Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC AP009048 2,176,983 Δ1,199 bp 100% [gatA][gatA] [gatA], insH, [gatA]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ AP009048 2176983–2178180 2178181 2–1199 60 [0] [0] 60 [gatA]–[gatA] [gatA],insH,[gatA]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP009048 = 21769820 (0.000)41 (0.910) 30/92 NT 100% coding (71/321 nt) gatA galactitol‑specific enzyme IIA component of PTS
?AP009048 2178182 = 0 (0.000)coding (202/315 nt) gatA galactitol‑specific enzyme IIA component of PTS

CGTCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AP009048/2176908‑2176982
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  AP009048/2178182‑2178250
                                                                                                                                                
CGTCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGAC                                                                           <  1:1238590/71‑1
    GTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG                                                                       >  1:209094/1‑71
       ATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCAT                                                                    >  1:3114766/1‑71
          ATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGA                                                                  <  1:2919236/70‑1
          ATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTA                                                                        >  1:2777690/1‑64
               CCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATG                                                                   <  1:2607266/64‑1
                CGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCAT                                                                    <  1:3625709/62‑1
                 GCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTA                                                                        >  1:2197429/1‑57
                    TGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCAC                                                        >  1:4104422/1‑70
                      CTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAAT                                                     <  1:430770/71‑1
                       TGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATG                                                    >  1:276621/1‑71
                       TGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATG                                                    >  1:3794194/1‑71
                       TGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                      <  1:1691935/69‑1
                       TGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAA                                                                 <  1:4544186/58‑1
                         AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCG                                                  >  1:4110102/1‑71
                         AAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATG                                                    >  1:152944/1‑69
                          AAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGC                                                   >  1:103118/1‑69
                           AATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAAT                                                                >  1:778443/1‑55
                            ATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTA                                               >  1:1085218/1‑71
                            ATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTA                                               <  1:161534/71‑1
                            ATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCAT                                                                    <  1:2766416/50‑1
                             TGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTAT                                              >  1:2769840/1‑71
                             TGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCT                                                           <  1:654153/58‑1
                              GAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATG                                                                   >  1:2707449/1‑49
                               AACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATT                                             <  1:3261803/70‑1
                                 CTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCA                                          <  1:324314/71‑1
                                  TTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTT                                                                         <  1:2346436/39‑1
                                   TTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                         >  1:3817998/1‑70
                                   TTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAA                                         >  1:1537627/1‑70
                                     ATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATA                                       <  1:2809940/70‑1
                                      TTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCAT                                                                    >  1:3508787/1‑40
                                       TTGTTGGCCTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCG                                    >  1:2345694/1‑71
                                               CTTAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAAT                                                     <  1:3330532/46‑1
                                                TTAACAGATAAATGGCTGACTACTTAGCATGAATCGCCTCA                                                         <  1:1953640/41‑1
                                                 TAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTAT                                              >  1:4434963/1‑51
                                                 TAACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                      >  1:3162895/1‑43
                                                  AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAG                         >  1:3987016/1‑71
                                                  AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAA                                                      >  1:3734031/1‑42
                                                  AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACA                                                       >  1:2792897/1‑41
                                                  AACAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACA                                                       >  1:1703535/1‑41
                                                    CAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCA                       <  1:3256738/71‑1
                                                    CAGATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCA                       <  1:3124049/71‑1
                                                      GATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATT                     <  1:1599759/71‑1
                                                      GATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAAT                                        >  1:633983/1‑52
                                                       ATAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTA                    >  1:2932865/1‑71
                                                        TAAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGG                                                 <  1:4252896/41‑1
                                                         AAATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATC                  >  1:4267205/1‑71
                                                           ATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCC                >  1:1703846/1‑71
                                                           ATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCC                >  1:4439266/1‑71
                                                           ATGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCA                       >  1:1439412/1‑64
                                                            TGGCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCG               <  1:3321278/71‑1
                                                              GCTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGT             <  1:860691/71‑1
                                                               CTGACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTA            <  1:154718/71‑1
                                                                 GACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGt              <  1:4465351/67‑2
                                                                 GACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGG              <  1:2697/67‑1
                                                                  ACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGG         <  1:631749/71‑1
                                                                  ACGACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGG              <  1:1774421/66‑1
                                                                     ACTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGCTAGGGAAT      <  1:2362707/71‑1
                                                                      CTTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAAT      >  1:713415/1‑70
                                                                       TTAGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATC                  >  1:1393173/1‑57
                                                                         AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  1:278607/1‑71
                                                                         AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  1:2039808/1‑71
                                                                         AGCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCT   <  1:1486443/70‑1
                                                                          GCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCT   <  1:1453920/69‑1
                                                                                                                                                
CGTCGTTATCATCATCCGCTTGCTGAAAATGAACTTTATTTGTTGGCCTTAACAGATAAATGGCTGACGACTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AP009048/2176908‑2176982
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cttagCATGAATCGCCTCACAATGCGGTATTGCAATAGCGTGCTGCTCAAGCATTATCCCGGTAGGGAATTCTG  >  AP009048/2178182‑2178250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.