| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 702,273 | C→T | 14.6% | D301N (GAT→AAT) | nagA ← | N‑acetylglucosamine‑6‑phosphate deacetylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 702,273 | 0 | C | T | 14.6% | 219.6 / 25.2 | 82 | D301N (GAT→AAT) | nagA | N‑acetylglucosamine‑6‑phosphate deacetylase |
| Reads supporting (aligned to +/- strand): ref base C (32/38); new base T (4/8); total (36/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01 | |||||||||||
CAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGC > AP009048/702203‑702342 | cAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATc > 1:3771806/1‑71 (MQ=255)cAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATc > 1:1897041/1‑71 (MQ=255)cAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATc > 1:851715/1‑71 (MQ=255) aaTGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCAAcc < 1:4128288/71‑1 (MQ=255) tGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCcac < 1:1014393/71‑1 (MQ=255) cGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTcacaca < 1:2041573/70‑1 (MQ=255) cGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCcacaca > 1:3442048/1‑70 (MQ=255) gACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACaaa < 1:2896943/71‑1 (MQ=255) gACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACaa < 1:1599693/70‑1 (MQ=255) gACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACaa < 1:2982613/70‑1 (MQ=255) aCCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAg < 1:1106396/71‑1 (MQ=255) aCCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAg > 1:808162/1‑71 (MQ=255) ccAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATc > 1:3964574/1‑61 (MQ=255) ccAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATc > 1:3610487/1‑61 (MQ=255) cAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTc > 1:2962538/1‑71 (MQ=255) cAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTc > 1:2134104/1‑71 (MQ=255) aGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTcc < 1:1711164/71‑1 (MQ=255) aGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTcc < 1:3139198/71‑1 (MQ=255) gATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATc < 1:587848/58‑1 (MQ=255) gATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCg > 1:1217132/1‑71 (MQ=255) acgcacgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTa < 1:4427572/70‑1 (MQ=255) cgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGgtag < 1:4191800/71‑1 (MQ=255) cgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGgtag > 1:508452/1‑71 (MQ=255) cgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGgtag < 1:4396341/71‑1 (MQ=255) ttCAATCATGGTTAAGGATGCACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGtata > 1:513618/1‑71 (MQ=255) ttCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGt < 1:2835763/59‑1 (MQ=255) tCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTAc < 1:2769297/61‑1 (MQ=255) tCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGgta > 1:3793706/1‑65 (MQ=255) cAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGtata < 1:2136866/69‑1 (MQ=255) cAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTatat > 1:873120/1‑70 (MQ=255) cAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTatat > 1:3757602/1‑70 (MQ=255) cAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTATAtt > 1:3534682/1‑71 (MQ=255) cAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTa > 1:1243129/1‑59 (MQ=255) cAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTa > 1:715192/1‑59 (MQ=255) cAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTatat < 1:1318070/70‑1 (MQ=255) cAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTatat > 1:2863980/1‑70 (MQ=255) cAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTatat < 1:3516507/70‑1 (MQ=255) cAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATAtt > 1:3345608/1‑71 (MQ=255) cAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATAtt > 1:3510797/1‑71 (MQ=255) aaTCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGtata < 1:1288199/68‑1 (MQ=255) aaTCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATAtt < 1:4022566/70‑1 (MQ=255) aaTCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTg < 1:3027058/71‑1 (MQ=255) aaTCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTg > 1:447584/1‑71 (MQ=255) aaTCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTg < 1:457311/71‑1 (MQ=255) aaTCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTg < 1:2115765/71‑1 (MQ=255) tCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACaa < 1:2700942/47‑1 (MQ=255) tCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAg < 1:881280/49‑1 (MQ=255) tCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGtt < 1:4101109/71‑1 (MQ=255) cATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACaaa > 1:1685142/1‑47 (MQ=255) cATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCg > 1:213624/1‑52 (MQ=255) cATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGttt > 1:871229/1‑71 (MQ=255) aTGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGttt > 1:1095591/1‑70 (MQ=255) ggTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGgtagta < 1:3690028/60‑1 (MQ=255) ttAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGgta < 1:4385624/55‑1 (MQ=255) ttAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTAccc > 1:3455401/1‑71 (MQ=255) ggATGAACCGCTTAGCGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCa > 1:4427472/1‑70 (MQ=255) ggATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGgta < 1:628847/51‑1 (MQ=255) ggATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCa > 1:1742018/1‑70 (MQ=255) gATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGgta < 1:1079987/50‑1 (MQ=255) aTGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACgg > 1:2784310/1‑47 (MQ=255) aTGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCaaa > 1:4361737/1‑70 (MQ=255) aaCCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATAtt > 1:95402/1‑53 (MQ=255) aaCCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATg < 1:413541/71‑1 (MQ=255) aCCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGa < 1:2887237/71‑1 (MQ=255) ccGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGgtagta < 1:2714654/47‑1 (MQ=255) ccGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTg > 1:1779648/1‑52 (MQ=255) ccGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGaa < 1:3427549/71‑1 (MQ=255) cGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTa > 1:1969202/1‑56 (MQ=255) gCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACt < 1:2133658/71‑1 (MQ=255) cTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTAc < 1:696970/55‑1 (MQ=255) cTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTg < 1:2540826/71‑1 (MQ=255) tAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGtt > 1:97242/1‑71 (MQ=255) aaCGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGt < 1:3766715/69‑1 (MQ=255) aaCGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGtttt < 1:2137489/50‑1 (MQ=255) aaCGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGt < 1:2490052/69‑1 (MQ=255) cGTACCGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCaa > 1:4470578/1‑71 (MQ=255) tACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATg < 1:3613012/71‑1 (MQ=255) ccGTTCTCATTCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGt < 1:2534027/70‑1 (MQ=255) ccGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGtt > 1:3705239/1‑71 (MQ=255) ccGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGt < 1:1803912/70‑1 (MQ=255) ccGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGt < 1:2581373/70‑1 (MQ=255) ttCTCATTCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCa < 1:1908090/63‑1 (MQ=255) ctcATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGCACTGTTCAATGTTGGCAc > 1:218410/1‑71 (MQ=255) tcATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGt < 1:1882767/64‑1 (MQ=255) aTCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCaaa < 1:2629557/45‑1 (MQ=255) aTCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCAc > 1:4616404/1‑68 (MQ=255) aTCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACctg > 1:919799/1‑71 (MQ=255) aTCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACct < 1:2106205/70‑1 (MQ=255) tCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACctgc > 1:1875206/1‑71 (MQ=255) | CAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGC > AP009048/702203‑702342 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |