| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,684,927 | A→G | 100% | pseudogene (132/582 nt) pseudogene (1195/1645 nt) |
ilvG ← ilvG ← |
ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,684,927 | 0 | A | G | 100.0% | 112.7 / NA | 34 | pseudogene (132/582 nt) pseudogene (1195/1645 nt) | ilvG ilvG | ECK3760:JW3741:b3768; acetolactate synthase II, large subunit, C‑ter fragment ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (21/13); total (21/13) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTTCGGTCGCGCGACTTGTGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTACCTAAACCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGATGTGCTGCGCAGCCCACATCTGGTGCT > AP009048/3684867‑3684994 | gTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTg > 1:4053355/1‑67 (MQ=37)gTTTGGTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAg < 1:297134/70‑1 (MQ=38) tttggTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGt < 1:2659561/68‑1 (MQ=38) tggTCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGtga > 1:1917852/2‑71 (MQ=38) tCGCGCGACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGtgatga < 1:2599238/71‑1 (MQ=38) cgcgcgACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGtgatga > 1:813893/1‑70 (MQ=38) gcgcgACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGtg < 1:4197841/65‑1 (MQ=38) gcgACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAg > 1:1898285/1‑60 (MQ=37) gACTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAAtttt > 1:4122375/1‑71 (MQ=38) aCTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTgg < 1:4018472/55‑1 (MQ=37) cTTGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAAt > 1:1744106/1‑66 (MQ=38) ttGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCg > 1:230175/1‑71 (MQ=38) ttGCGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGAGCCTAAGCCGCTGGAGGtg > 1:3040201/1‑58 (MQ=37) gcgcgcCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCggg > 1:256474/3‑71 (MQ=38) gcgcgcCAACCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCg < 1:2635831/67‑1 (MQ=38) aCCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGtgtg > 1:928997/1‑71 (MQ=255) aCCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGtgtg > 1:1859215/1‑71 (MQ=255) aCCGCCGCCGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGtgtg > 1:1747768/1‑71 (MQ=255) aCCGCCGACGGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGtgtg > 1:2905280/1‑71 (MQ=39) ccgGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAgtgt < 1:2191783/63‑1 (MQ=255) ccgGTAAACCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGAtg > 1:3952175/1‑71 (MQ=255) aaaCCAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGATGtgct > 1:2986409/1‑70 (MQ=255) ccAAAACCCATGGTGCCTAAGCCGCTGGAGGtgatg < 1:3952750/36‑1 (MQ=37) cAAAACCCATGGTGCCTAAGCCGCTGGAGGTGATGAAAtttt < 1:4086665/42‑1 (MQ=38) aaaCCCATGGTGCCTAAGCCGCTGGAGGTGATGaaa > 1:1320421/1‑36 (MQ=37) tGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAgtg < 1:4164422/44‑1 (MQ=38) tGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGATGTGCTGCGCAGCCCACATc > 1:388540/1‑71 (MQ=255) tGGTGCCTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGATGTGCTGCGCAGCCCACATc > 1:2090274/1‑71 (MQ=255) gtgccTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGATGTGCTGCGCAGCCCACATCTg < 1:3506474/68‑1 (MQ=255) gtgccTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGATGTGCTGCGCAGCCCACATCTg < 1:27874/68‑1 (MQ=255) gtgccTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGATGTGCTGCGCAGCCCACATCTg > 1:1724909/4‑71 (MQ=255) cTAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGATGTGCTGCGCAGCCCACATCTGGtgc > 1:3313097/1‑71 (MQ=255) tAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAgtg < 1:3067628/37‑1 (MQ=255) tAAGCCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGATGTGCTGCGCAGCCCACATCTGGtgct > 1:3061898/1‑71 (MQ=255) | GTTCGGTCGCGCGACTTGTGCGCCAACCGCCGCCGGTAAACCAAAACCCATGGTACCTAAACCGCTGGAGGTGATGAAATTTTCCGGGCGAGTGTGGGCGATGTGCTGCGCAGCCCACATCTGGTGCT > AP009048/3684867‑3684994 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |