239162/51‑1 (MQ=255) tttttGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg < 1:1668082/41‑1 (MQ=255) ttGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGa > 1:3846454/1‑36 (MQ=255) ttGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg > 1:4191085/1‑38 (MQ=255) ttGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg > 1:2454574/1‑38 (MQ=255) ttGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg > 1:2142979/1‑38 (MQ=255) ttGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATg > 1:4759049/1‑38 (MQ=255) ccTCGTTTCTTGCTTCCGGTTCGGTCTCTTGGATGTATTACGCCGACCGCTTAATGGTGTTTCCGTCCgg < 1:3281256/70‑1 (MQ=255) | AAACAGATGGGACCGGCGATCCTTGGCGTCTCTGTGAGCCAGATCTCCTTAATCATCAACACCATTTTTGCCTCGTTTCTTGCTTCCGGTTCGGTGTCTTGGATGTATTACGCCGACCGCTTAATGGAGTTTCCGTCCGG > Exported/719357‑719496 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |