| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 1,393,837 | C→T | 12.1% | R45* (CGA→TGA) | ycjZ → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 1,393,837 | 0 | C | T | 12.1% | 166.2 / 11.7 | 58 | R45* (CGA→TGA) | ycjZ | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (24/27); new base T (4/3); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01 | |||||||||||
ACTCGTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCATCTTTT > AP009048/1393768‑1393907 | aCTCGTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAAc < 1:745430/70‑1 (MQ=255) cTCGTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGa > 1:605189/1‑71 (MQ=255) gTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTg > 1:2953366/1‑71 (MQ=255) gTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCATATAGAAGAACGATTg > 1:3009926/1‑71 (MQ=255) tGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTgg < 1:1958384/71‑1 (MQ=255) cagcagCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGa < 1:3008367/71‑1 (MQ=255) gcagcCCGCGTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGAtt < 1:1970359/71‑1 (MQ=255) gcagcCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGAt > 1:4263437/1‑70 (MQ=255) agcCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGc > 1:1245680/1‑71 (MQ=255) cGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTg > 1:538743/1‑60 (MQ=255) gCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGc < 1:1151499/66‑1 (MQ=255) ccTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTc > 1:3528328/1‑71 (MQ=255) ccTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTc < 1:4394727/71‑1 (MQ=255) cTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTgg > 1:3583302/1‑58 (MQ=255) cTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGAtt < 1:478519/62‑1 (MQ=255) tGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTc < 1:3145703/69‑1 (MQ=255) aGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTg < 1:3891504/69‑1 (MQ=255) aGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGa < 1:598643/70‑1 (MQ=255) cATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACg < 1:1574382/48‑1 (MQ=255) cATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGAcgc > 1:1922838/1‑71 (MQ=255) cATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGAcgc > 1:3605132/1‑71 (MQ=255) aTGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGAcgcg < 1:1010971/71‑1 (MQ=255) tGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGAcgcg > 1:3059807/1‑70 (MQ=255) ggCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGAcgcg < 1:1922701/69‑1 (MQ=255) cgcAGTCAGCTTTAAGCCAGATAATGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAAcc < 1:1675220/71‑1 (MQ=255) aaGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCAc > 1:1286503/2‑71 (MQ=255) tCAGCTTTAAGCCAGATAGTGCTTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCAcgcg > 1:1583828/1‑71 (MQ=255) tCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTgg < 1:3857891/43‑1 (MQ=255) tCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCAcgcg < 1:257132/71‑1 (MQ=255) tCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCAcgcg < 1:3412872/71‑1 (MQ=255) tCAGCTTTAAGCCAGATAGTGCATCGTATAGAAGAACGATTGGGAtt < 1:3888824/47‑1 (MQ=255) aGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGa < 1:3494731/56‑1 (MQ=255) aGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAAc > 1:3086180/1‑63 (MQ=255) cTTTAAGCCAGATAGTGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGc > 1:3948845/1‑71 (MQ=255) tttAAGCCAGATAGTGCGTCGTATAGAAGAACGATTgg > 1:2119235/1‑38 (MQ=255) ttAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGaa > 1:3407926/1‑58 (MQ=255) tAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCg < 1:1403593/69‑1 (MQ=255) aGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCgttg < 1:3790927/70‑1 (MQ=255) aGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCgttg < 1:3872361/70‑1 (MQ=255) gCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCt < 1:4188103/46‑1 (MQ=255) aGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTcc > 1:2340743/1‑70 (MQ=255) gATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAAc > 1:164256/1‑51 (MQ=255) aTAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAAc > 1:2411292/1‑71 (MQ=255) gTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGa < 1:3459776/45‑1 (MQ=255) tGCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACgcgc < 1:4501955/53‑1 (MQ=255) tGCGTCGTATAGAAGAACGATTGGGATTGCGGCtt > 1:84972/1‑35 (MQ=255) tGCGGCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTTTTCCAACTg < 1:2366836/69‑1 (MQ=255) gCGTCGTATAGAAGAATGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAg > 1:3612227/1‑71 (MQ=255) gtATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGc < 1:3546373/71‑1 (MQ=255) taGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGa < 1:1896277/70‑1 (MQ=255) gaagaaCGATTGGGATTGCGGCTTCTGACGCGAACCAc < 1:2242579/38‑1 (MQ=255) gaagaaCGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGc < 1:3625682/70‑1 (MQ=255) gaagaaCGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCa > 1:3584588/1‑71 (MQ=255) gaagaaCGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCa > 1:2060491/1‑71 (MQ=255) agaaCGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCATc > 1:206973/1‑71 (MQ=255) gaaCGATTGTGATTGCGGCTTCTGACGCGAACCACGCGCAGCgttgtt < 1:3342964/48‑1 (MQ=255) gaaCGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCATCt > 1:3500657/1‑71 (MQ=255) gaaCGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCATCt > 1:135031/1‑71 (MQ=255) aCGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCATCttt > 1:1319272/1‑71 (MQ=255) cGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCATCtttt < 1:3839186/71‑1 (MQ=255) cGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCATCtttt < 1:3238419/71‑1 (MQ=255) | ACTCGTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGTCGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCAACTGAAGCGGGCGAGCATCTTTT > AP009048/1393768‑1393907 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |