| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 4,027,857 | A→G | 20.0% | L232L (TTG→CTG) | yhhT ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 4,027,857 | 0 | A | G | 20.0% | 59.6 / 11.5 | 30 | L232L (TTG→CTG) | yhhT | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base A (10/14); new base G (4/2); total (14/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.78e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
ACAGCAGCACCTGAATCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGCTCCAGCCCCAGCCAGACGATGACA > AP009048/4027788‑4027927 | aCAGCAGCACCTGAATCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCaaa < 1:697009/71‑1 (MQ=255)aCAGCAGCACCTGAATCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCaaa < 1:4236110/71‑1 (MQ=255)aCAGCAGCACCTGAATCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCaaa < 1:4200481/71‑1 (MQ=255)aCAGCAGCACCTGAATCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCaa > 1:1872572/1‑70 (MQ=255)aCAGCAGCACCTGAATCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCaa > 1:3259429/1‑70 (MQ=255) gcagcaCCTGAATCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAAc < 1:305931/71‑1 (MQ=255) agcaCCTGAATCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGc < 1:2683627/71‑1 (MQ=255) agcaCCTGAATCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGc < 1:3217222/71‑1 (MQ=255) ccTGAATCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAg > 1:1383434/1‑70 (MQ=255) gAATCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTACt > 1:1724691/1‑71 (MQ=255) aTCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGt < 1:1951011/66‑1 (MQ=255) gCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTACTGCCCACATc < 1:2155699/71‑1 (MQ=255) gCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTACTGCCCACATc < 1:243602/71‑1 (MQ=255) cggcggAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAg > 1:1413769/1‑52 (MQ=255) ggcggAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCaa < 1:2667752/71‑1 (MQ=255) gcggAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCaa < 1:3809793/41‑1 (MQ=255) cggAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAAc > 1:2550605/1‑71 (MQ=255) gAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCa > 1:2003386/1‑46 (MQ=255) aaaTTACCGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAGTACTGCCCACATCAGCGCAAACTg > 1:1017173/1‑70 (MQ=255) aTTACCGCGCCGATATTGGGCTCGTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCAc > 1:2332097/1‑71 (MQ=255) aTTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTACTg > 1:3733914/1‑49 (MQ=255) tACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCAccc > 1:979584/1‑71 (MQ=255) aCCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCAccc < 1:4542924/70‑1 (MQ=255) ccGCGCCGATATTGGGCACGTAGTTGAGCAGAAACGCCAGTACTGCCCACATCAGCGCAAACTGCAcccc > 1:352459/1‑70 (MQ=255) gcgcCGATATTGGGCACGTAGTTGAGCAGAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCAt < 1:1986081/71‑1 (MQ=255) cgcCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATc < 1:3942581/71‑1 (MQ=255) ccGATATTGGGCACGTAGTTGAGCAGAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAg > 1:3813558/1‑71 (MQ=255) atatTGGGCACGTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAGTGCAAACTGCACCCCCATCAGCTc < 1:1175079/71‑1 (MQ=255) aCGTAGTTGAGCAGAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGCTccagccccag > 1:2123163/1‑71 (MQ=255) aCGTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGCTccagccccag < 1:4431995/71‑1 (MQ=255) aCGTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCAATCAGCt > 1:501837/1‑61 (MQ=255) aGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCa < 1:2823403/54‑1 (MQ=255) atGAGCAGAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGCTCCAGCCCCAGCCAGAc < 1:1864637/70‑1 (MQ=255) gAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGCTCCAGCCCCAGCCAGACg < 1:1264898/70‑1 (MQ=255) gAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGCTCCAGCCCCAGCCAGACg < 1:2843928/70‑1 (MQ=255) gCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGCTCCAGCCCCAGCCAGACGAt > 1:4365293/1‑70 (MQ=255) aaaaaCGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGCTCCAGCCCCAGCCAGACGATGac < 1:205071/71‑1 (MQ=255) aaaaaCGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGCTCCAGCCCCAGCCAGACGATGa > 1:2871073/1‑70 (MQ=255) aaaaCGCCAGTACTGCCCACATCAGCGCAAACTGCa < 1:2458417/36‑1 (MQ=255) aaaaCGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCa > 1:1196597/1‑42 (MQ=255) aaaaCGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCa > 1:3056793/1‑45 (MQ=255) aaaaCGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGCTCCAGCCCCAGCCAGACGATGaca > 1:2094150/1‑71 (MQ=255) | ACAGCAGCACCTGAATCATTGGCGGTACGGCGGAAATTACCGCGCCGATATTGGGCACGTAGTTGAGCAAAAACGCCAGTACTGCCCACATCAGCGCAAACTGCACCCCCATCAGCTCCAGCCCCAGCCAGACGATGACA > AP009048/4027788‑4027927 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |