New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP009048 = 401005566 (1.320)3 (0.070) 3/94 NT 4.7% coding (243/1068 nt) yhiI predicted HlyD family secretion protein
?AP009048 = 4010069 62 (1.350)coding (257/1068 nt) yhiI predicted HlyD family secretion protein
Rejected: Frequency below/above cutoff threshold.

TATCGACACCATTCTGGTGAAAGAAGGCAAGTTTGTTCGCGAAGGTGAAGTGCTGGCGAAGATGGATACTCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AP009048/4009983‑4010055
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcgcTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTT  <  AP009048/4010069‑4010002
                                                                                                                                             
TATCGACACCATTCTGGTGAAAGAAGGCAAGTTTGTTCGCGAAGGTGAAGTGCTGGCGAAGATGGATACTC                                                                        <  1:1939338/71‑1
TATCGACACCATTCTGGTGAAAGAAGGCAAGTTTGTTCGCGAAGGTGAAGTGCTGGCGAAGATGGATACTC                                                                        >  1:3451081/1‑71
 ATCGACACCATTCTGGTGAAAGAAGGCAAGTTTGTTCGCGAAGGTGAAGTGCTGGCGAAGATGGATACTCG                                                                       >  1:2990330/1‑71
 ATCGACACCATTCTGGTGAAAGAAGGCAAGTTTGTTCGCGAAGGTGAAGTGCTGGCGAAGATGGATACTCG                                                                       >  1:4016055/1‑71
   CGACACCATTCTGGTGAAAGAAGGCAAGTTTGTTCGCGAAGGTGAAGTGCTGGCGAAGATGGATACT                                                                         >  1:1120194/1‑67
   CGACACCATTCTGGTGAAAGAAGGCAAGTTTGTTCGCGAAGGTGAAGTGCTGGCGAAGATGGATACT                                                                         >  1:4112326/1‑67
     ACACCATTCTGGTGAAAGAAGGCAAGTTTGTTCGCGAAGGTGAAGTGCTGGCGAAGATGGATACTC                                                                        <  1:2529860/66‑1
     ACACCATTCTGGTGAAAGAAGGCAAGTTTGTTCGCGAAGGTGAAGTGCTGGCGAAGATGGATACTC                                                                        <  1:4117954/66‑1
             CTGGTGAAAGAAGGCAAGTTTGTTCGCGAAGGTGAAGTGCTGGCGAAGATGGATACTCGC                                                                      >  1:322515/1‑60
                 TGAAAGAAGGCAAGTTTGTTCGCGAAGGTGAAGTGCTGGCGAAGATGGATACTCGC                                                                      <  1:922068/56‑1
                     AGAAGGCAAGTTTGTTCGCGAAGGTGAAGTGCTGGCGAAGATGGATACTCGCTGTTCCTGCAACACGCGAG                                                   <  1:2879712/71‑1
                      GAAGGCAAGTTTGTTCGCGAAGGTGAAGTGCTGGCGAAGATGGATACTCGCTGTTCCTGCAACACGCGAGT                                                  <  1:3956914/71‑1
                           CAAGTTTGTTCGCGAAGGTGAAGTGCTGGCGAAGATGGATACTCGCTGTTCCTGCAACACGCGA                                                    >  1:934834/1‑64
                                    TCGCGAAGGTGAAGTGCTGGCGAAGATGGATACTC                                                                        <  1:4025482/35‑1
                                                                     TCGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTT   >  1:209849/1‑71
                                                                      CGCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCAC                           >  1:1300649/1‑46
                                                                       GCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTT  <  1:633202/70‑1
                                                                       GCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTT  <  1:1988291/70‑1
                                                                       GCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTT  <  1:964180/70‑1
                                                                       GCTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTC                       >  1:3440502/1‑49
                                                                                                                                             
TATCGACACCATTCTGGTGAAAGAAGGCAAGTTTGTTCGCGAAGGTGAAGTGCTGGCGAAGATGGATACTCGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AP009048/4009983‑4010055
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tcgcTGTTCCTGCAACACGCGAGTATCCATCTTCGCCAGCACTTCACCTTCGCGAACAAACTTGCCTTCTTT  <  AP009048/4010069‑4010002

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.