| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 775,607 | C→A | 9.4% | D410E (GAC→GAA) | icd → | isocitrate dehydrogenase, specific for NADP+ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 775,607 | 0 | C | A | 9.4% | 242.5 / 7.6 | 74 | D410E (GAC→GAA) | icd | isocitrate dehydrogenase, specific for NADP+ |
| Reads supporting (aligned to +/- strand): ref base C (30/37); new base A (4/3); total (34/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01 | |||||||||||
CCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGG > Exported/775540‑775656 | ccGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcgcg < 1:2662816/71‑1 (MQ=255) cGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGa < 1:1492228/71‑1 (MQ=255) aCTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAAc < 1:2220263/71‑1 (MQ=255) ttCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAAc < 1:200760/69‑1 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaaa > 1:1036730/1‑66 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaaa > 1:2324348/1‑66 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaaa > 1:615486/1‑66 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaaa > 1:4670839/1‑66 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaaa > 1:920139/1‑66 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaaa > 1:4307537/1‑66 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaaa > 1:3838587/1‑66 (MQ=255) cGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTAAGAGTTTGGTGACGCGATCATCGaaaa > 1:702098/1‑66 (MQ=255) gAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt < 1:2675509/70‑1 (MQ=255) gAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt < 1:530199/70‑1 (MQ=255) gAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt < 1:234235/70‑1 (MQ=255) gAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGAGATCATCGAAAACATGt < 1:1174415/70‑1 (MQ=255) aGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt < 1:2489055/69‑1 (MQ=255) atggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGat > 1:2257935/1‑47 (MQ=255) atggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGat > 1:1189648/1‑47 (MQ=255) atggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGat > 1:3074056/1‑47 (MQ=255) ggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGtt > 1:2294506/1‑71 (MQ=255) ggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGtt > 1:1093829/1‑71 (MQ=255) ggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGtt > 1:4302522/1‑71 (MQ=255) ggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGtt > 1:3367555/1‑71 (MQ=255) ggatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGtt > 1:4380685/1‑71 (MQ=255) ggaaggCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGaaaa < 1:3802820/54‑1 (MQ=255) ggaaggCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGaaaa < 1:3796755/54‑1 (MQ=255) gatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGcc < 1:5063702/64‑1 (MQ=255) gatggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGcc < 1:4664684/64‑1 (MQ=255) atggCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGcc < 1:4066564/63‑1 (MQ=255) aaggCGCTAAGCTTCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACa > 1:3348691/3‑54 (MQ=255) aaggCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACa > 1:3927844/3‑54 (MQ=255) aaggCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACa > 1:812079/3‑54 (MQ=255) aaggCGCTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGAAAACa > 1:4743693/3‑54 (MQ=255) gcgcTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTa < 1:3009855/71‑1 (MQ=255) cgcTAAGCTGCTGAAATGTTCAGAGTTTGGTGAAGCGATCATCGaaaa < 1:2074765/48‑1 (MQ=39) cTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAt > 1:4467167/1‑71 (MQ=255) aaCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt > 1:4449925/1‑69 (MQ=255) aaCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt < 1:4193328/69‑1 (MQ=255) aCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCCTCGaaa < 1:3557/41‑1 (MQ=255) aCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCCTCGaaa < 1:668113/41‑1 (MQ=255) aCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaa < 1:5092064/41‑1 (MQ=255) aCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaa < 1:989341/41‑1 (MQ=255) aCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaa < 1:4806127/41‑1 (MQ=255) aCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaa < 1:3963061/41‑1 (MQ=255) aCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaa < 1:1259462/41‑1 (MQ=255) aCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaa < 1:1595957/41‑1 (MQ=255) aCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaa < 1:3043059/41‑1 (MQ=255) aCTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaa < 1:3154665/41‑1 (MQ=255) ctgctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAAc > 1:417380/1‑42 (MQ=255) ctgctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAAc > 1:4547021/1‑42 (MQ=255) ctgctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAAc > 1:4534787/1‑42 (MQ=255) ctgctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt > 1:3731637/1‑67 (MQ=255) ctgctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATttatt < 1:4392002/71‑1 (MQ=255) ctgctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATttatt < 1:1291345/71‑1 (MQ=255) ctgctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATttatt < 1:4315564/71‑1 (MQ=255) ctgctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATttatt < 1:4827184/71‑1 (MQ=255) ctgctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATttatt < 1:2838440/71‑1 (MQ=255) ctgctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATtta > 1:3208381/1‑69 (MQ=255) ctgctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATtta > 1:2457560/1‑69 (MQ=255) gctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATttatta > 1:3424364/1‑70 (MQ=255) gctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATtta > 1:2042733/1‑67 (MQ=255) gctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTaa > 1:104190/1‑71 (MQ=255) ctgAAATGTTCAGAGTTTGGTGACTCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAAc > 1:3621496/1‑71 (MQ=255) ctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt < 1:4735479/43‑1 (MQ=255) ctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt < 1:1512069/43‑1 (MQ=255) ctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt < 1:1314019/43‑1 (MQ=255) ctgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGt < 1:3176522/43‑1 (MQ=255) tgAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt > 1:345075/1‑63 (MQ=255) aaaTGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt < 1:2700924/61‑1 (MQ=255) aaaTGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt < 1:4535902/61‑1 (MQ=255) aaaTGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAAtt < 1:4135935/61‑1 (MQ=255) aaaTGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACgg < 1:4528419/70‑1 (MQ=255) ttCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATtta > 1:1257367/1‑58 (MQ=255) tttGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATttatt < 1:2276199/53‑1 (MQ=255) | CCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGG > Exported/775540‑775656 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |