| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,824,949 | A→G | 100% | E101E (GAA→GAG) | pyrE → | orotate phosphoribosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,824,949 | 0 | A | G | 100.0% | 216.8 / NA | 62 | E101E (GAA→GAG) | pyrE | orotate phosphoribosyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (32/30); total (32/30) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CCACAACCGCTGTGGCACTGGCGGAGCATCACGACCTGGACCTGCCGTACTGCTTTAACCGCAAAGAAGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGG > AP009048/3824882‑3825013 | ccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGc > 1:1877022/1‑70 (MQ=255)ccacTACCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCa < 1:45416/71‑1 (MQ=255) ctaCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:861457/69‑1 (MQ=255) taCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaa > 1:699391/2‑68 (MQ=255) aCCGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGa > 1:3531944/1‑71 (MQ=255) ccgccgTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGAc > 1:301170/1‑71 (MQ=255) cGCCGTGGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGAc > 1:2433211/1‑70 (MQ=255) ccgtgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCaaaa < 1:2300501/63‑1 (MQ=255) cgtgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGa > 1:787647/2‑66 (MQ=255) cgtgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACg > 1:3542628/2‑71 (MQ=255) tgGCGCTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGt < 1:1153678/71‑1 (MQ=255) gcgcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTg > 1:1464760/4‑70 (MQ=255) gcgcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTg < 1:4145690/67‑1 (MQ=255) gcTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGaa < 1:1666346/69‑1 (MQ=255) cTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAgg < 1:2756953/71‑1 (MQ=255) cTGGCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGA‑CACGGTg < 1:4227687/66‑1 (MQ=255) ggCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTg < 1:3488427/65‑1 (MQ=255) gCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcg < 1:4102944/70‑1 (MQ=255) gCGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcg < 1:6348/70‑1 (MQ=255) cGGAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAggcggc < 1:4521222/71‑1 (MQ=255) gAGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCaa > 1:2592744/1‑71 (MQ=255) aGCATCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAAt < 1:354690/71‑1 (MQ=255) aTCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtg < 1:580753/71‑1 (MQ=255) tCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtgg > 1:1549055/1‑71 (MQ=255) tCATGACCTTGACCTGCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtgg < 1:3171544/71‑1 (MQ=255) atgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCaaaa > 1:1827565/3‑44 (MQ=38) atgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGt > 1:779067/3‑53 (MQ=255) atgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggtt > 1:3935334/3‑71 (MQ=255) atgaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggtt > 1:180533/3‑71 (MQ=255) gaccttgacctgCCGTACTGCTTTAACCGCAAAGAGGCa < 1:4384097/39‑1 (MQ=38) accttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCaa < 1:4358095/61‑1 (MQ=255) accttgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggttgg < 1:4422715/70‑1 (MQ=255) accttgacctgCCGTAATGCTTTAACCGCAAAGAGGCAAAAGACCAc > 1:419034/1‑47 (MQ=38) tgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTa > 1:4434094/2‑68 (MQ=255) tgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAgcg > 1:3633362/2‑71 (MQ=255) tgacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACAACGGTGAAGGCGGCAATc > 1:4358478/2‑59 (MQ=39) gacctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAgc < 1:1223983/69‑1 (MQ=255) cctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggt > 1:1917802/1‑60 (MQ=255) cctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGt > 1:484505/1‑71 (MQ=255) ctgCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGtt < 1:4046602/71‑1 (MQ=255) gCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTa > 1:2787669/1‑70 (MQ=255) gCCGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTa > 1:4372421/1‑70 (MQ=255) ccGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTAc > 1:4409848/1‑70 (MQ=255) cGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACaa < 1:3609967/71‑1 (MQ=255) cGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACaa < 1:1195127/71‑1 (MQ=255) cGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACaa < 1:2786256/71‑1 (MQ=255) cGTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACaa < 1:4044171/71‑1 (MQ=255) gTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCaa < 1:1992013/48‑1 (MQ=255) gTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAg < 1:2575451/71‑1 (MQ=255) gTACTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAg < 1:74315/71‑1 (MQ=255) aCTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGa > 1:3067986/1‑71 (MQ=255) cTGCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGAc < 1:2534745/71‑1 (MQ=255) gCTTTAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATc > 1:665524/1‑45 (MQ=255) ttAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGt > 1:2692478/1‑70 (MQ=255) tAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGa > 1:455540/1‑64 (MQ=255) tAACCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTa < 1:1475214/70‑1 (MQ=255) aCCGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAAt > 1:1581771/1‑70 (MQ=255) aCCGCAAAGAGCCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTa < 1:1358585/68‑1 (MQ=255) ccGCAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggt > 1:2651042/1‑42 (MQ=255) cAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTgg > 1:4022381/1‑71 (MQ=255) cAAAGAGGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTgg > 1:679173/1‑71 (MQ=255) aaaGAGGCAAAAGACCACGGTGAAGGCGGCAATCtggttgg > 1:1033526/1‑41 (MQ=255) agaggCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTgg > 1:3602123/1‑68 (MQ=255) agaggCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTgg > 1:2251706/1‑68 (MQ=255) | CCACAACCGCTGTGGCACTGGCGGAGCATCACGACCTGGACCTGCCGTACTGCTTTAACCGCAAAGAAGCAAAAGACCACGGTGAAGGCGGCAATCTGGTTGGTAGCGCGTTACAAGGACGCGTAATGCTGG > AP009048/3824882‑3825013 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |