| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,685,375 | C→T | 100% | pseudogene (747/1645 nt) pseudogene (747/984 nt) |
ilvG ← ilvG ← |
ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,685,375 | 0 | C | T | 100.0% | 212.6 / NA | 70 | pseudogene (747/1645 nt) pseudogene (747/984 nt) | ilvG ilvG | ECK3760:JW3740+JW3741:b4488; large subunit of acetolactate synthase II ECK3760:JW3740:b3767; acetolactate synthase II, large subunit, N‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (37/33); total (37/33) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTGCGCCCACGGCGATCAGCAGGTCACACTCCTGCACCGCGAAGTTTGCCGCTTTGGTGCCGTGCATCCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCGTACAGG > AP009048/3685308‑3685439 | gTGCACCCACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtccc < 1:4446540/71‑1 (MQ=255) cacccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtccc > 1:1957356/3‑68 (MQ=255) cacccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGc < 1:367418/69‑1 (MQ=255) cccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtcc < 1:3016720/65‑1 (MQ=255) cccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATtcc < 1:1460218/65‑1 (MQ=255) cccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCAt > 1:253580/1‑71 (MQ=255) cccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCAt < 1:653436/71‑1 (MQ=255) ccACGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCAta > 1:1103769/1‑70 (MQ=255) cGGCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGccc < 1:284041/71‑1 (MQ=255) ggCGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCa > 1:4001396/1‑65 (MQ=255) cGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGa < 1:4191826/71‑1 (MQ=37) cGATCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGa < 1:4166155/71‑1 (MQ=37) tCAGCAAGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAg > 1:4130429/1‑71 (MQ=37) cagcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAg > 1:3255395/1‑70 (MQ=37) agcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATa < 1:1503941/68‑1 (MQ=37) agcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTa < 1:1254994/71‑1 (MQ=37) agcaaGTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGAATGCCCAGATAGTa > 1:1594598/1‑71 (MQ=255) caagTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACg < 1:2131279/68‑1 (MQ=37) agTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGa < 1:4362147/70‑1 (MQ=38) agTCGCACTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGa > 1:3725805/2‑71 (MQ=38) cacTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGt > 1:2787170/1‑61 (MQ=38) acTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGa > 1:3874761/1‑56 (MQ=37) acTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCt < 1:4317234/71‑1 (MQ=39) acTCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCt > 1:2558482/1‑71 (MQ=39) tCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAAt < 1:2248599/67‑1 (MQ=38) tCCTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGc > 1:2631502/1‑71 (MQ=39) cTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCTCAGCATGCCCAGATAGTACGGATAAt > 1:1675272/1‑65 (MQ=255) cTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAAt > 1:1855484/1‑65 (MQ=38) cTGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCtt > 1:1295848/1‑71 (MQ=39) tGCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGc < 1:3926506/68‑1 (MQ=38) gCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAAt > 1:894781/1‑63 (MQ=38) gCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAAt > 1:3255422/1‑63 (MQ=38) gCACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAAt > 1:2676528/1‑63 (MQ=38) cACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTc < 1:3138828/69‑1 (MQ=38) cACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTc < 1:3026249/69‑1 (MQ=38) cACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTc < 1:1107820/69‑1 (MQ=38) cACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTc < 1:2319507/69‑1 (MQ=38) cACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTa > 1:469499/1‑71 (MQ=39) cACCGCGAAGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTa > 1:1946842/1‑71 (MQ=39) aaGTTCGCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGcc < 1:198385/71‑1 (MQ=39) aGTTCGCCTCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTa > 1:2103640/1‑63 (MQ=37) cgCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGcc > 1:1268391/2‑71 (MQ=255) cgCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGcc > 1:2413708/2‑71 (MQ=255) cgCCGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGc < 1:967351/69‑1 (MQ=39) ccGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCt > 1:380496/1‑71 (MQ=255) cGCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCtt > 1:2519762/1‑71 (MQ=255) gCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCtt < 1:4105679/52‑1 (MQ=37) gCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCttt > 1:278469/1‑71 (MQ=255) gCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCttt < 1:2495446/71‑1 (MQ=255) gCTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCttt < 1:3814974/71‑1 (MQ=255) cTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTc < 1:2340568/52‑1 (MQ=37) cTTTGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTc < 1:2345771/52‑1 (MQ=37) tttGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCttt > 1:1739560/1‑69 (MQ=255) tGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCa > 1:3000738/1‑69 (MQ=255) tGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCa < 1:3510689/69‑1 (MQ=255) tGGTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGc > 1:4614816/1‑71 (MQ=255) gTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCg < 1:3274555/70‑1 (MQ=255) gTGCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCACAGCCCTTTCAGCGt < 1:4248865/71‑1 (MQ=39) gCCATGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCGTa > 1:146208/1‑70 (MQ=255) ccatGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCt < 1:4520264/57‑1 (MQ=39) ccatGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCGTa > 1:3682824/4‑69 (MQ=255) ccatGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCGTa > 1:1431794/4‑69 (MQ=255) catGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCGTACAg < 1:3304335/69‑1 (MQ=255) catGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCGTACAg > 1:236081/3‑71 (MQ=255) catGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCGTACAg > 1:3716998/3‑71 (MQ=255) catGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCGTACAg > 1:2358779/3‑71 (MQ=255) atGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCGTAc < 1:3175422/67‑1 (MQ=255) atGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCGTACAgg > 1:382672/2‑71 (MQ=255) atGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCGTACAgg > 1:3007551/2‑71 (MQ=255) tGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGc < 1:4013840/38‑1 (MQ=255) tGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACt < 1:4492881/45‑1 (MQ=255) tGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCg < 1:4183654/64‑1 (MQ=255) tGCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCg < 1:2780308/64‑1 (MQ=255) gCATTCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAg > 1:725097/1‑52 (MQ=255) | GTGCGCCCACGGCGATCAGCAGGTCACACTCCTGCACCGCGAAGTTTGCCGCTTTGGTGCCGTGCATCCCCAGCATGCCCAGATAGTACGGATAATCTGCTTCTACTGCGCCCAGCCCTTTCAGCGTACAGG > AP009048/3685308‑3685439 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |