| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 3,915,031 | T→C | 12.8% | intergenic (+29/‑66) | ysaB → / → glyQ | hypothetical protein/glycine tRNA synthetase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 3,915,031 | 0 | T | C | 12.8% | 203.1 / 20.9 | 78 | intergenic (+29/‑66) | ysaB/glyQ | hypothetical protein/glycine tRNA synthetase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (32/36); new base C (4/6); total (36/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.45e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
TTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGAAATAATATGC > AP009048/3914962‑3915100 | ttGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGtat < 1:2498198/70‑1 (MQ=255) aCGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATc < 1:3020841/70‑1 (MQ=255) aCGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATc < 1:2266519/70‑1 (MQ=255) cgcgGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCtt < 1:2974097/71‑1 (MQ=255) cgcgGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCtt > 1:4125360/1‑71 (MQ=255) cgcgGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCtt > 1:4120195/1‑71 (MQ=255) cgGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTtcc < 1:505551/71‑1 (MQ=255) ggATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTtcca < 1:1685771/71‑1 (MQ=255) ggATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTtcca > 1:2208076/1‑71 (MQ=255) ggATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTtcca < 1:3949071/71‑1 (MQ=255) gCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAg < 1:4163553/71‑1 (MQ=255) gCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTtccatcca < 1:2102023/70‑1 (MQ=255) cATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTtcc < 1:1693318/63‑1 (MQ=255) aTTTTTTGCATCTTTCCATGCGTTAAGCCTTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCgg > 1:840226/1‑71 (MQ=255) aTTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCgg > 1:3845248/1‑71 (MQ=255) aTTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCgg < 1:1213083/71‑1 (MQ=255) ttttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCggg < 1:2981695/71‑1 (MQ=255) tttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCggg > 1:2089946/1‑70 (MQ=255) tttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCggg > 1:3540666/1‑70 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGTTTTTTCCCGTTTCGTACTGTATATCTtccatcca > 1:2409982/1‑64 (MQ=255) ttttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATc > 1:3386274/1‑55 (MQ=255) tttGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTtcca > 1:706760/1‑59 (MQ=255) tGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta > 1:181253/1‑68 (MQ=255) gCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGc < 1:3936129/62‑1 (MQ=255) cATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTg < 1:3147065/71‑1 (MQ=255) cATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTg < 1:3200928/71‑1 (MQ=255) aTCTTTCCATGCGTTAGGCCTTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGa < 1:2580096/71‑1 (MQ=255) aTCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGtat < 1:4281104/66‑1 (MQ=255) tCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGtat < 1:4198528/45‑1 (MQ=255) tCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTtccatcc > 1:2250638/1‑56 (MQ=255) tCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGAt > 1:2137977/1‑71 (MQ=255) cTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTATTGTATATCTTCAATCCAGCGGGTATACTGATc > 1:644062/1‑71 (MQ=255) cTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTatat > 1:695364/1‑46 (MQ=255) cTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATAc < 1:1684199/66‑1 (MQ=255) cTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATc > 1:721387/1‑71 (MQ=255) tttCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCtt > 1:4561659/1‑48 (MQ=255) tttCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGtata < 1:4288539/64‑1 (MQ=255) ttCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCgg > 1:1458285/1‑58 (MQ=255) ttCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCgg > 1:1746025/1‑58 (MQ=255) tCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGAt < 1:2945413/67‑1 (MQ=255) tCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGTATACTGATccc < 1:3701804/70‑1 (MQ=255) cATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTATTGTATATCTTCCATCCAGCGGGTATACTGATCcctt < 1:4189132/70‑1 (MQ=255) cATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCccttc < 1:4367778/71‑1 (MQ=255) cATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCccttc < 1:2965747/71‑1 (MQ=255) cATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCccttc < 1:2190694/71‑1 (MQ=255) aTGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCcctt < 1:4187160/69‑1 (MQ=255) cGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCccttcctt > 1:3962811/1‑70 (MQ=255) gTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCccttcctt > 1:4149394/1‑69 (MQ=255) ttAAGCCCTGCTTTTTCCCGTTTCGTATTGTATATCTtccatcca < 1:980797/45‑1 (MQ=255) ttAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCgg < 1:3750040/49‑1 (MQ=255) ttAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTaa < 1:2384387/71‑1 (MQ=255) tAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTaaa > 1:2062926/1‑71 (MQ=255) aaGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTaaa < 1:1373728/70‑1 (MQ=255) aaGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAAt > 1:347707/1‑71 (MQ=255) cccTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCca < 1:2171373/71‑1 (MQ=255) cccTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCca < 1:3126590/71‑1 (MQ=255) ccTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGta < 1:817097/46‑1 (MQ=255) ccTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCcac < 1:2565048/71‑1 (MQ=255) cTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCggg < 1:379539/43‑1 (MQ=255) tGCTTTTTCCCGTTTCGTATTGTATATCTTCCATCCAGCGGGTATACTGATc < 1:659840/52‑1 (MQ=255) tGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACt < 1:2438315/48‑1 (MQ=255) tGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCccttc > 1:642646/1‑57 (MQ=255) gCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCcctt > 1:841567/1‑55 (MQ=255) gCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCacac > 1:3335326/1‑70 (MQ=255) cTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGa > 1:4281289/1‑48 (MQ=255) tttttCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGtata > 1:3653650/1‑43 (MQ=255) tttttCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCacac > 1:40379/1‑68 (MQ=255) tttCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGtata > 1:3690942/1‑41 (MQ=255) tCCCGTTTCGTACTGTACATCTTCCATCCAGCGGGTATAc > 1:250096/1‑40 (MQ=255) ccGTTTCGTATTGTATATCTTCCATCCAGCGGGTATACTGa < 1:1145073/41‑1 (MQ=255) ccGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAg < 1:442186/70‑1 (MQ=255) gTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTa > 1:3960734/1‑51 (MQ=255) tttCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCccttcc > 1:3188831/1‑46 (MQ=255) tttCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTa > 1:1107257/1‑62 (MQ=255) tttCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCAc > 1:3359569/1‑70 (MQ=255) tttCGTACTGTACATCTTCCATCCAGCGGGTATACTGATCccttcctt < 1:4127498/48‑1 (MQ=255) tCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGa < 1:2107128/70‑1 (MQ=255) tCGTACTGTACATCTTCCATCCAGCGGGTATACTGATCccttc > 1:3305453/1‑43 (MQ=255) tCGTACTGTACATCTTCCATCCAGCGGGTATACTGATCccttc > 1:2966415/1‑43 (MQ=255) cGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGa < 1:650441/69‑1 (MQ=255) tACTGTACATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGAaata < 1:1820406/71‑1 (MQ=255) atatCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCca > 1:663954/1‑45 (MQ=255) atatCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGAAATAATATg > 1:1878885/1‑70 (MQ=255) tatCTTCCATCCAGCGGGTATACTGATCCCTTCCttt < 1:1048801/37‑1 (MQ=255) tatCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGGAATAatat > 1:3023890/1‑68 (MQ=255) tatCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGAAATAatat < 1:2439391/68‑1 (MQ=255) tatCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGAAATAATATGc > 1:2803874/1‑70 (MQ=255) | TTGACGCGGATGGCCATTTTTTGCATCTTTCCATGCGTTAAGCCCTGCTTTTTCCCGTTTCGTACTGTATATCTTCCATCCAGCGGGTATACTGATCCCTTCCTTTAAATCCACACGTATCCAGCACGAAATAATATGC > AP009048/3914962‑3915100 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |