| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,685,312 | G→A | 100% | pseudogene (810/1645 nt) pseudogene (810/984 nt) |
ilvG ← ilvG ← |
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,685,312 | 0 | G | A | 100.0% | 214.1 / NA | 73 | pseudogene (810/1645 nt) pseudogene (810/984 nt) | ilvG ilvG | ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (34/39); total (34/39) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ATGGATAACACTGGCGTGTGGCGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGCAGGTCACACTCCTGCACCGCGAAGTTTGCCGCTTTGGTGCCGTGCATCCCC > AP009048/3685246‑3685378 | atGGATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCa > 1:2655023/1‑71 (MQ=255) gATAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACg < 1:3716314/70‑1 (MQ=255) tAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGc > 1:2465722/1‑70 (MQ=255) tAACACTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCg < 1:2348951/71‑1 (MQ=255) acacTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGAt > 1:974088/1‑71 (MQ=255) cacTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGAt > 1:2482841/1‑70 (MQ=255) acTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGAt > 1:724582/1‑69 (MQ=255) acTGGCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATca < 1:386959/71‑1 (MQ=255) gCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagc < 1:626850/69‑1 (MQ=255) gCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagc < 1:2655436/69‑1 (MQ=255) gCGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagc < 1:3915749/69‑1 (MQ=255) cGTGTGGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagcaag < 1:1513324/71‑3 (MQ=39) tgtgGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTc < 1:2247117/71‑1 (MQ=39) tgGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTcgc < 1:2554906/71‑3 (MQ=38) tgGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTcg > 1:4183968/1‑69 (MQ=38) tgGTGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTcg > 1:970183/1‑69 (MQ=38) cgAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTcc > 1:214810/1‑71 (MQ=39) gAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagaagtcg < 1:1539181/63‑4 (MQ=38) aaGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTcgca < 1:2722404/65‑1 (MQ=38) aGGTGTTCAGTTTGCCGGTCACCCGGTCATCAATACGTGCACCCACGGCGATca < 1:3094890/54‑1 (MQ=38) aGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTg > 1:2391428/1‑70 (MQ=38) aGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGc < 1:598486/71‑1 (MQ=39) ggTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagca < 1:377507/56‑1 (MQ=255) ggTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagca < 1:2383922/56‑1 (MQ=255) gtgtTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagc > 1:1852833/1‑54 (MQ=255) gtgtTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTcc < 1:2957345/66‑1 (MQ=38) gtgtTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCAc > 1:2848726/1‑71 (MQ=39) ttCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGc < 1:62984/44‑1 (MQ=255) ttCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGt < 1:2084762/55‑1 (MQ=38) ttCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcgc > 1:515027/1‑71 (MQ=39) ttCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcg > 1:2244730/1‑70 (MQ=38) cAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTcgca > 1:4399263/1‑57 (MQ=37) cAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcgcg < 1:1736182/70‑1 (MQ=38) cAGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCCATCAGCAAGTCGCACTCCTGCACcgcg > 1:1199010/1‑70 (MQ=38) aGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTcc < 1:1919437/60‑1 (MQ=38) aGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcgcg < 1:4280576/69‑1 (MQ=38) aGTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGaa < 1:4508883/71‑1 (MQ=39) gTTTGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGc > 1:2438748/1‑62 (MQ=38) ttGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTcg > 1:1778197/1‑50 (MQ=37) ttGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCa > 1:2215571/1‑61 (MQ=38) tGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCa > 1:4160191/1‑60 (MQ=38) tGCCGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGtt < 1:3910441/70‑1 (MQ=255) ccGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttcg < 1:3346048/70‑3 (MQ=255) cGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATcagca < 1:2073388/42‑1 (MQ=255) cGGTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGt < 1:4566081/66‑1 (MQ=39) gTCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTcgca > 1:454808/1‑47 (MQ=25) tCACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCt < 1:1062498/71‑1 (MQ=255) cACCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCg > 1:3216843/1‑68 (MQ=38) aCCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTcc < 1:3575046/48‑1 (MQ=37) aCCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCtt < 1:3692335/70‑1 (MQ=255) aCCCGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCtt < 1:3455647/70‑1 (MQ=255) cccGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTg < 1:1469245/71‑1 (MQ=255) cccGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTg < 1:3564162/71‑1 (MQ=255) ccGGTCATCAAAGCGTGCACCCACGGCGATcagca < 1:1540825/35‑1 (MQ=37) ccGGTCATCAAAACGTGCACCCACGGCGTTCAGCAAGTCGCACTCCTGCa > 1:109213/1‑50 (MQ=25) ccGGTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTgg > 1:1223648/1‑71 (MQ=255) gTCATCAAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGc < 1:748340/71‑1 (MQ=255) tcatcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACcg < 1:3363712/49‑1 (MQ=38) catcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGcca > 1:4463047/1‑70 (MQ=255) catcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGcca > 1:3621103/1‑70 (MQ=255) catcaAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGcca > 1:1825185/1‑70 (MQ=255) caAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGtt > 1:747926/1‑52 (MQ=255) caAAACGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATg > 1:3801356/1‑70 (MQ=255) aaaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCa > 1:3235604/1‑71 (MQ=255) aaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttcg < 1:9835/52‑3 (MQ=37) aaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGttcg < 1:214782/52‑3 (MQ=37) aaaCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCa > 1:70165/1‑70 (MQ=255) aCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc > 1:2967194/1‑54 (MQ=37) aCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTcgccgc > 1:2049150/1‑54 (MQ=37) aCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAttc < 1:2749793/71‑3 (MQ=255) aCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAttc > 1:2820882/1‑69 (MQ=255) aCGTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCAttc > 1:4431223/1‑69 (MQ=255) gTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtccc < 1:4446540/71‑1 (MQ=255) | ATGGATAACACTGGCGTGTGGCGCGAAGGTGTTCAGTTTGCCGGTCACCCGGTCATCAAAACGTGCGCCCACGGCGATCAGCAGGTCACACTCCTGCACCGCGAAGTTTGCCGCTTTGGTGCCGTGCATCCCC > AP009048/3685246‑3685378 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |