Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 809,202 | T→C | 5.0% | K275K (AAA→AAG) | ychF ← | predicted GTP‑binding protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 809,202 | 0 | T | C | 5.0% | 235.3 / 2.7 | 79 | K275K (AAA→AAG) | ychF | predicted GTP‑binding protein |
Reads supporting (aligned to +/- strand): ref base T (47/28); new base C (2/2); total (49/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.32e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01 |
CAACCGGAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGCTCCTGCATAAACTCG > Exported/809135‑809268 | cAACCGGAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGtt < 1:2236303/69‑1 (MQ=255) cAACCGGAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTta > 1:1360953/1‑71 (MQ=255) aCCGGAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTtata < 1:2105317/71‑1 (MQ=255) aCCGGAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTtata < 1:844550/71‑1 (MQ=255) aCCGGAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTtata < 1:3482861/71‑1 (MQ=255) aCCGGAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTtata < 1:3904818/71‑1 (MQ=255) aCCGGAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTtata < 1:3996607/71‑1 (MQ=255) ccGGAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATaa > 1:971874/1‑71 (MQ=255) ccGGAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATaa > 1:2873368/1‑71 (MQ=255) ccGGAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATaa > 1:4592320/1‑71 (MQ=255) ccGGAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGCTTATaa < 1:4446611/71‑1 (MQ=255) ccGGAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGCTTATaa < 1:1379591/71‑1 (MQ=255) cGGAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAAc > 1:1478646/1‑71 (MQ=255) tCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACgg < 1:864129/71‑1 (MQ=255) ccATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGa > 1:4435342/1‑71 (MQ=255) ccATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGa > 1:4378961/1‑71 (MQ=255) ccATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGa > 1:3298644/1‑71 (MQ=255) cacTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATcac > 1:1423415/1‑66 (MQ=255) cacTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATcac > 1:2803651/1‑66 (MQ=255) cacTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATcac > 1:2811274/1‑66 (MQ=255) cacTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATcac > 1:505920/1‑66 (MQ=255) cacTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATcac > 1:3933204/1‑66 (MQ=255) cttcttTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACgg < 1:689897/59‑1 (MQ=255) cttcttTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACgg < 1:202342/59‑1 (MQ=255) tcttTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAg > 1:2903481/1‑71 (MQ=255) tcttTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAg > 1:4955142/1‑71 (MQ=255) ccAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAAcc > 1:4222080/1‑41 (MQ=255) ccAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGtt > 1:1109931/1‑59 (MQ=255) ccAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAgg > 1:1877887/1‑63 (MQ=255) ccAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAgg > 1:4202051/1‑63 (MQ=255) ccAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAgg > 1:4267390/1‑63 (MQ=255) ccAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAgg > 1:1888590/1‑63 (MQ=255) ccAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAgg > 1:3212374/1‑63 (MQ=255) ccAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAg > 1:1533818/1‑62 (MQ=255) ccAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGctc > 1:2873018/1‑71 (MQ=255) gCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACAGGGTTCAGGCCCGGctc > 1:3413985/1‑68 (MQ=255) gTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑g < 1:1904617/71‑1 (MQ=255) tGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGctct < 1:2288095/65‑1 (MQ=255) tGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGctct < 1:3970862/65‑1 (MQ=255) tGAAGTAAGTTTGCAGGTTCACCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGctct < 1:4774752/65‑1 (MQ=255) aagtaagtTTGAAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑Gccc < 1:3595357/71‑1 (MQ=255) gtaagtTTGTAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑Gccc > 1:2002028/1‑69 (MQ=255) gtaagtTTGCAGGTTCAGCAGTTTATAACCGGCAc > 1:545982/1‑35 (MQ=255) gtaagtTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑Gccc > 1:4181760/1‑69 (MQ=255) gtaagtTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑Gccc > 1:788391/1‑69 (MQ=255) gtaagtTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑Gccc > 1:2585247/1‑69 (MQ=255) agtTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGTTCTTCCA‑GCCCAAGc < 1:456227/70‑1 (MQ=255) agtTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGc < 1:4633634/70‑1 (MQ=255) agtTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGc < 1:1919890/70‑1 (MQ=255) agtTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGc < 1:3605704/70‑1 (MQ=255) agtCTGCAGGCCCAGCAGCTTGAACGCGGCGCGGATCAGGCGATCCAGGCCCGGCTCGTCCATGCCCatat > 1:1524775/1‑68 (MQ=255) agtCTGCAGGCCCAGCAGCTTGAACGCGGCGCGGATCAGGCGATCCAGGCCCGGCTCGTCCATGCCCatat > 1:825154/1‑68 (MQ=255) gtTTGCAGGTTCAGCAGTTTATAACCGGCACGGATAACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGc < 1:2866296/69‑1 (MQ=255) ttGCAGGTTCAGCAGTTTATAACCGGCACGGATcac > 1:201131/1‑36 (MQ=255) ttGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGCt > 1:3259237/1‑68 (MQ=255) ttGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGCt > 1:449448/1‑68 (MQ=255) ttGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGCt > 1:1844996/1‑68 (MQ=255) gCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGCTCCTg < 1:2460692/70‑1 (MQ=255) gCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGCTCCTg < 1:2729211/70‑1 (MQ=255) gCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGCTCCTg < 1:2947293/70‑1 (MQ=255) gCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGCTCCTg < 1:3721644/70‑1 (MQ=255) ggTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGCTCCTGCATa < 1:4746247/71‑1 (MQ=255) ggTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGCTCCTGCATa < 1:3092165/71‑1 (MQ=255) ggTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGCTCCTGCATa < 1:1721532/71‑1 (MQ=255) ggTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGCTCCTGCATa < 1:1553037/71‑1 (MQ=255) gTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGCTCCTGCATa < 1:1036713/70‑1 (MQ=255) ttCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGCTCCTGCATa < 1:3821927/69‑1 (MQ=255) tCAGCAGTTTATAACCGGCACGGATCACACGGTTc > 1:1173467/1‑35 (MQ=255) tCAGCAGTTTATAACCGGCACGGATCACACGGTTc > 1:3439350/1‑35 (MQ=255) tCAGCAGTTTATAACCGGCACGGATCACACGGTTc > 1:3060226/1‑35 (MQ=255) tCAGCAGTTTATAACCGGCACGGATCACACGGTTc > 1:1176371/1‑35 (MQ=255) tCAGCAGTTTATAACCGGCACGGATCACACGGTTc > 1:1858406/1‑35 (MQ=255) tCAGCAGTTTATAACCGGCACGGATCACACGGTTc > 1:1421044/1‑35 (MQ=255) tCAGCAGTTTATAACCGGCACGGATCACACGGTTc > 1:2491692/1‑35 (MQ=255) tCAGCAGTTTATAACCGGCACGGATCACACGGTTc > 1:1324625/1‑35 (MQ=255) tCAGCAGTTTATAACCGGCACGGATCACACGGTTc > 1:4179861/1‑35 (MQ=255) tCAGCAGTTTATAACCGGCACGGATCACACGGTTc > 1:644447/1‑35 (MQ=255) gcagTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGCTCCTGCATAAACTCg > 1:4116666/1‑71 (MQ=255) gcagTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGCTCCTGCATAAACTCg > 1:2003882/1‑71 (MQ=255) gcagTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGCTCCTGCATAAACTCg > 1:5051012/1‑71 (MQ=255) tttATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGc < 1:1276752/52‑1 (MQ=255) tttATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGc < 1:188544/52‑1 (MQ=255) tttATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGc < 1:3651168/52‑1 (MQ=255) tttATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGc < 1:3820867/52‑1 (MQ=255) | CAACCGGAATGGTCCATGCACGCACTTCTTTCACCCCAGCGGTGAAGTAAGTTTGCAGGTTCAGCAGTTTATAACCGGCACGGATCACACGGTTCAGGCCCGGCTCTTCCA‑GCCCAAGCTCCTGCATAAACTCG > Exported/809135‑809268 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |