| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,733,358 | T→C | 8.7% | Y127H (TAC→CAC) | bglB → | cryptic phospho‑beta‑glucosidase B |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,733,358 | 0 | T | C | 8.7% | 120.8 / 4.3 | 46 | Y127H (TAC→CAC) | bglB | cryptic phospho‑beta‑glucosidase B |
| Reads supporting (aligned to +/- strand): ref base T (21/21); new base C (2/2); total (23/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.67e-01 | |||||||||||
GCGTTTTACGATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCATCG > AP009048/3733289‑3733418 | gCGTTTTACGATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTa < 1:2024847/71‑1 (MQ=255) cGTTTTACGATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTAc > 1:4060149/1‑71 (MQ=255) gTTTTACGATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTAc > 1:389862/1‑70 (MQ=255) gTTTTACGATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTAc > 1:2130883/1‑70 (MQ=255) ttACGATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCAtt > 1:3071559/1‑65 (MQ=255) ttACGATCGGCTGTTTGATAAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGa > 1:2769759/1‑69 (MQ=255) cGATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGc > 1:1306601/1‑71 (MQ=255) gATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGcc < 1:799538/71‑1 (MQ=255) cTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATgg < 1:3192661/71‑1 (MQ=255) tGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCa > 1:3799015/1‑65 (MQ=255) tttGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGc < 1:3470931/70‑1 (MQ=255) tgatgaAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGc > 1:521134/1‑68 (MQ=255) tgatgaAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTgg > 1:3905559/1‑71 (MQ=255) aaaTGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGaaa < 1:4127628/71‑1 (MQ=255) aaaTGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGaaa < 1:3778175/71‑1 (MQ=255) tGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATg > 1:4085106/1‑57 (MQ=255) tGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAAc < 1:3858332/71‑1 (MQ=255) gcgcAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTa > 1:539749/1‑71 (MQ=255) gcgcAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTa > 1:3205830/1‑71 (MQ=255) gcgcAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTa > 1:349735/1‑71 (MQ=255) gcAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTAcg < 1:2284390/71‑1 (MQ=255) cAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTAcgg < 1:431095/71‑1 (MQ=255) ggCGGGGATCAAGCCGCTGGTAACGTTATCCCAtt < 1:4046944/35‑1 (MQ=255) ggCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCa < 1:2967784/46‑1 (MQ=255) gCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGaaa > 1:1413077/1‑40 (MQ=255) gCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCt > 1:1170603/1‑53 (MQ=255) gCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTAcggcgg < 1:3931567/71‑1 (MQ=255) gCGGGGATCAAGCCGCTGGTAACGTTATCCCATCACGa < 1:4503777/38‑1 (MQ=255) cGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGt < 1:3317076/71‑1 (MQ=255) ggggATCAAGCCGCTGGTAACGTTATCCCATTACGAAATg < 1:2241669/40‑1 (MQ=255) ggggATCAAGCCGCTGGTAACGTTATCCCATCACGAAATGCCATATg < 1:391357/47‑1 (MQ=255) ggggATCAAGCCGCTGGTAACGTTATAACATTACGAAATg > 1:52443/1‑40 (MQ=255) gATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGc < 1:1799309/47‑1 (MQ=255) gATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTAc > 1:2902811/1‑61 (MQ=255) aTCAAGCCGCTGGTAACGTTATCCCATCACGAAATGCCATATGGGCTGGTGATAAACTACGGCGGTTGGGc > 1:720790/1‑71 (MQ=255) tCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCt < 1:2292555/71‑1 (MQ=255) tCAAGCCGCTGGTAACGTTATCCCATCACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGc > 1:4379588/1‑70 (MQ=255) aGCCGCTGGTAAGGTTATCCCATTACGAAATGCCATATGGGCTGGTGaaaaa > 1:1155024/1‑52 (MQ=255) gCCGCTGGTAACGTTATCCCATTACGAAATGCCata > 1:3706222/1‑36 (MQ=255) gCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATc > 1:134352/1‑71 (MQ=255) ccGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCTGTTGGGCTAATCg < 1:4057726/71‑1 (MQ=255) cTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAg < 1:3133239/70‑1 (MQ=255) tAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGaaa < 1:3675733/41‑1 (MQ=255) aaCGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCa > 1:4104870/1‑71 (MQ=255) gTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCATc > 1:4369642/1‑70 (MQ=255) gTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCATCg < 1:4150782/71‑1 (MQ=255) tCCCATTACGAAATGCCATATGGGCTTGTGAAAAAc < 1:98715/36‑1 (MQ=255) ccATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCATCg < 1:1180820/65‑1 (MQ=255) | GCGTTTTACGATCGGCTGTTTGATGAAATGGCGCAGGCGGGGATCAAGCCGCTGGTAACGTTATCCCATTACGAAATGCCATATGGGCTGGTGAAAAACTACGGCGGTTGGGCTAATCGAGCGGTCATCG > AP009048/3733289‑3733418 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |