New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP009048 4458808 =84 (1.680)3 (0.070) 3/94 NT 4.3% coding (546/996 nt) yjfF predicted sugar transporter subunit
?AP009048 4458830 = 56 (1.220)coding (568/996 nt) yjfF predicted sugar transporter subunit
Rejected: Frequency below/above cutoff threshold.

AGTGGAAATCCCCATCAGATTCGCCGACGTTGCGTTGCCGCCAATGGCGTATACCTGATTACCAAAACGGGTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AP009048/4458880‑4458808
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggtaTACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCA  >  AP009048/4458830‑4458899
                                                                                                                                               
AGTGGAAATCCCCATCAGATTCGCCGACGTTGCGTTGCCGCCAATGGCGTATACCTGATTACCAAAACGGG                                                                          <  1:3777125/71‑1
  TGGAAATCCCCATCAGATTCGCCGACGTTGCGTTGCCGCCAATGGCGTATACCTGATTACCAAAACGGGTA                                                                        <  1:2133646/71‑1
  TGGAAATCCCCATCAGATTCGCCGACGTTGCGTTGCCGCCAATGGCGTATACCTGATTACCAAAACGGGT                                                                         <  1:2286522/70‑1
    GAAATCCCCATCAGATTCGCCGACGTTGCGTTGCCGCCAATGGCGTATACCTGATTACCAAAACGGGT                                                                         <  1:3509691/68‑1
            CATCAGATTCGCCGACGTTGCGTTGCCGCCAATGGCGTATACCTGATTACCAAAACGGGTA                                                                        <  1:419843/61‑1
              TCAGATTCGCCGACGTTGCGTTGCCGCCAATGTCGTATACCTGATTACCAAAACGG                                                                           >  1:521515/1‑56
                          ACGTTGCGTTGCCGCCAATGGCGTATACCTGATTACCAAAACGGGTA                                                                        >  1:3218059/1‑47
                                                   TACCTGATTACCAAAACGGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCA                       >  1:4006645/1‑71
                                                      CTGATTACCAAAACGGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTT                          >  1:4290610/1‑65
                                                        GATTACCAAAACGGGTATACGCCATTGGCGGCAACGCAACGTCGGCGAA                                        >  1:3199153/1‑49
                                                                      tTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTA        <  1:4587571/66‑1
                                                                      GTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATTGGGAT                            <  1:3787023/47‑1
                                                                      GTATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGAT                            <  1:3673391/47‑1
                                                                       TATACGCCATTGGCGGCAACGCAACGTCGGCGATTCTGA                                   <  1:2600417/39‑1
                                                                        ATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCA  >  1:3772352/1‑71
                                                                        ATACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCA  >  1:4490725/1‑71
                                                                                                                                               
AGTGGAAATCCCCATCAGATTCGCCGACGTTGCGTTGCCGCCAATGGCGTATACCTGATTACCAAAACGGGTA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AP009048/4458880‑4458808
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggtaTACGCCATTGGCGGCAACGCAACGTCGGCGAATCTGATGGGGATTTCCACTCGCAGCACCACTATTCGCA  >  AP009048/4458830‑4458899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.