| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 4,418,870 | A→G | 10.8% | *388* (TAA→TGA) | yjfC → | predicted synthetase/amidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 4,418,870 | 0 | A | G | 10.8% | 263.7 / 22.6 | 102 | *388* (TAA→TGA) | yjfC | predicted synthetase/amidase |
| Reads supporting (aligned to +/- strand): ref base A (53/38); new base G (3/8); total (56/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.15e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
ATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATT > AP009048/4418803‑4418938 | aTCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAg > 1:2598415/1‑70 (MQ=255) ccGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAAt > 1:3909737/1‑71 (MQ=255) ccGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAAt > 1:2043466/1‑71 (MQ=255) cGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAAt < 1:2745690/70‑1 (MQ=255) cGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATg > 1:682542/1‑71 (MQ=255) gTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGt < 1:500590/71‑1 (MQ=255) gTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGt < 1:4469668/71‑1 (MQ=255) tGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGtt > 1:4426528/1‑71 (MQ=255) aaGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATGAGAATGtttt < 1:3739680/71‑1 (MQ=255) aaGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGtttt < 1:2972636/71‑1 (MQ=255) aaGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGttt < 1:4270939/70‑1 (MQ=255) aaGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGttt < 1:4156613/70‑1 (MQ=255) aaGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGttt > 1:1721157/1‑70 (MQ=255) aaGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGttt > 1:4481407/1‑70 (MQ=255) aGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGttt > 1:1680399/1‑69 (MQ=255) aGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGttt > 1:4570344/1‑69 (MQ=255) tAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGc > 1:4317576/1‑70 (MQ=255) aaCACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCaa > 1:724589/1‑71 (MQ=255) cacacTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAAtc > 1:4245129/1‑71 (MQ=255) acacTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTa > 1:121076/1‑64 (MQ=255) acacTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAAtct > 1:2146442/1‑71 (MQ=255) acTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATctct > 1:3146708/1‑71 (MQ=255) acTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATctct > 1:1124163/1‑71 (MQ=255) acTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATctct > 1:1776696/1‑71 (MQ=255) cTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATctct > 1:3898847/1‑70 (MQ=255) tGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCttt > 1:3454617/1‑71 (MQ=255) gATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATAGCTGGATAAGAATGTTTTAGCAATCTCTTTc > 1:3199639/1‑71 (MQ=255) aTCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCttt < 1:1348076/69‑1 (MQ=255) aTCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCt > 1:623960/1‑71 (MQ=255) aTCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCt > 1:3427579/1‑71 (MQ=255) tCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTg > 1:2454578/1‑71 (MQ=255) tCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTg < 1:41566/71‑1 (MQ=255) tCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTg > 1:3117936/1‑71 (MQ=255) aCCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGt < 1:4219492/70‑1 (MQ=255) aCCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGt < 1:4358745/70‑1 (MQ=255) ccAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAAt < 1:754898/48‑1 (MQ=255) cAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCAt > 1:1501562/1‑71 (MQ=255) aaaGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCAt > 1:3394734/1‑70 (MQ=255) aaGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATaa < 1:3079992/41‑1 (MQ=255) aaGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGTCATg > 1:3443679/1‑70 (MQ=255) aaGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATg > 1:1291932/1‑70 (MQ=255) aaGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATg > 1:2923745/1‑70 (MQ=255) gACAACTCACGTTTCGTTCCGCATTACATTGCTGGATaa > 1:4424881/1‑39 (MQ=255) cacCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATc > 1:1032632/1‑70 (MQ=255) acCTCACGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCa < 1:1363758/71‑1 (MQ=255) acCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGaa < 1:3106716/67‑1 (MQ=255) acCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAAt > 1:1001919/1‑68 (MQ=255) ccTCACGTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCAt < 1:1042843/71‑1 (MQ=255) cTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGc < 1:3049471/46‑1 (MQ=255) cTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATg > 1:3382079/1‑71 (MQ=255) cTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATg > 1:2255869/1‑71 (MQ=255) tCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCAt > 1:4116725/1‑69 (MQ=255) cACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATgg > 1:3785181/1‑70 (MQ=255) aCGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAAtctc > 1:3749269/1‑49 (MQ=255) aCGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATgg < 1:4068602/69‑1 (MQ=255) aCGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATgg < 1:2853026/69‑1 (MQ=255) aCGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATgg > 1:4221301/1‑69 (MQ=255) aCGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATg < 1:4085683/68‑1 (MQ=255) aCGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCa < 1:4301884/71‑1 (MQ=255) cGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATgg < 1:1128639/68‑1 (MQ=255) cGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAg > 1:3900741/1‑71 (MQ=255) gTTTCGTTCCGCATTACATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGt < 1:3047508/71‑1 (MQ=255) gTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGt < 1:4296519/71‑1 (MQ=255) tttCGTTCCGCATTACATTGCTGGATAAGAATGttt < 1:222042/36‑1 (MQ=255) tttCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAg < 1:4279538/39‑1 (MQ=255) tttCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAg < 1:3375219/39‑1 (MQ=255) tttCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGt < 1:1420343/53‑1 (MQ=255) tttCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCAt < 1:1166404/64‑1 (MQ=255) ttCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGa > 1:2158777/1‑71 (MQ=255) tCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAAtctc > 1:3602769/1‑44 (MQ=255) tCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCa > 1:2573499/1‑66 (MQ=255) tCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGAc > 1:2556926/1‑71 (MQ=255) gTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCttt > 1:2848397/1‑45 (MQ=255) gTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCa < 1:1145277/71‑1 (MQ=255) gTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCa < 1:1722485/71‑1 (MQ=255) tCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATgg > 1:3927443/1‑60 (MQ=255) ccGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTg > 1:1358503/1‑64 (MQ=255) ccGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGa > 1:786271/1‑65 (MQ=255) ccGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATAc > 1:743907/1‑71 (MQ=255) ccGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATAc > 1:321286/1‑71 (MQ=255) ccGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATAc > 1:2044966/1‑71 (MQ=255) cATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCAt < 1:4415914/54‑1 (MQ=255) ttACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTg < 1:4071926/40‑1 (MQ=255) ttACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATg < 1:423371/71‑1 (MQ=255) tACATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGTCATGa > 1:2384842/1‑45 (MQ=255) aCATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAAt > 1:3080618/1‑46 (MQ=255) aCATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATgg < 1:2327810/70‑1 (MQ=255) aCATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGt > 1:2220700/1‑71 (MQ=255) cATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTg < 1:4463475/71‑1 (MQ=255) cATTGCTGGATGAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATCGCAGTGACCATACTAATGGTg < 1:319846/71‑1 (MQ=255) cATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGt < 1:853687/70‑1 (MQ=255) aTTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGt < 1:4525908/69‑1 (MQ=255) cTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCa > 1:3702032/1‑55 (MQ=255) ggATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATg < 1:3737288/61‑1 (MQ=255) gATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCAt > 1:398870/1‑71 (MQ=255) aTAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCa < 1:4406833/51‑1 (MQ=255) aTAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGAc < 1:48320/64‑1 (MQ=255) aTAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGAc < 1:1528715/64‑1 (MQ=255) tAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATg > 1:811865/1‑58 (MQ=255) tAAGAATGTTTTAGCAATCACTTTCTGTCATGAATCCATGGCAGTGACCATACTACTGGTGACTGCCAtt < 1:1013381/70‑1 (MQ=255) | ATCCGTGAAGATAACACACTGATCACCAAAGACACCTCACGTTTCGTTCCGCATTACATTGCTGGATAAGAATGTTTTAGCAATCTCTTTCTGTCATGAATCCATGGCAGTGACCATACTAATGGTGACTGCCATT > AP009048/4418803‑4418938 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |