| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,614,453 | G→C | 7.2% | intergenic (‑78/+139) | eutH ← / ← eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,614,453 | 0 | G | C | 7.2% | 222.4 / 6.1 | 69 | intergenic (‑78/+139) | eutH/eutG | predicted inner membrane protein/predicted alcohol dehydrogenase in ethanolamine utilization |
| Reads supporting (aligned to +/- strand): ref base G (28/36); new base C (3/2); total (31/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
CTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCG > Exported/1614384‑1614510 | cTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGt < 1:3836804/71‑1 (MQ=255) ccTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAgg > 1:1153290/1‑71 (MQ=255) ccTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAgg < 1:4642306/71‑1 (MQ=255) ccTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAgg < 1:1750684/71‑1 (MQ=255) ccTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAgg < 1:1656801/71‑1 (MQ=255) cTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAgg < 1:1519650/70‑1 (MQ=255) cTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGc > 1:3382255/1‑71 (MQ=255) gTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTg > 1:188669/1‑71 (MQ=255) gttgttATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGAt < 1:2099286/70‑1 (MQ=255) ttatttatTGGCGGATGCGTCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGAc < 1:3870269/71‑1 (MQ=255) tatttattGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGAc < 1:4947492/70‑1 (MQ=255) acttattGCCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAgg > 1:1281807/3‑58 (MQ=255) tattGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTa > 1:4052150/1‑53 (MQ=255) tattGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTa > 1:1965081/1‑53 (MQ=255) tattGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTa > 1:689407/1‑53 (MQ=255) aTGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTa < 1:196303/44‑1 (MQ=38) aTGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTa < 1:4181370/44‑1 (MQ=38) aTGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTa < 1:5195902/44‑1 (MQ=38) aTGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTa < 1:1438570/44‑1 (MQ=38) aTGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTa < 1:2418364/44‑1 (MQ=38) aTGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTa < 1:3338214/44‑1 (MQ=38) aTGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTa < 1:262155/44‑1 (MQ=38) tGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTa < 1:3860505/43‑1 (MQ=38) gcggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAgg > 1:84550/1‑44 (MQ=38) gcggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAgg > 1:696325/1‑44 (MQ=38) gcggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAgg > 1:2599301/1‑44 (MQ=38) gcggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAgg > 1:2478169/1‑44 (MQ=38) gcggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCAt > 1:413232/1‑71 (MQ=39) cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATc > 1:4821866/1‑71 (MQ=39) cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATc > 1:5023755/1‑71 (MQ=39) cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATc > 1:1548697/1‑71 (MQ=39) cggcgTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATc > 1:503915/1‑71 (MQ=39) gTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGc < 1:1030584/71‑1 (MQ=39) gTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGc < 1:495362/71‑1 (MQ=39) aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:270339/1‑71 (MQ=38) aaCGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAAt > 1:3753546/1‑71 (MQ=38) gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc < 1:4344863/71‑1 (MQ=255) gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc < 1:400448/71‑1 (MQ=255) gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc < 1:3494483/71‑1 (MQ=255) gCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGc < 1:638910/71‑1 (MQ=255) cTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGc < 1:645112/69‑1 (MQ=11) cTTATCCGCCCTACATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGc < 1:284218/69‑1 (MQ=11) cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc < 1:3337926/71‑1 (MQ=255) cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc < 1:3801480/71‑1 (MQ=255) cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc > 1:677866/1‑71 (MQ=255) cTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCAc < 1:1768806/71‑1 (MQ=255) tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:1698984/46‑1 (MQ=38) tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:4874531/46‑1 (MQ=38) tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:4770734/46‑1 (MQ=38) tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:1305743/46‑1 (MQ=38) tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:2228795/46‑1 (MQ=38) tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:2256118/46‑1 (MQ=38) tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:3583129/46‑1 (MQ=38) tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:2677118/46‑1 (MQ=38) tATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCa < 1:24955/46‑1 (MQ=38) aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcgc > 1:1454422/1‑71 (MQ=255) aTCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACcg > 1:4521302/1‑70 (MQ=255) tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAg > 1:2626482/1‑46 (MQ=38) tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAg > 1:2332232/1‑46 (MQ=38) tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAg > 1:2363660/1‑46 (MQ=38) tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAg > 1:3216677/1‑46 (MQ=38) tCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAg > 1:2876117/1‑46 (MQ=38) accGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCaa > 1:5169620/2‑45 (MQ=38) cGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCgcgc < 1:1875160/70‑1 (MQ=255) aCATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGc > 1:3971104/1‑71 (MQ=255) cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg > 1:3481713/1‑71 (MQ=14) cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg > 1:711761/1‑71 (MQ=14) cATGTGCAATCCTCCTGGCCGGATAAAACGCGGCAAGCGTTGCATCCGGCAACTGCACCGCGCCACTGGCg > 1:2517284/1‑71 (MQ=14) cATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGc < 1:2539557/70‑1 (MQ=255) | CTCCCTGTTAGTTGTTATTTATTGGCGGATGCGGCGTAAACGCCTTATCCGCCCTACATGTGCAATCCCGTAGGCTGGATAAGACGCGGCAAGCGTCGCATCCGGCAATTGCACCGCGCCACTGGCG > Exported/1614384‑1614510 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |