| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 556,858 | A→T | 100% | L36Q (CTG→CAG) | folD ← | bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 556,858 | 0 | A | T | 100.0% | 171.1 / NA | 57 | L36Q (CTG→CAG) | folD | bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase and 5,10‑methylene‑tetrahydrofolate cyclohydrolase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (31/26); total (31/26) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCG > AP009048/556791‑556919 | acaAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTc > 1:67199/1‑71 (MQ=255) caAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc < 1:793986/71‑1 (MQ=255) caAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc < 1:2483997/71‑1 (MQ=255) caAGCCTTGCCTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTcc > 1:3540652/1‑71 (MQ=255) aaGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCt < 1:2900049/71‑1 (MQ=255) aaGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCt < 1:2041473/71‑1 (MQ=255) gCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGccc > 1:818199/1‑71 (MQ=255) gCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGccc > 1:2172357/1‑71 (MQ=255) cGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCg > 1:4458730/1‑71 (MQ=255) gTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCg > 1:680131/1‑70 (MQ=255) ttgcttgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAg > 1:4391781/1‑71 (MQ=255) ttgcttgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAg > 1:4290539/1‑71 (MQ=255) ttgcttgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAg > 1:2045748/1‑71 (MQ=255) cttgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTcc > 1:3433510/1‑71 (MQ=255) ctcgcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTcc < 1:979992/68‑1 (MQ=255) gcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTg > 1:373912/1‑55 (MQ=255) gcGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGc > 1:3196740/1‑71 (MQ=255) gACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGt > 1:1487384/1‑55 (MQ=255) cATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc > 1:3182959/1‑70 (MQ=255) aTAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGt < 1:2678282/61‑1 (MQ=255) tAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGc < 1:3395162/68‑1 (MQ=255) tAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAAt < 1:4122691/71‑1 (MQ=255) tttGCGATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAcgc < 1:1736374/71‑1 (MQ=255) gATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCt < 1:2348084/39‑1 (MQ=255) gATGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTg < 1:4062036/71‑1 (MQ=255) aTGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTg > 1:786940/1‑70 (MQ=255) aTGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTg > 1:2373789/1‑70 (MQ=255) tGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGaa > 1:1333903/1‑71 (MQ=255) tGCAGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGaa > 1:507699/1‑71 (MQ=255) aGGGTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACtt < 1:802415/71‑1 (MQ=255) gggTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttt < 1:1816462/71‑1 (MQ=255) gggTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttt < 1:3411268/71‑1 (MQ=255) gggTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttt < 1:3685920/71‑1 (MQ=255) ggTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACtttt > 1:3121160/1‑71 (MQ=255) gTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGATCttttt < 1:594670/71‑1 (MQ=255) gTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttttt < 1:613458/71‑1 (MQ=255) gTTACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttttt < 1:3274468/71‑1 (MQ=255) ttACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCgg < 1:4611099/46‑1 (MQ=255) ttACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGaa < 1:3075430/64‑1 (MQ=255) ttACTACCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTg < 1:3182273/71‑1 (MQ=255) tactacCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACttttt > 1:3490376/1‑69 (MQ=255) actacCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGa < 1:445848/70‑1 (MQ=255) ctacCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATAcg > 1:1636078/1‑53 (MQ=255) ctacCCACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACt > 1:3903581/1‑63 (MQ=255) cccACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACgcgc > 1:1065268/1‑53 (MQ=255) cccACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAAc < 1:1808926/71‑1 (MQ=255) cccACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAAc > 1:4004812/1‑71 (MQ=255) cccACCAGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACCCGCCTGAACTTTTTGAGCAAc < 1:3034453/71‑1 (MQ=255) caccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACtt < 1:3230080/71‑1 (MQ=255) ccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCt > 1:1406205/1‑38 (MQ=255) ccaGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCa < 1:1033827/71‑1 (MQ=255) ccaGCAAAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCt > 1:114/1‑51 (MQ=255) caGCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACtt > 1:2148184/1‑68 (MQ=255) gCACAACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCagag > 1:4547808/1‑71 (MQ=255) acaACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAgcg > 1:3582471/1‑71 (MQ=255) caACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAgcg > 1:4340827/1‑70 (MQ=255) caACGGCCTGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAgcg > 1:2114274/1‑70 (MQ=255) | ACAAGCCTTGCGTTTGCTTGCGACATAAATTTGCGATGCAGGGTTACTACCCACCAGCACAACGGCCAGTCCTGGTGCCCGCAGTCCGGCTGCAATACGCGCCTGAACTTTTTGAGCAACTTCAGAGCG > AP009048/556791‑556919 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |