Predicted mutation
evidence seq id position mutation freq annotation gene description
RA AP009048 71,082 T→C 48.2% I232I (ATT→ATC araC → DNA‑binding transcriptional dual regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP00904871,0820TC48.2% 2.3 / 263.4 170I232I (ATT→ATCaraCDNA‑binding transcriptional dual regulator
Reads supporting (aligned to +/- strand):  ref base T (45/43);  new base C (37/45);  total (82/88)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.37e-01

Only 100 of 171 total aligned reads displayed.

GTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTT  >  AP009048/71015‑71149
                                                                   |                                                                   
gTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGt                                                                  >  1:1332330/1‑71 (MQ=255)
 tCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAgcc                                                                 <  1:1920693/71‑3 (MQ=255)
 tCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTc                                                                 <  1:4180304/71‑1 (MQ=255)
   tGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAg                                                               >  1:2662461/1‑71 (MQ=255)
    gTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAgg                                                              >  1:888766/1‑71 (MQ=255)
     tCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAgg                                                              <  1:55440/70‑1 (MQ=255)
     tCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAg                                                               <  1:3323880/69‑1 (MQ=255)
     tCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGc                                                             <  1:2549851/71‑1 (MQ=255)
      cacaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGc                                                             >  1:261851/1‑70 (MQ=255)
       acaTCTTTTCCGCCAGCAGTTAGGTATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCg                                                            <  1:2039587/70‑1 (MQ=255)
       acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAg                                                               >  1:2921102/1‑67 (MQ=255)
       acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCg                                                            >  1:2159883/1‑70 (MQ=255)
       acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCg                                                            <  1:4000147/70‑1 (MQ=255)
       acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCg                                                            >  1:4364944/1‑70 (MQ=255)
       acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCg                                                            >  1:1307785/1‑70 (MQ=255)
       acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGa                                                           <  1:4297047/71‑1 (MQ=255)
       acaTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGACGACCAACGTATCAGCCAGGc                                                             >  1:4470764/1‑69 (MQ=255)
        caTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAgcc                                                                 <  1:2561675/64‑3 (MQ=255)
         aTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAgg                                                              <  1:50516/66‑1 (MQ=255)
         aTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGaa                                                          >  1:4447731/1‑70 (MQ=255)
         aTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAg                                                         >  1:3649738/1‑71 (MQ=255)
         aTCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAg                                                         >  1:2114860/1‑71 (MQ=255)
           cTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAg                                                         >  1:3806362/1‑69 (MQ=255)
            ttttCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctg                                                      >  1:4371961/1‑71 (MQ=255)
            ttttCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTc                                                                 >  1:695760/1‑60 (MQ=255)
            ttttCCGCCAGCAGTTAGGGATTAGCATCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctg                                                      >  1:2010398/1‑71 (MQ=255)
              ttCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc                                                     <  1:3475473/70‑1 (MQ=255)
              ttCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAgctgc                                                     <  1:161619/70‑1 (MQ=255)
                ccGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCtt                                                   <  1:3032345/70‑1 (MQ=255)
                ccGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtt                                                   <  1:1480805/70‑1 (MQ=255)
                ccGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtt                                                   <  1:289941/70‑1 (MQ=255)
                ccGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCCCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtt                                                   <  1:1403365/70‑1 (MQ=255)
                  gCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTg                                                <  1:1990930/71‑1 (MQ=255)
                    cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg                                              <  1:4079085/71‑1 (MQ=255)
                    cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg                                              <  1:3186857/71‑1 (MQ=255)
                    cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAg                                              <  1:4285127/71‑1 (MQ=255)
                    cagcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTg                                                <  1:702846/69‑1 (MQ=255)
                      gcagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGa                                                           >  1:3819137/1‑56 (MQ=255)
                       cagTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCa                                            >  1:3875242/1‑70 (MQ=255)
                         gTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCAc                                           >  1:3158009/1‑69 (MQ=255)
                           tAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTAcc                                       >  1:4102953/1‑71 (MQ=255)
                            aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGc                                                             >  1:242759/1‑48 (MQ=255)
                            aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCaccacc                                       <  1:4069655/70‑1 (MQ=255)
                            aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCaccacc                                       <  1:3014456/70‑1 (MQ=255)
                            aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCaccacc                                       <  1:3125651/70‑1 (MQ=255)
                            aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtt                                                   >  1:2798375/1‑58 (MQ=255)
                            aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCtt                                                   >  1:265490/1‑58 (MQ=255)
                             gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCg                                                            >  1:235458/1‑48 (MQ=255)
                              ggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCtttt                                                 >  1:774722/1‑58 (MQ=255)
                                aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGAt                                  <  1:3118318/71‑1 (MQ=255)
                                aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGAt                                  <  1:1752660/71‑1 (MQ=255)
                                aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTg                                                >  1:47511/1‑57 (MQ=255)
                                aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGAt                                  <  1:264290/71‑1 (MQ=255)
                                  tAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAgc                                                                  >  1:3176908/1‑36 (MQ=38)
                                  tAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGc                                >  1:3583489/1‑71 (MQ=255)
                                  tAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGc                                >  1:2215089/1‑71 (MQ=255)
                                   aGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAgctgc                                                     <  1:3381983/49‑1 (MQ=255)
                                     cGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGc                                <  1:1702946/68‑1 (MQ=255)
                                     cGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTa                             <  1:2952611/71‑1 (MQ=255)
                                     cGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGATCACTACCCGGATGCCTa                             <  1:2581052/71‑1 (MQ=255)
                                      gTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTAt                            <  1:2832369/71‑1 (MQ=255)
                                        cTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCg                          >  1:2000182/1‑71 (MQ=255)
                                         ttAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAgctgc                                                     >  1:3017219/1‑43 (MQ=255)
                                         ttAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCAc                                           >  1:1838030/1‑53 (MQ=255)
                                         ttAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGa                                   >  1:1575446/1‑61 (MQ=255)
                                           aaGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAg                                                         <  1:1001116/37‑1 (MQ=25)
                                           aaGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGa                                   >  1:761947/1‑59 (MQ=255)
                                           aaGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGc                         <  1:4513309/69‑1 (MQ=255)
                                           aaGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGc                         <  1:3226823/69‑1 (MQ=255)
                                           aaGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGcc                        <  1:3350977/70‑1 (MQ=255)
                                            aGCTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCa                       <  1:1127044/70‑1 (MQ=255)
                                            aGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCg                          >  1:3184563/1‑67 (MQ=255)
                                            aGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCAc                      >  1:4615724/1‑71 (MQ=255)
                                              cTGGCGCGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCg                    <  1:3639719/71‑1 (MQ=255)
                                                 gcgcGAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtc                  <  1:411388/70‑1 (MQ=255)
                                                 gcgcGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcg                 <  1:694616/71‑1 (MQ=255)
                                                  cgcgAGGACCAACGCATTAGTCAGGCGAAGCTGCtt                                                   <  1:3663134/36‑1 (MQ=255)
                                                  cgcgAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcgg                <  1:4038078/71‑1 (MQ=255)
                                                   gcgAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtcggt               >  1:4525507/1‑71 (MQ=255)
                                                   gcgAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGa                                               >  1:3743209/1‑39 (MQ=255)
                                                   gcgAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCg                                     <  1:2762317/49‑1 (MQ=255)
                                                     gAGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCAcc                     <  1:1504644/63‑1 (MQ=255)
                                                     gAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGAt                                  >  1:2851522/1‑50 (MQ=255)
                                                     gAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcggtcg             <  1:1033160/71‑1 (MQ=255)
                                                      aGGACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTAt                            >  1:2522577/1‑55 (MQ=255)
                                                      aGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGCGCACTACCCGGATGCCTATCGCCACCgtcggtcg             >  1:1930372/1‑70 (MQ=255)
                                                      aGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGAt                                  >  1:2712416/1‑49 (MQ=255)
                                                       ggACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCa           <  1:3446939/71‑1 (MQ=255)
                                                        gACCAACGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtcggt               >  1:1967784/1‑66 (MQ=255)
                                                         aCCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGAt                                  <  1:1887364/46‑1 (MQ=255)
                                                           gtaCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATg        <  1:4149013/68‑1 (MQ=255)
                                                           cAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcggtcg             <  1:2606228/65‑1 (MQ=255)
                                                           cAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGc            <  1:4302644/66‑1 (MQ=255)
                                                            aaCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGcc                        <  1:1199960/53‑1 (MQ=255)
                                                             aCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCt                              <  1:81819/46‑1 (MQ=255)
                                                             aCGTATCAGCCAGGCGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg     >  1:2374617/1‑71 (MQ=255)
                                                             aCGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg     >  1:1332678/1‑71 (MQ=255)
                                                               gCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGcc                        <  1:3128400/50‑1 (MQ=255)
                                                                cATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTAt                            <  1:601242/45‑1 (MQ=255)
                                                                 aTTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGt       <  1:104577/65‑1 (MQ=255)
                                                                   |                                                                   
GTCTGTCACATCTTTTCCGCCAGCAGTTAGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGCGAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTT  >  AP009048/71015‑71149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: