| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,314,255 | T→C | 9.1% | H170H (CAT→CAC) | hisB → | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,314,255 | 0 | T | C | 9.1% | 479.0 / 28.0 | 165 | H170H (CAT→CAC) | hisB | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Reads supporting (aligned to +/- strand): ref base T (68/81); new base C (9/6); total (78/87) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.17e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
Only 100 of 165 total aligned reads displayed.
ACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGC > Exported/1314188‑1314321 | cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAg > 1:4897091/1‑71 (MQ=255) cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAg > 1:1533311/1‑71 (MQ=255) gcgAAACCCTGAACTGGCCAATGATTGGCTAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCg > 1:1424555/1‑70 (MQ=255) gcgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGt < 1:4373050/71‑1 (MQ=255) gcgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGt < 1:2629900/71‑1 (MQ=255) cccTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATAcc < 1:832689/71‑1 (MQ=255) cccTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATAcc < 1:3515318/71‑1 (MQ=255) ccTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGt < 1:3337159/64‑1 (MQ=255) ccTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGt < 1:5039202/64‑1 (MQ=255) ccTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGt < 1:1899191/64‑1 (MQ=255) ccTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGt < 1:3089281/64‑1 (MQ=255) ccTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGt < 1:810835/64‑1 (MQ=255) cTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTa > 1:4885427/1‑57 (MQ=255) cTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTa > 1:4323976/1‑57 (MQ=255) cTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTa > 1:267304/1‑57 (MQ=255) gAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCa > 1:3775269/1‑68 (MQ=255) aaCTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACTCTCACGTAGTGCGTAATACCa < 1:1805889/67‑1 (MQ=255) aaCTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCa < 1:2592339/67‑1 (MQ=255) aaCTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCa < 1:314429/67‑1 (MQ=255) aCTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCa < 1:3555971/66‑1 (MQ=255) cTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAgaga < 1:1984503/71‑1 (MQ=255) cTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCaaa > 1:17229/1‑67 (MQ=255) cTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCaaa > 1:2486804/1‑67 (MQ=255) cTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCaaa > 1:4229983/1‑67 (MQ=255) cTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCaaa > 1:2766331/1‑67 (MQ=255) tGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGt < 1:2463056/50‑1 (MQ=255) cAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACg > 1:5028104/1‑68 (MQ=255) cAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACg > 1:4603086/1‑68 (MQ=255) cAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACg > 1:1793442/1‑68 (MQ=255) cAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACg > 1:887210/1‑68 (MQ=255) cAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACg > 1:5008817/1‑68 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:4574255/1‑70 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:1585272/1‑70 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:253287/70‑1 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:4418471/70‑1 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:1008812/70‑1 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:1272825/70‑1 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:4719074/70‑1 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:1365159/70‑1 (MQ=255) tGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACg > 1:4176480/1‑71 (MQ=255) ggCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAag > 1:1244201/1‑55 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAaga < 1:4117663/52‑1 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAaga < 1:5125765/52‑1 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAaga < 1:1766171/52‑1 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:4266122/1‑64 (MQ=255) aGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:1851175/1‑64 (MQ=255) gCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAaga < 1:1257516/51‑1 (MQ=255) gCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg < 1:1656102/71‑1 (MQ=255) gCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg < 1:2961535/71‑1 (MQ=255) gCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg < 1:2789752/71‑1 (MQ=255) cAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg < 1:2149265/70‑1 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:320948/1‑71 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:1790809/1‑71 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:4959977/1‑71 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:2889080/1‑71 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:2113859/1‑71 (MQ=255) cACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:4773626/1‑71 (MQ=255) aCCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCt > 1:3225564/1‑71 (MQ=255) aCCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCt > 1:459884/1‑71 (MQ=255) aCCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCt > 1:443558/1‑71 (MQ=255) aCCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCt > 1:4341802/1‑71 (MQ=255) cAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTgg < 1:3747129/71‑1 (MQ=255) cAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTgg < 1:686964/71‑1 (MQ=255) cAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTg > 1:1914775/1‑70 (MQ=255) gACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc < 1:824663/66‑1 (MQ=255) gACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc < 1:2743391/66‑1 (MQ=255) gACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc < 1:1608998/66‑1 (MQ=255) gACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc < 1:3411827/66‑1 (MQ=255) tGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTg < 1:4081040/71‑1 (MQ=255) tGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTg < 1:2177690/71‑1 (MQ=255) tGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTg < 1:1863973/71‑1 (MQ=255) aCCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGa < 1:2819559/70‑1 (MQ=255) aCCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGa < 1:3264663/70‑1 (MQ=255) ccGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATTGTGAAg < 1:5349/71‑1 (MQ=255) ccGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGa < 1:5030434/69‑1 (MQ=255) ccGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAg < 1:4659851/71‑1 (MQ=255) ccGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAg < 1:1026924/71‑1 (MQ=255) ccGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAg < 1:1385081/71‑1 (MQ=255) ccGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAg < 1:2206856/71‑1 (MQ=255) ccGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAg < 1:2573922/71‑1 (MQ=255) gTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:4015589/1‑57 (MQ=255) gTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:2108208/1‑57 (MQ=255) gTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:2327660/1‑57 (MQ=255) gTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:2630871/1‑57 (MQ=255) gTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:3267355/1‑57 (MQ=255) gTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:3927542/1‑57 (MQ=255) gTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:4673784/1‑57 (MQ=255) gTTACGCTCATGTAGTGCGTAATACCAAAGAGAAGCACATTGa > 1:2574003/1‑43 (MQ=255) attACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc > 1:3934196/2‑57 (MQ=255) ttACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGc < 1:4936718/56‑1 (MQ=255) ttACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCg < 1:1169022/63‑1 (MQ=255) ttACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATCGACGTTCAGGTGTGGCTGGATCg < 1:724097/63‑1 (MQ=255) cGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTgg > 1:512816/1‑69 (MQ=255) cTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGgcagc < 1:4458385/71‑1 (MQ=255) cTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGgcagc < 1:1336521/71‑1 (MQ=255) cTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGgcagc < 1:1157994/71‑1 (MQ=255) tCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGgcagc > 1:3392973/1‑70 (MQ=255) tCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGgcagc > 1:3668002/1‑70 (MQ=255) aTGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGgcagc > 1:3236493/1‑68 (MQ=255) aTGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGgcagc > 1:3582306/1‑68 (MQ=255) | ACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCATGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGGTGTGGCTGGATCGTGAAGGTGGCAGC > Exported/1314188‑1314321 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |